One-step electrospun scaffold of dual-sized gelatin/poly-3-hydroxybutyrate nano/microfibers for skin regeneration in diabetic wound

Sanhueza, Claudia; Hermosilla, Jeyson; Bugallo-Casal, Ana; Silva‐Candal, Andrés da; Taboada, Cristina; Millán, Rosendo; Concheiro, Ángel; Acevedo, Francisca

doi:10.1016/j.msec.2020.111602

Cited by 47 publications

(42 citation statements)

References 44 publications

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“…The rate of tissue regeneration and membrane biodegradation will both rise as the hydrophilicity increases [ 72 ]. Moreover, our findings agrees with the results reported by Sanhueza et al, which indicates that gelatin containing scaffolds has contact angle lower than PHB scaffold [ 90 ].…”

Section: Resultssupporting

confidence: 93%

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

et al. 2022

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The process of secondary intention wound healing includes long repair and healing time. Electrospun nanofibrous scaffolds have shown potential for wound dressing. Biopolymers have gained much attention due to their remarkable characteristics such as biodegradability, biocompatibility, non-immunogenicity and nontoxicity. This study anticipated to develop a new composite metronidazole (MTZ) immobilized nanofibrous scaffold based on poly (3-hydroxy butyrate) (PHB) and Gelatin (Gel) to be utilized as a novel secondary intention wound healing accelerator. Herein, PHB and Gel were mixed together at different weight ratios to prepare polymer solutions with final concentration of (7%), loaded with two different concentrations 5% (Z1) and 10% (Z2) of MTZ. Nanofibrous scaffolds were obtained by manipulating electrospinning technique. The properties of MTZ immobilized PHB/Gel nanofibrous scaffold were evaluated (SEM, FTIR, TGA, water uptake, contact angle, porosity, mechanical properties and antibacterial activity). Additionally, in vitro cytocompatibility of the obtained nanofibrous scaffolds were assessed by using the cell counting kit-8 (CCK-8 assay). Moreover, in vivo wound healing experiments revealed that the prepared nanofibrous scaffold highly augmented the transforming growth factor (TGF-β) signaling pathway, moderately suppressed the pro-inflammatory cytokine (IL-6). These results indicate that MTZ immobilized PHB/Gel nanofibrous scaffold significantly boost accelerating secondary intention wound healing.

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Section: Resultssupporting

confidence: 93%

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

et al. 2022

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“…Thus, various porphyrin complexes of natural and synthetic origin can be used to modify polymer composite systems to achieve special properties and a unique combination of biomedical characteristics. Polymer-based materials are widely developed for creating such composites based on a polymer matrix and a porphyrin complex [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Effect of the Hemin Molecular Complexes on the Structure and Properties of the Composite Electrospun Materials Based on Poly(3-hydroxybutyrate)

et al. 2021

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The creation of innovative fibrous materials based on biodegradable semicrystalline polymers and modifying additives is an urgent scientific problem. In particular, the development of biomedical materials based on molecular complexes and biopolymers with controlled properties is of great interest. The paper suggests an approach to modifying the structure and properties of the composite materials based on poly(3-hydroxybutyrate) (PHB) obtained by the electrospinning method using molecular complexes of hemin. The introduction of 1–5 wt. % of hemin has a significant effect on the supramolecular structure, morphology and properties of PHB-based fibers. Changes in the supramolecular structure intensified with the increasing hemin concentration. On the one hand, a decrease in the fraction of the crystalline phase by 8–10% was observed. At the same time, there is a decrease in the density of the amorphous phase by 15–70%. Moreover, the addition of hemin leads to an improvement in the strength characteristics of the material: the elongation at break increased by 1.5 times, and in the tensile strength, it increased by 3 times. The antimicrobial activity of the hemin-containing composite materials against Escherichia coli and Staphylococcus aureus was confirmed. The obtained materials are proposed to be used in the creation of composite systems for regenerative medicine.

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“…Another interesting scaffold for diabetic wounds was created by Sanhueza et al [ 138 ], who have prepared poly-3-hydroxybutyrate (PHB) and gelatin fibrous constructs. In vitro, the scaffolds exhibited excellent fibroblasts viability and attachment after incubation for 1, 3, and 7 days.…”

Section: Biomaterials Scaffoldsmentioning

confidence: 99%

An Up-to-Date Review of Biomaterials Application in Wound Management

Niculescu

Grumezescu

2022

Polymers

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Whether they are caused by trauma, illness, or surgery, wounds may occur throughout anyone’s life. Some injuries’ complexity and healing difficulty pose important challenges in the medical field, demanding novel approaches in wound management. A highly researched possibility is applying biomaterials in various forms, ranging from thin protective films, foams, and hydrogels to scaffolds and textiles enriched with drugs and nanoparticles. The synergy of biocompatibility and cell proliferative effects of these materials is reflected in a more rapid wound healing rate and improved structural and functional properties of the newly grown tissue. This paper aims to present the biomaterial dressings and scaffolds suitable for wound management application, reviewing the most recent studies in the field.

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One-step electrospun scaffold of dual-sized gelatin/poly-3-hydroxybutyrate nano/microfibers for skin regeneration in diabetic wound

Cited by 47 publications

References 44 publications

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

Effect of the Hemin Molecular Complexes on the Structure and Properties of the Composite Electrospun Materials Based on Poly(3-hydroxybutyrate)

An Up-to-Date Review of Biomaterials Application in Wound Management

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