Auxiliary Rapid Identification of Pathogenic and Antagonistic Microorganisms for Coptis Chinensis Root Rot by High-throughput Sequencing

Abstract

Root rot reduced the yield and medical quality of C. Chinensis. Previous studies of Coptis root rot focused on the identification of pathogens and the rhizosphere microbial community composition. In order to provide more evidence for preventing this disease, the present study was to identify the pathogenic and antagonistic microorganisms based on a high-throughput sequencing technique. The healthy and diseased C. chinensis in the endosphere and rhizosphere from the same field were collected to investigating the differences in microbiome composition and function. The results showed that the composition and function of microbes were different. The animal pathogen, soil saprotroph, plant saprotroph, and wood saprotroph in the endosphere of the diseased C. chinensis were higher than the healthy endosphere, which was dominated by Phaeosphaeriaceae, Cladorrhinum, Fusarium, Exophiala, and Melanommataceae. Fusarium, Volutella, Cladorhinum, Cylindrocarpon, and Exophiala were significantly enriched in the endosphere of diseased plants. Co-occurrence network analysis showed that Bacillus were negatively correlated with Fusarium, Volutella, and Cylindrocarpon, indicating that they may be antagonistic microorganisms. To verify the sequencing results, F. solani and F. avenaceum have been isolated and verified as pathogens, and 14 Bacillus bacteria have been isolated, which displayed an apparent suppression effect against the two pathogens on PDA medium and detached root. The strategy of high-throughput sequencing has the potential for the comprehensive identification of pathogenic and antagonistic microorganisms for plant disease. These results lay the foundation for future studies on mitigating or preventing root rot damage to C. chinensis.

Comparative Analysis of Microbial Community Diversity and Dynamics on Diseased Tubers During Potato Storage in Different Regions of Qinghai China

“…The fungal α -diversity in control Group “C” was more abundant than that in treatment Group “A,” but the bacterial diversity was lower. This is opposite to the results of the study on C. chinensis , in which the α diversity of the rhizosphere fungi of the diseased plants was significantly higher than that of the healthy plants, and the α diversity of the bacteria in the endosphere of the diseased plants was significantly higher than that of the healthy plants ( Liao et al, 2020 ). This may be because the method of selection and drying could reduce the fungi brought into the cellar and reproduce in the cellar.…”

“…Further analysis of the functional groups of the fungal community revealed that the plant–pathogens were also abundant in Group “C” of HY, MY and ZL, while the fungal species of Saprotroph were more abundant in HZ, such as soil Saprotroph, dung Saprotroph and plant Saprotroph, etc. This result is consistent with reports of Coptis Chinensis in which the plant pathogens in the rhizosphere of diseased plants were significantly higher than those of healthy plants ( Liao et al, 2020 ). Therefore, these fungi may be the main pathogens of potato and the treatment were significantly reduced the distribution of pathogens.…”