One step purification and characterization of an extracellular α-amylase from marine Vibrio sp.

Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains

“…The stimulatory effect of K + ions (5 and 10 mM) on amylase activity has been documented previously (Kekos and Macris, 1983;Pandey et al, 2000). In relation to Hg 2+ , present data contradicted the results obtained by others (Shih and Labbé, 1995;Aguilar et al, 2000;Cordeiro et al, 2002;Najafi et al, 2005). However, 1 mM Hg 2+ had no inhibitory effect on amylase activity of Bacillus sp.…”

“…In addition, the presence of 5 mM Zn 2+ had a slight stimulatory effect on amylase activity ( (1 mM) showed a slight inhibitory effect on enzyme activity. These results differed from those of previous studies which showed that Ca 2+ Mg 2+ , Mn 2+ and Na + ions at a concentration of 5 mM enhanced amylase activity (Shih and Labbé, 1995;Sarikaya and Gürgün, 2000;Bernhanrdsdotter et al, 2005;Najafi et al, 2005). However, a similar inhibitory effect of heavy metal ions such as Cu 2+ and Zn 2+ on amylase activity of Bacillus sp.…”

Partial Purification of α-amylase Expressed by a Tropical Mutant Strain of Aspergillus niger IFE 08

“…Microbial sources of α-amylases include the fungi (Wu et al, 2004;Rahardjo et al, 2005;Najafi and Kembhavi, 2005). In a recent investigation, it was reported that a strain Aspergillus niger IFE 04 expressed significantly active α-amylase in a defined medium with starch as carbon source and ammonium chloride as nitrogen source of growth .…”

The Effect of Metal Ions and Specific Chemical Reagents on the Activity of Aspergillus flavus var. oryzae and Bacillus subtilis α-Амylases

“…C52, яка втрачала 36 % активності у присутності 5 мМЕГТА [14] та а-амілази Vibrio sp., активність якої знижувалася до 5 % у присутності 15 мМ ЕГТА [19].…”