One-Step Generation of Engineered Drug-Laden Poly(lactic-<i>co</i>-glycolic acid) Micropatterned with Teflon Chips for Potential Application in Tendon Restoration

Shi, Xuetao; Zhao, Yihua; Zhou, Jianhua; Chen, Song; Wu, Hongkai

doi:10.1021/am402388k

Cited by 20 publications

(11 citation statements)

References 55 publications

(85 reference statements)

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“…Tissue engineering provides a potential treatment for a wide range of diseases. The musculoskeletal system, including bone 1 , 2 , tendon/ligament 3 and muscles 4 , 5 , has become the major target for tissue engineering. The efficacy of musculoskeletal tissue engineering relies highly on the scaffolds, which should essentially support cells to form neotissues at the target sites.…”

Section: Introductionmentioning

confidence: 99%

Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration

Liu

Zhou

et al. 2017

Sci Rep

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Electrospun scaffolds with excellent mechanical properties, high specific surface area and a commendable porous network are widely used in tissue engineering. Improving the hydrophilicity and cell adhesion of hydrophobic substrates is the key point to enhance the effectiveness of electrospun scaffolds. In this study, polycaprolactone (PCL) fibrous membranes with appropriate diameter were selected and coated by mussel-inspired poly norepinephrine (pNE). And norepinephrine is a catecholamine functioning as a hormone and neurotransmitter in the human brain. The membrane with smaller diameter fibers, a relative larger specific surface area and the suitable pNE functionalization provided more suitable microenvironment for cell adhesion and proliferation both in vitro and in vivo. The regenerated muscle layer can be integrated well with fibrous membranes and surrounding tissues at the impaired site and thus the mechanical strength reached the value of native tissue. The underlying molecular mechanism is mediated via inhibiting myostatin expression by PI3K/AKT/mTOR hypertrophy pathway. The properly functionalized fibrous membranes hold the potential for repairing muscle injuries. Our current work also provides an insight for rational design and development of better tissue engineering materials for skeletal muscle regeneration.

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Section: Introductionmentioning

confidence: 99%

Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration

Liu

Zhou

et al. 2017

Sci Rep

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“…Cellular alignment can be further quantified by the distribution of nuclear alignment angle, which was defined as the angle between long axis of nuclei and direction of stripes or fibers . Here, alignment angles were calculated based on fluorescence microscope images of cell nuclei so as to compare the guiding effects of stripes and aligned fibers on the orientation of FB and A549 cells when A549/FB cell micropatterns were formed.…”

Section: Resultsmentioning

confidence: 99%

Hierarchical Patterning of Cells with a Microeraser and Electrospun Nanofibers

Jiang

Zhong

et al. 2015

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For tissue engineering applications, it is important to develop fabrication strategies for building models with controlled cell distributions in defined structures. Here, a simple, flexible approach (named the μ-eraser strategy) is developed to construct multicell micropatterns. This approach involves pressing a poly(dimethylsiloxane) stamp to erase cells growing on substrates, and seeding other types of cells. The pressing/seeding process can be conducted in any designed pattern at desired time point. In a proof of concept, multicell micropatterns of human lung adenocarcinoma epithelial A549 cells, murine fibroblast (FB) cells and murine osteoblast (OB) cells are achieved on Petri dishes and electrospun sheets. Besides forming multicell micropatterns, the cell orientation can be regulated by microstripes and alignment of nanofibers. On Petri dishes and random fiber sheets, FB and OB cells align along microstripes, while A549 cells do not. However, when growing on aligned fiber sheets, no matter whether solo-cultured or co-cultured, all cells in micropatterns orient along the fibers. Based on this technique, a platform is built up to investigate rates of cell migration and interinvasion under solo-culture and co-culture systems. It is believed that this μ-eraser strategy has promise for biological, pharmaceutical, and biomedical applications.

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“…Stem cells are mechanosensitive and produce different types of molecules when activated by outer mechanical forces . To differentiate stem cells into different type cells with various functions, it is important to synthesize a scaffold structure that controls the behaviors of cells (including differentiation and migration), with morphologies that use a combination of topological and mechanical cues …”

Section: Multiple Forcesmentioning

confidence: 99%

Mechanophysical Cues in Extracellular Matrix Regulation of Cell Behavior

et al. 2020

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The extracellular matrix (ECM) is a macromolecular network that can provide biochemical and structural support for cell adhesion and formation. It regulates cell behavior by influencing biochemical and physical cues. It is a dynamic structure whose components are modified, degraded, or deposited during connective tissue development, giving tissues strength and structural integrity. The physical properties of the natural ECM environment control the design of naturally or synthetically derived biomaterials to guide cell function in tissue engineering. Tissue engineering is an important field that explores physical cues of the ECM to produce new viable tissue for medical applications, such as in organ transplant and organ recovery. Understanding how the ECM exerts physical effects on cell behavior, when cells are seeded in synthetic ECM scaffolds, is of utmost importance. Herein we review recent findings in this area that report on cell behaviors in a variety of ECMs with different physical properties, i.e., topology, geometry, dimensionality, stiffness, and tension.

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One-Step Generation of Engineered Drug-Laden Poly(lactic-co-glycolic acid) Micropatterned with Teflon Chips for Potential Application in Tendon Restoration

Cited by 20 publications

References 55 publications

Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration

Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration

Hierarchical Patterning of Cells with a Microeraser and Electrospun Nanofibers

Mechanophysical Cues in Extracellular Matrix Regulation of Cell Behavior

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