Onychomycoses due to <i>Microascus cirrosus</i> (syn. <i>M. desmosporus</i>)

Vroey, C. De; Lasagni, A.; Tosi, E; Schroeder, Francisca; Song, Micheline

doi:10.1111/j.1439-0507.1992.tb00845.x

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…are commonly reported as etiological agents in onychomycoses, this was the first time the fungus had ever been reported as an infectious agent in Switzerland. On the other hand, Microascus cirrosus had previously been reported as an infectious agent in onychomycoses (14). A unique identification was obtained in 12 cases (Table 3).…”

Section: Resultsmentioning

confidence: 78%

Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism

Verrier

Pronina²,

Peter³

et al. 2012

J Clin Microbiol

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A fast and reliable assay for the identification of dermatophyte fungi and nondermatophyte fungi (NDF) in onychomycosis is essential, since NDF are especially difficult to cure using standard treatment. Diagnosis is usually based on both direct microscopic examination of nail scrapings and macroscopic and microscopic identification of the infectious fungus in culture assays. In the last decade, PCR assays have been developed for the direct detection of fungi in nail samples. In this study, we describe a PCR-terminal restriction fragment length polymorphism (TRFLP) assay to directly and routinely identify the infecting fungi in nails. Fungal DNA was easily extracted using a commercial kit after dissolving nail fragments in an Na 2 S solution. Trichophyton spp., as well as 12 NDF, could be unambiguously identified by the specific restriction fragment size of 5=-end-labeled amplified 28S DNA. This assay enables the distinction of different fungal infectious agents and their identification in mixed infections. Infectious agents could be identified in 74% (162/219) of cases in which the culture results were negative. The PCR-TRFLP assay described here is simple and reliable. Furthermore, it has the possibility to be automated and thus routinely applied to the rapid diagnosis of a large number of clinical specimens in dermatology laboratories.

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Section: Resultsmentioning

confidence: 78%

Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism

Verrier

Pronina²,

Peter³

et al. 2012

J Clin Microbiol

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“…For example, the best-matched species is Microascus cirrosus (Per. Ident = 99.67% by BLAST in NCBI), which causes onychomycosis ( De Vroey et al, 1992 ) and postoperative fungal infection ( Krisher et al, 1995 ; Miossec et al, 2011 ). There are other two putative fungi: Rhizomucor pusillus (with Per.…”

Section: Discussionmentioning

confidence: 99%

RNA-Based Analysis Reveals High Diversity of Plant-Associated Active Fungi in the Atmosphere

et al. 2021

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Fungi are ubiquitous in nature; that is, they are present everywhere on the planet; understanding the active state and functional capacity of airborne microbes associated with health of human, animal, and plant is critical for biosafety management. Here, we firstly and directly proved that there were about 40% active fungi in the air via rRNA amplicon sequencing and imaging flow cytometry simultaneously. Amplicon sequencing analysis showed differences between structures of active and total fungal community; Ascomycota were dominant in the active community, while Basidiomycota have low transcriptional activity across all samples. Notably, plant pathogenic fungi were predominant in the air, and more than 50% were active, including not only several common plant pathogens but also biotrophic fungi (Erysiphe sp. and Microbotryum sp.) and host-specific pathogens, which were generally considered to be inactive after leaving the host. Putative plant pathogens of eight genera were found active across the sampling season, indicating their superior ability to obtain nutrients even in barren nutrient environments. Interestingly, we detected several potentially active unrecorded fungi in China (Diatrype prominens, Septofusidium herbarum, Pseudomicrostroma glucosiphilum, and Uromycladium tepperianum), which suggested that they spread over a long distance by air and may cause diseases under favorable conditions. Our results suggested that maintaining transmission in air is an essential feature of many fungi including plant pathogens regardless of being a biotrophic, hemibiotrophic, or necrotrophic group. Moreover, two potentially active human pathogens and one animal pathogen were captured, which indicated their potential risks. This study provided a new perspective for more comprehensive understanding of airborne fungi, including their multidimensional lifestyle, state, functioning, and potential pathogenic risk. It also laid the foundation for further prediction and management of airborne microbial communities, which would be of interest for public health and agriculture.

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“…M. cirrosus, the teleomorph of Scopulariopsis spp., is a rare opportunistic fungus and commonly found in soil and decaying organic matter. Up to date, there are only six cases of M. cirrosus infections reported, including three cases of systemic infection [ 2 , 3 , 5 ], two case of onychomycosis [ 4 ] and one case of primary cutaneous infection (the present case) (summarized in Table 1 ). Patients with systemic infection all had underlying disease that compromise the immune system.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the rarity of the infection, the appropriate antifungal regimen for M. cirrosus infection has yet to be defined. Early cases of toenail infections showed resistant to systemic treatment with griseofulvin (GSF), ketoconazole (KTZ), and topical application of imidazole (IMZ), miconazole (MCZ) [ 4 ]. However, recent cases with systemic M. cirrosus infection all showed improvement with debridement and antifungal chemotherapy of amphotericin B alone or combined with voriconazole, terbinafine, and caspofungin, as shown in Table 1 [ 2 , 3 , 5 ].…”

Section: Discussionmentioning

confidence: 99%

Primary cutaneous infection due to Microascus cirrosus: a case report

et al. 2018

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BackgroundMicroascus cirrosus, the teleomorph of Scopulariopsis spp., is a saprobic species with a worldwide distribution and rarely causes human infection. In the present paper, we present the first case of primary cutaneous M. cirrosus infection in a Chinese female.Case presentationA 17-year-old female presented with tender ulceration on her left ankle for three months. Histology revealed multiple branching, septate hyphae and moniliform fungal elements in the dermis. Tissue culture grew M. cirrosus, the teleomorph of Scopulariopsis spp., characterized by intercalary and ballooned, chlamydospore-like structures, annellidic and ampulliform conidiogenous cells along with truncated, bullet-shaped, smooth conidia and globose perithecial ascomata with cylindrical necks. Further molecular sequencing confirmed the identification. A diagnosis of primary cutaneous infection due to M. cirrosus was made. Treatment with itraconazole 200 mg per day for 10 weeks achieved significant improvement of the skin lesions.ConclusionsThis case of uncommon mycotic cutaneous infection highlights the importance of mycological examination that help to recognize rare pathogenic fungi.

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Onychomycoses due to Microascus cirrosus (syn. M. desmosporus)

Cited by 7 publications

References 6 publications

Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism

Identification of Infectious Agents in Onychomycoses by PCR-Terminal Restriction Fragment Length Polymorphism

RNA-Based Analysis Reveals High Diversity of Plant-Associated Active Fungi in the Atmosphere

Primary cutaneous infection due to Microascus cirrosus: a case report

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