One hundred and one new simple sequence repeat-based markers for the canine genome

Abstract

One hundred and one new dinucleotide repeat polymorphisms specific for the canine genome have been identified and characterized. Screening of both primary libraries and marker-selected libraries enriched for simple sequence repeats led to the isolation of large numbers of genomic clones that contained (CA)n repeats. Over 200 of these clones were sequenced, and PCR primers that bracket the repeat were developed for those that contained ten or more continuous (CA)n units. This effort led to the production of 101 polymorphic markers, which were assigned to one of four categories depending on their degree of polymorphism. Fifty-four markers were found to be highly or very highly polymorphic as they had four or more alleles when tested on a panel of unrelated dogs. This group of markers will be useful for following inheritance of traits in crosses between dogs.

Genetic differentiation of eastern wolves in Algonquin Park despite bridging gene flow between coyotes and grey wolves

“…All samples were profiled at the mitochondrial DNA (mtDNA) control region with primers described in Wilson et al (2000) and 12 autosomal microsatellite markers (cxx225, cxx200, cxx123, cxx377, cxx250, cxx204, cxx172, cxx109, cxx253, cxx442, cxx410 and cxx147; Ostrander et al, 1993Ostrander et al, , 1995 with conditions described in Wheeldon and White (2009). Gender was determined either by amplification at the Zfx/Zfy intron (Shaw et al, 2003) or with Zfx/Sry primer pairs (Aasen and Medrano, 1990;Fain and LeMay, 1995).…”

Genomic sweep and potential genetic rescue during limiting environmental conditions in an isolated wolf population

“…Specifically, the loci that we used were AHT125, PEZ19 and FH2137 [19], FH2054 and FH2226 [20], AHT121 [21], C05.377 [22] and CXX.20 [23]. Polymerase chain reactions (PCRs) for blood and faecal samples were carried out in 10 ml reactions containing 5 ml of Qiagen Multiplex PCR Master Mix, 1 ml Q-Solution and 0.06 mM of FH2054, 0.08 mM of AHT125, 0.…”

Canine Parentage Testing Based on Microsatellite Polymorphisms.

“…Based on the criteria, an AHT125 marker [1,6], nine CPH markers [3,4] and six CXX markers [2,8,10,12] were selected. In addition, four CXX markers (CXX419, 453, 618 and 629) were tested in this study, although their He and PIC information was not described previously [11].…”