One-Step Extraction and Hydrolysis of Flavonoid Glycosides in Rape Bee Pollen Based on Soxhlet-Assisted Matrix Solid Phase Dispersion

Tu, Xijuan; Ma, Shuangqin; Gao, Zhaosheng; Wang, Jing; Huang, Shaokang; Chen, Wenbin

doi:10.1002/pca.2699

Cited by 24 publications

(15 citation statements)

References 25 publications

(37 reference statements)

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“…The obtained SALLE extract from the bee pollen showed a high background of matrix interferences. Bee pollen is a complex matrix which contains pigments, nutrients such as proteins, carbohydrates, vitamins, lipids, and various phytochemical compounds [3,39,40]. We observed that the final extract after SALLE (upper ACN phase) was still a light yellow color, though the phase partition had retained a substantial number of matrix compounds in the lower H 2 O phase.…”

Section: Salting-out Assisted Liquid-liquid Extractionmentioning

confidence: 91%

“…As shown in Figure 1b, this HPLC condition was also suitable for the separation of target neonicotinoid pesticides and the inner standard. Since bee pollen is a rich source of phenolic compounds [39,40], there are complicated peaks in the chromatogram of the SALLE extract; as a consequence, peaks of analytes could not be distinguished from the high background of interferences. This means that the SALLE extract required further clean-up to reduce interfering compounds.…”

Section: Salting-out Assisted Liquid-liquid Extractionmentioning

confidence: 99%

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Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

Chen

2020

Molecules

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As the main source of nutrients for the important pollinator honeybee, bee pollen is crucial for the health of the honeybee and the agro-ecosystem. In the present study, a new sample preparation procedure has been developed for the determination of neonicotinoid pesticides in bee pollen. The neonicotinoid pesticides were extracted using miniaturized salting-out assisted liquid-liquid extraction (mini-SALLE), followed by disposable pipette extraction (DPX) for the clean-up of analytes. Effects of DPX parameters on the clean-up performance were systematically investigated, including sorbent types (PSA, C18, and silica gel), mass of sorbent, loading modes, and elution conditions. In addition, the clean-up effect of classical dispersive solid-phase extraction (d-SPE) was compared with that of the DPX method. Results indicated that PSA-based DPX showed excellent clean-up ability for the high performance liquid chromatography (HPLC) analysis of neonicotinoid pesticides in bee pollen. The proposed DPX method was fully validated and demonstrated to provide the advantage of simple and rapid clean-up with low consumption of solvent. This is the first report of DPX method applied in bee pollen matrix, and would be valuable for the development of a fast sample preparation method for this challenging and important matrix.

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Section: Salting-out Assisted Liquid-liquid Extractionmentioning

confidence: 91%

Section: Salting-out Assisted Liquid-liquid Extractionmentioning

confidence: 99%

Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

Chen

2020

Molecules

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“…Each flask was loaded with at least 50 mg of dry plant material with a particle size range of 200–300 µm. Methanol was used as extraction solvent, since it complies with the requirements of the food supplement market and is commonly applied in analytical extraction procedures ensuring a high rutin solubility . Solvent to feed ratios were investigated considering values in the range of 10 to 1000 (w/w), typical for preparative or analytical applications.…”

Section: Methodsmentioning

confidence: 99%

Solvent accessibility limitation by plant matrix compounds in extraction of rutin from Solanum lycopersicum

Lück

Bergs

Jupke

2020

Separation Science Plus

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Extraction procedures for plant‐based secondary metabolites are mainly influenced by choice of solvent, particle size and solvent to feed ratio. For developing an extraction protocol design‐of‐experiments or model‐based approaches have widely been used. Botanical aspects such as the influence of side compounds are less investigated. Either for analytical or preparative purposes, knowledge of the underlying extraction mechanisms is required to improve the process development. In this work, we demonstrate the mechanism of accessibility limitation of rutin by side compounds depending on the solvent to feed ratio. Extraction yields of target and side compounds are analyzed to derive correlations. The role of side compounds as limiting factors for target compound accessibility is demonstrated by performing two extraction steps using three different plant species. The maximum extraction yield of rutin is reached using a solvent to feed ratio of at least 300 for all three investigated plant species, while side compounds in liquid state generally affect the extractability of rutin. Phosphatidylcholines are identified as possible accessibility‐limiting compounds. In conclusion, the yields of target and side compounds strongly depend on the solvent to feed ratio, while side compounds cover the target compound in dry matter, reducing its accessibility.

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“…Recently, we reported the combination of Soxhlet extraction and MSPD to develop a Soxhlet assisted MSPD (SA-MSPD) method [ 60 , 61 ]. In this modification method, sample was blended with silica gel following the original MSPD protocol and loaded into a column of constant pressure funnel.…”

Section: Modification Of Original Mspdmentioning

confidence: 99%

“…Moreover, the introduction of sorbent into the Soxhlet enabled this classical method to be with clean-up ability. More recently, this SA-MSPD method was further combined with acid-hydrolysis for the quantification of flavonoid aglycones in bee pollen [ 61 ]. The acid hydrolysis SA-MSPD procedure accomplished the extraction and hydrolysis of flavonoid glycosides into one step, and provided a more efficient sample preparation method for the quantification of flavonoid aglycones.…”

Section: Modification Of Original Mspdmentioning

confidence: 99%

A Review on the Recent Progress in Matrix Solid Phase Dispersion

Chen

2018

Molecules

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Matrix solid phase dispersion (MSPD) has proven to be an efficient sample preparation method for solid, semi-solid, and viscous samples. Applications of MSPD have covered biological, food, and environmental samples, including both organic and inorganic analytes. This review presents an update on the development of MSPD in the period 2015~June 2018. In the first part of this review, we focus on the latest development in MSPD sorbent, including molecularly imprinted polymers, and carbon-based nanomaterials etc. The second part presents the miniaturization of MSPD, discussing the progress in both micro-MSPD and mini-MSPD. The on-line/in-line techniques for improving the automation and sample throughput are also discussed. The final part summarizes the success in the modification of original MSPD procedures.

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One-Step Extraction and Hydrolysis of Flavonoid Glycosides in Rape Bee Pollen Based on Soxhlet-Assisted Matrix Solid Phase Dispersion

Cited by 24 publications

References 25 publications

Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

Solvent accessibility limitation by plant matrix compounds in extraction of rutin from Solanum lycopersicum

A Review on the Recent Progress in Matrix Solid Phase Dispersion

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