Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells

Wang, Xin-Ding; Nolan, Kim E.; Irwanto, Rina Ratnasih; Sheahan, Michael B.; Rose, Ray J.

doi:10.1093/aob/mcq269

Cited by 58 publications

(40 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A study by Rose et al [23] on the origin of roots in leaf cultures of Medicago truncatula, showed that vein procambial cells stimulated by auxin gave rise to callus cells that ultimately generated root meristems. Further studies on regeneration in M. truncatula also revealed that somatic embryos in leaf cultures originated from procambial cells or dedifferentiated mesophyll cells that become totipotent [24]. Cell proliferation within the bundle sheath was also observed in leaf explants of H. occidentalis [25], leading to rhizogenesis.…”

Section: Discussionmentioning

confidence: 95%

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Pilarska

Popielarska-Konieczna

Ślesak

et al. 2014

Acta Soc Bot Pol

View full text Add to dashboard Cite

Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.

show abstract

Section: Discussionmentioning

confidence: 95%

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Pilarska

Popielarska-Konieczna

Ślesak

et al. 2014

Acta Soc Bot Pol

View full text Add to dashboard Cite

show abstract

“…4D). Likewise, calli that give rise to somatic embryos have a cellular origin clearly distinct from pericycle or vascular cells in Medicago truncatula (Wang et al, 2011). It was reported that epidermal cell fate in developing leaves of Arabidopsis thaliana (Arabidopsis) can also be overwritten by overexpression of the RWP-RK protein RKD4 in order to initiate embryogenesis (Waki et al, 2011) (Fig.…”

Section: Cellular Origins Of Regenerationmentioning

confidence: 99%

Plant regeneration: cellular origins and molecular mechanisms

et al. 2016

View full text Add to dashboard Cite

Compared with animals, plants generally possess a high degree of developmental plasticity and display various types of tissue or organ regeneration. This regenerative capacity can be enhanced by exogenously supplied plant hormones in vitro, wherein the balance between auxin and cytokinin determines the developmental fate of regenerating organs. Accumulating evidence suggests that some forms of plant regeneration involve reprogramming of differentiated somatic cells, whereas others are induced through the activation of relatively undifferentiated cells in somatic tissues. We summarize the current understanding of how plants control various types of regeneration and discuss how developmental and environmental constraints influence these regulatory mechanisms.

show abstract

“…bras., Brasília, v.46, n.8, p.814-821, ago. 2011 citocininas e auxinas (Wang et al, 2011). Estas últimas mostraram padrões estabelecidos quanto à polaridade de transporte, o que influencia a formação dos novos embriões.…”

Section: Figura 1 Distribuição Temporal De Frequência Da Germinação unclassified

Potencial de inibição da regeneração de raízes e plântulas em sementes germinantes de Eugenia pyriformis

Amador

Barbedo

2011

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi avaliar o potencial de inibição da formação de raízes e plântulas, em sementes germinantes de uvaieira (Eugenia pyriformis), fragmentadas e fissuradas. As sementes foram separadas por tamanho em dois grupos. Cada grupo foi separado em dois subgrupos, um dos quais foi submetido a teste de germinação. As sementes de cada subgrupo foram submetidas a dois tipos de incisão (total ou parcial) e, em seguida, foram avaliadas quanto à produção de raízes e plântulas. Em sementes com incisão parcial, que apresentavam apenas uma plântula desenvolvida, completou-se a incisão até que as metades fossem separadas. A metade com a raiz foi descartada, e sua complementar foi colocada para germinar, para avaliação da produção de raízes e plântulas nessas frações sem raízes. Em todos os experimentos, utilizou-se o delineamento inteiramente casualizado, em arranjo fatorial 2x2 (tamanho das sementes x germinação visível) e 2x4 (tamanho das sementes x tipo de incisão). As sementes fracionadas de uvaieira apresentam potencial para regeneração de raízes e plântulas, e podem produzir mais de uma muda por semente. A germinação inicia processos de inibição da regeneração de novas raízes e plântulas na semente, e a incisão dos cotilédones pode bloquear essa inibição.Termos para indexação: fracionamento, inibidores, produção de mudas, regenerabilidade, uvaieira. Potential for regeneration inhibition of roots and seedlings in germinating Eugenia pyriformis seedsAbstract -The objective of this work was to evaluate the potential for inhibiting the formation of new roots and seedlings from germinating seeds of "uvaieira" (Eugenia pyriformis), fragmented and fissured. Seeds were separated by size into two groups. Each group was separated into two subgroups, one of which was subjected to germination test. Seeds of each subgroup were subjected to two types of incision (total or partial) and, then, evaluated as for the production of roots and seedlings. Seeds with partial incision, which had only one developed plantlet, had the incision completed until the halves were separated. The half fragment containing the root was discarded, and its complementary part was placed to germinate, in order to evaluate the production of roots and seedlings in these fractions without the roots. The design in all the experiments was completely randomized, in a 2x2 factorial arrangement (length x seed visible germination) or 2x4 (seed size x incision type). Fragmented seeds of "uvaieira" shows potential for root and seedling regeneration and can produce more than one seedling per seed. Germination starts inhibition processes of formation of new roots and plantlets, and the incision of cotyledons can block this inhibition.

show abstract

Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells

Cited by 58 publications

References 56 publications

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Plant regeneration: cellular origins and molecular mechanisms

Potencial de inibição da regeneração de raízes e plântulas em sementes germinantes de Eugenia pyriformis

Contact Info

Product

Resources

About