2001
DOI: 10.1292/jvms.63.1209
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Abstract: ABSTRACT. To establish an accurate method for parentage testing in dogs, microsatellite DNA repeat length polymorphisms were examined. We selected twenty microsatellite markers reported previously and examined their application for parentage testing in Beagles and Labrador Retrievers. Heterozygosity (He), Polymorphism Information Content (PIC), the probabilities of Paternity Exclusion (PE) and the combined PE were calculated from allelic frequencies of the markers. All markers amplified by polymerase chain rea… Show more

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Cited by 35 publications
(29 citation statements)
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“…To have an accurate parentage test of dogs, the canine applied genetics committee of the international society for animal genetics has demanded EP values higher than 0.9995 (Halverson and Edwards, 2000). The combined EP values of the parentage testing system based on this study was higher than the recommended value, and similar or greater compared to values from other studies: Poongsan, 0.9925 for 11 loci (Cho and Cho, 2003); German Shepherd, 0.9999 for 19 loci (Zajc and Sampson, 1999) and 0.9946 for 10 loci (Koskinen and Bredbacka, 2000); Beagle, 0.9999 for 20 loci (Ichikawa et al, 2001); Greyhound, 0.9993 for 19 loci (Zajc and Sampson, 1999). As the markers selected in this study were highly polymorphic with high PIC values, sufficient values of EP could be obtained even though the number of investigated markers was smaller than that of other studies.…”
supporting
confidence: 69%
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“…To have an accurate parentage test of dogs, the canine applied genetics committee of the international society for animal genetics has demanded EP values higher than 0.9995 (Halverson and Edwards, 2000). The combined EP values of the parentage testing system based on this study was higher than the recommended value, and similar or greater compared to values from other studies: Poongsan, 0.9925 for 11 loci (Cho and Cho, 2003); German Shepherd, 0.9999 for 19 loci (Zajc and Sampson, 1999) and 0.9946 for 10 loci (Koskinen and Bredbacka, 2000); Beagle, 0.9999 for 20 loci (Ichikawa et al, 2001); Greyhound, 0.9993 for 19 loci (Zajc and Sampson, 1999). As the markers selected in this study were highly polymorphic with high PIC values, sufficient values of EP could be obtained even though the number of investigated markers was smaller than that of other studies.…”
supporting
confidence: 69%
“…Because German Shepherd, Beagle and Greyhound are popular in South Korea and their genetic diversity has been well studied, they were evaluated in this study for the comparison of results with other studies (Zajc et al, 1997;Zajc and Sampson, 1999;Koskinen and Bredbacka, 2000;Ichikawa et al, 2001;Cho, 2005).…”
mentioning
confidence: 99%
“…Another bitch was inseminated with only the cryopreserved semen on Days 6, 7 and 8. Paternity for the delivered pups was examined using microsatellite markers, as described previously [8]. …”
mentioning
confidence: 99%
“…In regard to the polymorphism of Japanese dogs, blood proteins [25] and mitochondrial DNA [19,24,27] have been extensively studied. Recently, several studies utilizing microsatellite markers have been performed [10,14,17,23,26]. In these studies, however, evidence of the Hardy-Weinberg equilibrium (HWE) has not been presented.…”
mentioning
confidence: 99%