Onychomycosis Associated with Exophiala oligosperma in Taiwan

Wen, Yan; Rajendran, Ranjith; Lin, Ying-Fang; Kirschner, Roland; Hu, Sindy

doi:10.1007/s11046-015-9945-7

Cited by 14 publications

(8 citation statements)

References 15 publications

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“…Cylindrocarpons are important pathogens that cause rust rot in ginseng and American ginseng 38 . Most of the studies on Exophiala have reported them as pathogens of animals and humans 39 , 40 . Cladorrhinum is a biological control fungi ( Cladorrhinum foecundissimum ) and can reduce viral diseases caused by Rhizobia and Pythium 31 , and the endophytic bacteria Cladorrhinum foecundissimum can prevent cotton root rot caused by Rhizoctonia solani 41 .…”

Section: Discussionmentioning

confidence: 99%

Auxiliary rapid identification of pathogenic and antagonistic microorganisms associated with Coptis chinensis root rot by high-throughput sequencing

Liao

Huang

et al. 2021

Sci Rep

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Root rot reduces the yield and medical quality of C. chinensis (Cc). Previous studies of Coptis root rot focused on the identification of pathogens and the rhizosphere microbial community composition. The present study aimed to identify potential pathogenic and antagonistic microorganisms associated with root rot based on a high-throughput sequencing technique to prevent this disease. Healthy and diseased Cc in the endosphere and rhizosphere from the same field were collected to investigate the differences in microbiome composition and function. The results showed that the composition and function of microbes were different. The numbers of animal pathogens, soil saprotrophs, plant saprotrophs, and wood saprotrophs in the endosphere of diseased Cc were higher than those in the healthy endosphere and were dominated by Phaeosphaeriaceae, Cladorrhinum, Fusarium, Exophiala, and Melanommataceae. Fusarium, Volutella, Cladorrhinum, Cylindrocarpon, and Exophiala were significantly enriched in the endosphere of the diseased plants. Co-occurrence network analysis showed that Bacillus was negatively correlated with Fusarium, Volutella, and Cylindrocarpon, indicating that Bacillus may be antagonistic microorganisms. To verify the sequencing results, F. solani and F. avenaceum were isolated and verified as pathogens, and 14 Bacillus strains were isolated, which displayed an apparent suppression effect against the two pathogens on PDA medium and detached roots. The strategy of high-throughput sequencing has the potential for the comprehensive identification of pathogenic and antagonistic microorganisms for plant disease. These results provide research ideas and microbial resources for future studies on mitigating or preventing root rot damage to Cc.

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Section: Discussionmentioning

confidence: 99%

Auxiliary rapid identification of pathogenic and antagonistic microorganisms associated with Coptis chinensis root rot by high-throughput sequencing

Liao

Huang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Cylindrocarpon are the important pathogens that cause rust rot in ginseng and American ginseng 38 . Most of the studies on Exophiala are reported as the pathogens of animal and human diseases 39,40 . Cladorrhinum biological control fungi (Cladorrhinum foecundissimumto) can reduce viral diseases caused by Rhizobia and Pythium 31 , and endophytic bacteria Cladorrhinum foecundissimum can prevent cotton root rot caused by Rhizoctonia solani 41 .…”

Section: Discussionmentioning

confidence: 99%

Auxiliary Rapid Identification of Pathogenic and Antagonistic Microorganisms for Coptis Chinensis Root Rot by High-throughput Sequencing

Liao

Huang

et al. 2021

Preprint

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Root rot reduced the yield and medical quality of C. Chinensis. Previous studies of Coptis root rot focused on the identification of pathogens and the rhizosphere microbial community composition. In order to provide more evidence for preventing this disease, the present study was to identify the pathogenic and antagonistic microorganisms based on a high-throughput sequencing technique. The healthy and diseased C. chinensis in the endosphere and rhizosphere from the same field were collected to investigating the differences in microbiome composition and function. The results showed that the composition and function of microbes were different. The animal pathogen, soil saprotroph, plant saprotroph, and wood saprotroph in the endosphere of the diseased C. chinensis were higher than the healthy endosphere, which was dominated by Phaeosphaeriaceae, Cladorrhinum, Fusarium, Exophiala, and Melanommataceae. Fusarium, Volutella, Cladorhinum, Cylindrocarpon, and Exophiala were significantly enriched in the endosphere of diseased plants. Co-occurrence network analysis showed that Bacillus were negatively correlated with Fusarium, Volutella, and Cylindrocarpon, indicating that they may be antagonistic microorganisms. To verify the sequencing results, F. solani and F. avenaceum have been isolated and verified as pathogens, and 14 Bacillus bacteria have been isolated, which displayed an apparent suppression effect against the two pathogens on PDA medium and detached root. The strategy of high-throughput sequencing has the potential for the comprehensive identification of pathogenic and antagonistic microorganisms for plant disease. These results lay the foundation for future studies on mitigating or preventing root rot damage to C. chinensis.

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“…Further analysis is necessary for the identification at the species level, such as analysis of translation elongation factor 1-α, β-tubulin, and actin genes, and analysis of secondary metabolites. However, most studies on Exophiala species have focused on their importance as etiologic agents of diseases in animals and humans (Richards et al, 1978;Zeng and de Hoog, 2008;De Hoog et al, 2011;Najafzadeh et al, 2013;Wen et al, 2015). Because we were not able to identify the species for Exophiala sp.…”

Section: Discussionmentioning

confidence: 99%

The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities

et al. 2020

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Strawberry Fusarium wilt, caused by the virulent fungus Fusarium oxysporum formae speciales fragariae (Fof) is a devastating soil-borne disease that causes severe production losses worldwide, including Japan. Fof is one of the top 10 fungal pathogens that threaten global crop security, and a method to effectively control this pathogen has yet to be found. This study aimed to investigate the effectiveness of dark septate endophytic (DSE) fungi against Fof to develop an efficient, effective, and environmentally friendly approach to improve plant health and fitness. A total of 19 fungal isolates were assessed, out of which three (SK47, SK48, and SK51) were selected based on their effectiveness in disease suppression in controlled growth chamber conditions using a soil system. Isolates SK47, SK48, and SK51 suppressed disease severity by 85.71, 61.90, and 90.48%, respectively. Molecular identification based on highly conserved small subunit (SSU), internal transcribed spacer (ITS), and large subunit (LSU) nrRNA regions identified these isolates as DSE Exophiala sp., Exophiala pisciphila, and Cladophialophora chaetospira, respectively. The sequences were deposited under accession numbers MN811693-MN811695 in the GenBank database. Notably, our results revealed that isolate C. chaetospira SK51 possessed superior growth promotion activities as well as disease suppression by significantly increased plant growth parameters (shoot and root dry mass, chlorophyll content, flower bud initiation, and number of fruit) in comparison to control plants and other two fungal candidates. Root colonization by C. chaetospira SK51 was visualized, and it was confirmed that the symbiosis with strawberry plants occurred successfully. Our results provide new insights in the application of DSE fungus C. chaetospira SK51 as a biocontrol agent on

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Onychomycosis Associated with Exophiala oligosperma in Taiwan

Cited by 14 publications

References 15 publications

Auxiliary rapid identification of pathogenic and antagonistic microorganisms associated with Coptis chinensis root rot by high-throughput sequencing

Auxiliary rapid identification of pathogenic and antagonistic microorganisms associated with Coptis chinensis root rot by high-throughput sequencing

Auxiliary Rapid Identification of Pathogenic and Antagonistic Microorganisms for Coptis Chinensis Root Rot by High-throughput Sequencing

The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities

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