Oocyte cryopreservation

Jain, John K.; Paulson, Richard J.

doi:10.1016/j.fertnstert.2006.07.1478

Cited by 161 publications

(127 citation statements)

References 103 publications

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“…The parameters g and j in the model that were determined phenomenologically might contribute to the difference, as was pointed out by Karlsson et al 9,37 For oocyte cryopreservation, the cells are usually frozen at 0.3-2 K/min in the presence of $1.5 M CPAs or cooled ultra-rapidly by plunging directly to liquid nitrogen with $5.5 M CPAs. 60 While the actual cooling rates for ultrarapid cooling to eliminate apparent IIF is still unclear, the cryopreservation protocols used routinely to date seem to agree with the condition predicted by the present model. An additional condition to be considered is the cytotoxicity of CPA, which is more of a concern at higher concentrations.…”

Section: B Comparison With Previous Model With Ideal-solution Assumpsupporting

confidence: 66%

The effect of solution nonideality on modeling transmembrane water transport and diffusion-limited intracellular ice formation during cryopreservation

Zhao¹,

Takamatsu²,

He³

2014

Journal of Applied Physics

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A new model was developed to predict transmembrane water transport and diffusion-limited ice formation in cells during freezing without the ideal-solution assumption that has been used in previous models. The model was applied to predict cell dehydration and intracellular ice formation (IIF) during cryopreservation of mouse oocytes and bovine carotid artery endothelial cells in aqueous sodium chloride (NaCl) solution with glycerol as the cryoprotectant or cryoprotective agent. A comparison of the predictions between the present model and the previously reported models indicated that the ideal-solution assumption results in under-prediction of the amount of intracellular ice at slow cooling rates (<50 K/min). In addition, the lower critical cooling rates for IIF that is lethal to cells predicted by the present model were much lower than those estimated with the ideal-solution assumption. This study represents the first investigation on how accounting for solution nonideality in modeling water transport across the cell membrane could affect the prediction of diffusion-limited ice formation in biological cells during freezing. Future studies are warranted to look at other assumptions alongside nonideality to further develop the model as a useful tool for optimizing the protocol of cell cryopreservation for practical applications. V C 2014 AIP Publishing LLC. [http://dx

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Section: B Comparison With Previous Model With Ideal-solution Assumpsupporting

confidence: 66%

The effect of solution nonideality on modeling transmembrane water transport and diffusion-limited intracellular ice formation during cryopreservation

Zhao¹,

Takamatsu²,

He³

2014

Journal of Applied Physics

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“…However, there are toxic effects of high cryoprotectant concentrations during vitrification; cells must tolerate a short equilibration time and a rapid cooling rate [7]. For this reason, the main effort has been to reduce the volume of vitrification solution that is used [6].…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Ebrahimi

Valojerdi

Eftekhari‐Yazdi

et al. 2010

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. Methods Freshly isolated (control group) and vitrifiedwarmed COCs (cryotop group) were matured in vitro. The expression of pro-and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. Results The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25±3.01% and 51.94±2.7% respectively. The expression rates of proand anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p<0.05). Conclusion Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.

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“…Recently new vitrification techniques have been applied for cryopreservation of porcine oocytes (Jain and Paulson, 2006;Gupta et al, 2007;Huang et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

Varga

Gajdócsi

Makkosné

et al. 2008

Acta Veterinaria Hungarica

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The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 animals representing different colour variations, the preservation of this traditional pig breed is still very important. Vitrification is a potential tool for the preservation of gametes and embryos of these animals. The aim of this study was to investigate the effects of vitrification on the developmental competence of Mangalica (M) and Large White (LW) oocytes following fertilisation. The oocytes were vitrified by the Open Pulled Straw (OPS) method using different concentrations of ethylene glycol and dimethyl sulphoxide as cryoprotectants. After rehydration the oocytes underwent in vitro fertilisation; the resultant zygotes were then cultured in vitro for four days to assess embryonic development. In the first experiment, in vitro maturation of M and LW oocytes was compared. No significant difference was observed in the nuclear maturation rate of LW and M oocytes. In the second experiment, the sensitivity of oocytes to vitrification was examined by evaluating oocyte morphology after thawing. A higher percentage of LW oocytes showed normal morphology compared to M oocytes, indicating that Mangalica oocytes are more sensitive to cryoprotectants than Large White oocytes. After warming and in vitro fertilisation, more than 50% of the oocytes started embryonic development and by the end of the incubation period morula stage embryos had developed in both groups. The results show that the OPS vitrification technique is well suited to preserve Mangalica oocytes and from these oocytes morula embryos can be produced.

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Oocyte cryopreservation

Cited by 161 publications

References 103 publications

The effect of solution nonideality on modeling transmembrane water transport and diffusion-limited intracellular ice formation during cryopreservation

The effect of solution nonideality on modeling transmembrane water transport and diffusion-limited intracellular ice formation during cryopreservation

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

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