Effects of per oral cypermethrin exposure on Bcl-2 expression in granulose cells and antral follicle count of Rattus norvegicus ovaries

Abstract

ABSTRAKObjectives: To determine the effect of oral cypermethrin exposure on the decrease of Bcl-2 expression in granulosa cells and antral follicle count in Rattus norvegicus ovary. Materials and Methods:This was a true experimental study using post-test control group design. This study used 24 Rattus norvegicus which were divided into 4 groups, the control group, treatment group (P1) exposed to cypermethrin 10 mg/kg BW, treatment group (P2) exposed to cypermethrin 15 mg/kg BW and treatment group (P3) exposed to cypermethrin 20 mg/kg BW. The treatment groups were exposed to cypermethrin for 28 days. A surgery was conducted in proestrus phase and ovarian organs were taken. Bcl-2 expression examination was performed by using immunohistochemical method and the antral follicle count was assessed with the Hematoxillin Eosin (HE) Results: This study showed the decrease of Bcl-2 expression in cypermethrin-exposed group compared to control group. There was a decrease in antral follicles count in cypermethrin-exposed group compared to control group. Conclusion: Cypermethrin can decrease Bcl-2 expression and antral follicles count in Rattus norvegicus ovaries.

Cypermethrin induced apoptosis and testicular toxicity by upregulation of p53 in the brain and testis of male rats is alleviated by Sesame oil

“…Several prior reports [29,[50][51][52] presented parallel results from our study, where CYP induced apoptosis and upregulated p53 and Cas3 gene expression besides the down-regulation of Bcl2 expression in neuronal cell and testis tissue. Also, studies of Molavi et al [53] and Indrayanti et al [43], which revealed that CYP induced RNA damage and over-expression of Cas3 and p53 in granulosa and theca cells besides decreased expression of Bcl-2 in the ovaries of CYP exposed group, are in accordance with our results.…”

“…Bcl-2 is one member of the Bcl-2 antiapoptosis protein family. If the anti-apoptotic protein is lower than the pro-apoptotic protein then apoptosis will occur [43].…”

“…Based on literature data and our results there is no doubt that CYP induces testicular toxicity and a neurotoxic impact which is demonstrated by induced apoptosis through up-regulation of p53 and Cas3 besides the down-regulation of Bcl2 expression in the brain and testis tissue. These effects are because of CYP induced oxidative stress which can cause lipid peroxidation [54], cell structure damage and eventually induce DNA damage and apoptosis [43]. These are demonstrated by several studies [23,55].…”