Abstract:Vitrification could provide a promising tool for the cryopreservation of fish embryos. However, in order to achieve cryopreservation using vitrification, chilling sensitivity and cryoprotectants toxicity were determined using tench embryos at four developmental stages (11, 17, 23 and 29-h). Embryos treated with alcalase (2mL 998ml 2mm at 22°C) were exposed to chilling with/without warming. Other embryos were exposed to methanol and glycerol at the concentration of 10% and 20% for periods of 20 min. Thereafter,… Show more
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