Ontogenetic events in androgenesis of Brazilian barley genotypes

Silva, A. L. S. da; Moraes-Fernandes, M. I. B.; Ferreira, Alfredo Guí

doi:10.1590/s0034-71082000000200016

Cited by 10 publications

(2 citation statements)

References 14 publications

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“…Protocorm-like bodies were first documented by Morel (1960) when the shoot apex of Cymbidium was cultured and later in other orchids (Park et al, 2003;Roy et al, 2007). Protocorm-like bodies are composed of many meristematic centers that are able to differentiate into shoots and roots (da Silva et al, 2000). Protocorm-like bodies, however, are distinguished from somatic embryos by the lack of a single embryonic axis (Norstog, 1979).…”

Section: Discussionmentioning

confidence: 99%

Plant Regeneration through Protocorm-like Bodies Induced from Nodal Explants of Syngonium podophyllum ‘White Butterfly’

Chen¹,

Liu²,

Deng³

et al. 2008

horts

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Syngonium podophyllum ‘White Butterfly’, one of the most popular ornamental foliage plants, is propagated almost exclusively through in vitro shoot culture. Ex vitro rooting, however, has been associated with severe Myrothecium leaf spot (Myrothecium roridum Tode ex Fr.). The objective of this study was to establish a method for regenerating well-rooted plantlets before ex vitro transplanting. Leaf and petiole explants were cultured on a Murashige and Skoog (MS) basal medium supplemented with N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU), N-phenyl-N′-1,2,3-thiadiazol-5-ylurea (TDZ), 6-benzyladenine (BA), or N-isopentenylaminopurine (2iP) with α-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D), respectively. Calli formed from leaf explants cultured on the basal medium supplemented with CPPU or TDZ with 2,4-D or with NAA as well as from petiole explants cultured on the medium supplemented with BA, CPPU, or TDZ with 2,4-D or NAA. The calli, however, failed to differentiate, and shoot organogenesis did not occur. Culture of nodal explants on the MS basal medium supplemented with 9.84 μm 2iP, 8.88 μm BA, 8.07 μm CPPU, or 9.08 μm TDZ with 2.26 μm 2,4-D resulted in the formation of protocorm-like bodies, adventitious shoots, and subsequently well-rooted plantlets. MS basal medium supplemented with 19.68 μm 2iP and 1.07 μm NAA resulted in the highest percentage (92.9%) of nodal explants producing protocorm-like bodies and an average of 16.9 well-rooted plantlets per nodal explant. Adventitious shoots were able to root in the initial induction medium, but better root development occurred after shoots with protocorm-like bodies were transferred onto MS basal medium supplemented with 9.84 μm 2iP and 2.69 μm NAA. Regenerated plantlets were stable and grew vigorously with 100% survival rates after ex vitro transplanting to a container substrate in a shaded greenhouse.

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Section: Discussionmentioning

confidence: 99%

Plant Regeneration through Protocorm-like Bodies Induced from Nodal Explants of Syngonium podophyllum ‘White Butterfly’

Chen¹,

Liu²,

Deng³

et al. 2008

horts

View full text Add to dashboard Cite

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“…Hal ini merupakan tanda dimulainya reprogram-ming dari lintasan gametofitik menjadi sporofitik yang merupakan prinsip dasar androgenesis, sehingga mikrospora tidak akan menjadi sel gamet jantan melainkan akan membentuk sel-sel somatik. Secara bertahap mikrospora embriogenik yang meng-alami pembelahan berulang akan membentuk kalus embriogenik atau embrioid dan selanjutnya tahap diferensiasinya menjadi embrio globuler dan fase-fase selanjutnya berlangsung normal sampai terbentuk planlet (da Silva et al, 2000).…”

Section: Sistem Haploid Dalam Program Pemuliaan Padiunclassified

Kultur Antera untuk Percepatan Perakitan Varietas Padi di Indonesia

Dewi¹,

Purwoko²

2016

J. AgroBiogen

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Rice is a staple food crop in Indonesia, while the need is increasing due to high rice consumption as well as population increase. The problems can be solved through increase of national rice production. Productivity of lowland and upland should be increased intensively and other potential dry area outside Java and Bali Islands should be considered for extending the area of production. Recently, high yielding variety such as semi dwarf variety, hybrid rice, and new plant type of rice were being developed by Indonesian breeders. However, new method is needed to complement conventional breeding method in order to accelerate rice breeding. Anther culture is one of in vitro culture techniques that can be used to accelerate the obtainment of pure lines through doubled-haploids (DHs) regenerated at first generation of culture for less than one year. Thus, application of anther culture in conventional breeding will increase the efficiency of selection process as well as reducing the cost for labour, land and breeder’s time. The obtainment of green plantlets derived from anther culture of indica rice subspecies has been improved by the addition of 1 mM putrescine into induction and regeneration media. Recently, several upland rice lines tolerant to abiotic stresses (i.e. low light intensity and aluminum toxicity) and biotic stresses (i.e. leaf and neck blast), several lowland rice/paddy lines tolerant to biotic stresses (i.e. bacterial leaf blight and blast), and several hybrid parental lines (i.e. male sterile, maintainer and restorer) were obtained in 2-3 years from several rice breeding program involving anther culture. However, potential use anther culture to provide unique genetic material for mapping populations for use in functional genomics and molecular breeding has not been explored. The results indicated that anther culture is a feasible technology that can be used for accelerating rice breeding program in Indonesia.

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Applications of Synthetic Seed Technology for Propagation, Storage, and Conservation of Orchid Germplasms

Gantait

Mitra

2019

Synthetic Seeds

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Ontogenetic events in androgenesis of Brazilian barley genotypes

Cited by 10 publications

References 14 publications

Plant Regeneration through Protocorm-like Bodies Induced from Nodal Explants of Syngonium podophyllum ‘White Butterfly’

Plant Regeneration through Protocorm-like Bodies Induced from Nodal Explants of Syngonium podophyllum ‘White Butterfly’

Kultur Antera untuk Percepatan Perakitan Varietas Padi di Indonesia

Applications of Synthetic Seed Technology for Propagation, Storage, and Conservation of Orchid Germplasms

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