On the subzero temperature preservation of fertilized eggs of rainbow trout.

Haga, Yukio

doi:10.2331/suisan.48.1569

Cited by 31 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cryopreservation and vitrification of fish oocytes has been unsuccessful until now due to the difficulties associated with the removal of intercellular water during cooling, toxicity of cryoprotectants, and inhomogeneous freezing and thawing rates (e.g., Haga, 1982;Harvey and Ashwood-Smith, 1982;Stoss and Donaldson, 1983;Rana, 1995;Guan et al, 2008). Therefore, in vitro storage of unfertilized eggs has been the only alternative until now.…”

Section: Discussionmentioning

confidence: 98%

Fish Oocyte Ageing and its Effect on Egg Quality

Samarin

Policar

Lahnsteiner

2015

Reviews in Fisheries Science & Aquaculture

View full text Add to dashboard Cite

In fish, delayed spawning in nature, delayed egg collection in capture and delayed fertilization after egg stripping can lead to oocyte ageing and over-ripening. Oocyte ageing is reported as one of the most important factors affecting the egg quality. During over-ripening, morphological, physiological, biochemical, histological, cellular, and molecular changes occur inside the eggs and ovarian fluid. These alterations can negatively affect the egg fertilization capacity and successive developmental stages. The time period during which eggs remain viable after ovulation or stripping has been reported from a few minutes to a few weeks depending on the fish species and the storage temperature. Although several biomarkers that characterize the over-ripening phenomenon have been defined, the reliability of these parameters in the field of aquaculture is controversial. For future researches, inhibition of oocyte ageing by antioxidants could be a valuable research topic. In the present article, the morphological, physiological, biochemical, histological, cellular, and molecular changes that occur during oocyte over-ripening under in vivo conditions and during in vitro storage are reviewed and possible reasons for the decrease in viability of eggs are presented. Species-specific changes in the egg viability and larval development are also considered.

show abstract

Section: Discussionmentioning

confidence: 98%

Fish Oocyte Ageing and its Effect on Egg Quality

Samarin

Policar

Lahnsteiner

2015

Reviews in Fisheries Science & Aquaculture

View full text Add to dashboard Cite

show abstract

“…). Furthermore, early‐stage embryos are more sensitive to chilling than later‐stage embryos in some fish, including rainbow trout (Haga ), fathead minnow ( Pimephales promelas ), (Cloud et al. ), olive flounder ( Paralichthys olivaceus ) (Kazuyuki et al.…”

Section: Discussionmentioning

confidence: 99%

Optimization Protocol for Storage of Goldfish (Carassius auratus) Embryos in Chilled State

Shaluei

Imanpoor

Shabani

et al. 2013

Reprod Domestic Animals

View full text Add to dashboard Cite

A series of five experiments were conducted to explore suitable conditions for storing of goldfish embryos in a chilled state. The factors studied were embryo stage, storage temperature, physiological saline solutions and goldfish artificial coelomic fluid (GFACF) medium, antibiotics (penicillin and streptomycin), antioxidants (vitamin E, vitamin C), buffer (Hepes, Tris) and BSA (bovine serum albumin). First, goldfish embryos at eight developmental stages were incubated in aerated and dechlorinated tap water at 0 °C for 24 h. Result shows that early developmental stages were most sensitive to chilling. Heartbeat-stage goldfish embryos were chilled at 0, 4 or 8 °C for up to 72 h in water, and chilled storage was possible only for up to 18, 24 and 48 h at 0, 4 and 8 °C, respectively, without a decrease in viability. Chilling of goldfish embryos at 8 °C in GFACF medium and Dettlaff's solution instead of water and other physiological saline solutions prolonged their viability (p < 0.01). Nevertheless, viability of chilled embryos in GFACF medium was slightly, but non-significantly, higher than in Dettlaff's solution. Supplementation of the GFACF medium with antibiotics, Hepes or BSA increased the viability of chilled embryos, but the tested vitamin E analogue Trolox, vitamin C or Tris concentration had no effect on embryo viability. The outcome of this series of experiments shows that heartbeat-stage goldfish embryos could be chilled for 60 h in GFACF supplemented with 25 mm Hepes, 100 U/ml penicillin, 10 μg/l streptomycin and 1 g/l BSA in such a way that embryonic development does not proceed, and viability is not lost.

show abstract

“…in some fishes, including rainbow trout (Haga, 1982), fathead minnow (Cloud, et al 1998), olive flounder (Sasaki, et al 1988) and medaka (Valdez, et al 2005). In contrast to our data Sasaki et al (1988), Liu et al (1993), Gwo et al (1995) and Hagedorn et al (1997) reported that later-stage embryos of red sea-bream, goldfish, red drum respectively are more sensitive to chilling than some earlier stage embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Stage-dependent chilling sensitivity has been reported for many species of fish embryos, including rainbow trout, Oncorhynchus mykiss (Haga, 1982) carp, Cyprinus carpio, (Dinnyes, et al 1998), fathead minnows, Pimephales promelas, (Cloud, et al 1998), goldfish, Carassius auratus, (Liu, et al 1993) and zebrafish, Danio rerio (Zhang and Rawson, 1995). The early developmental stages of the zebrafish were most sensitive to chilling injury (Zhang and Rawson, 1995).…”

Section: Introductionmentioning

confidence: 99%

Preleminary Studies on Cryopreservation of Common Tench, Tinca tinca, Embryos

El-Battawy

Linhart²

2008

Mediterranean Aquaculture Journal

View full text Add to dashboard Cite

Vitrification could provide a promising tool for the cryopreservation of fish embryos. However, in order to achieve cryopreservation using vitrification, chilling sensitivity and cryoprotectants toxicity were determined using tench embryos at four developmental stages (11, 17, 23 and 29-h). Embryos treated with alcalase (2mL 998ml 2mm at 22°C) were exposed to chilling with/without warming. Other embryos were exposed to methanol and glycerol at the concentration of 10% and 20% for periods of 20 min. Thereafter, embryos were incubated at special incubator to determin the hatching rates. Regarding chilling sensitivity, the hatching rates of embryos decreased significantly (P <0.001) after exposure to 0°C at all developmental stage except 29-h stage. The same results were reported after exposure to chilling followed by warming. It was found that the 11-h embryonic stage was most sensitive to chilling preservation. Whereas, the 29-h stage exhibited the least sensitivity, while 17-h and 23-h stages were intermediate in their sensitivity to chilling. The toxicity of methanol increased significantly (P <0.001) with developmental stage for 11-h, 17-h and 23-h stages. The largest change was a reduction in the toxicity of methanol in 29-h embryos. The highest hatching rates of tench embryos were obtained with 29-h embryos using various concentrations of methanol. The survival trends of different stages of tench embryos exposed to glycerol concentrations were approximately similar to those embryos exposed to methanol concentrations except for 11-h embryos which showed no hatching with both glycerol concentrations. Concerning the viability of embryos after vitrification, unfortunately, it could not obtained any viable embryos in any of conditions examined. In conclusion, as it was quite difficult to vitrify the tench embryos during this study using various vitrifying solutions and the method reported by Chen and Tian (2005) and so further studies using other developmental stages, basic, washing and vitrifying solutions are needed to achieve successful cryopreservation.

show abstract

On the subzero temperature preservation of fertilized eggs of rainbow trout.

Cited by 31 publications

References 0 publications

Fish Oocyte Ageing and its Effect on Egg Quality

Fish Oocyte Ageing and its Effect on Egg Quality

Optimization Protocol for Storage of Goldfish (Carassius auratus) Embryos in Chilled State

Preleminary Studies on Cryopreservation of Common Tench, Tinca tinca, Embryos

Contact Info

Product

Resources

About