Oocyte cryopreservation/a three year follow up of sixteen births.

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

“…The survival rate achieved in the present study is similar to that previously observed with the 0.3 M sucrose method [7,15,16,[31][32][33][34][35][36][37] and the 0.2 M sucrose method [17,19,21]. In some of the above studies only oocytes with optimal morphology were cryopreserved [37,38] which is not the case in the present study.…”

“…Although parameters such as reduced time between collection and cryopreservation [15] and assisted hatching of subsequent embryos [16] may result in some improvement, the clinical efficiency has remained low (reviewed in [11]). In contrast, modifying the sucrose concentration to 0.2 M during dehydration also increased survival [17][18][19][20] but implantation did not appear to be impaired [19,21] suggesting more potential [11] with this modification, although a reduced implantation rate relative to fresh oocytes has also been reported recently [20]. This again raised the criticism that slow cooling is inappropriate for human oocytes Capsule Equivalent implantation rates for slow cooled mature oocytes dehydrated in 1.5 M propanediol and 0.2 M sucrose at 37°C and fresh oocytes from young women.…”

Oocyte cryopreservation

“…Among the Ͼ150 live births reported in the world literature after oocyte cropreservation, there is a single report of a congenital anomaly, a ventricular septal defect (23). In the same report, no intellectual or developmental deficits were detected in 16 children who were born from cryopreserved oocytes after 3 years of follow-up (23).…”

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

“…To date, more than 1,000 successful live births resulting from cryo-preserved human oocytes have been reported [2][3][4][5][6][7][8][9][10][11][12]. Vitrification seems to be a successful and promising technique for oocyte cryopreservation, resulting in several hundred healthy live births recently [10,11,[13][14][15].…”