On the role of abscisic acid in seed dormancy of red rice

Gianinetti, Alberto; Vernieri, Paolo

doi:10.1093/jxb/erm198

Cited by 44 publications

(48 citation statements)

References 53 publications

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“…2b). There was no direct correlation of ABA content with the dormancy status of dry or imbibed seeds in red weedy rice (Gianinetti and Vernieri 2007). The qSD12 locus controls seed dormancy through the embryo and/or endosperm tissues (Gu et al 2008).…”

Section: The Weedy Rice Bac Librarymentioning

confidence: 91%

The qSD12 underlying gene promotes abscisic acid accumulation in early developing seeds to induce primary dormancy in rice

Liu

Feng

et al. 2009

Plant Mol Biol

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Seeds acquire primary dormancy during their development and the phytohormone abscisic acid (ABA) is known to play a role in inducing the dormancy. qSD12 is a major seed dormancy quantitative trait locus (QTL) identified from weedy rice. This research was conducted to identify qSD12 candidate genes, isolate the candidates from weedy rice, and determine the relation of the dormancy gene to ABA. A fine mapping experiment, followed by marker-assisted progeny testing for selected recombinants, narrowed down qSD12 to a genomic region of \75 kb, where there are nine predicted genes including a cluster of six transposon/retrotransposon protein genes and three putative (a PIL5, a hypothetic protein, and a bHLH transcription factor) genes based on the annotated Nipponbare genome sequence. The PIL5 and bHLH genes are more likely to be the QTL candidate genes. A bacterial artificial chromosome (BAC) library equivalent to 8-9 times of the haploid genome size was constructed for the weedy rice. One of the two BAC contigs developed from the library covers the PIL5 to bHLH interval. A pair of lines different only in the QTL-containing region of \200 kb was developed as isogenic lines for the qSD12 dormancy and non-dormancy alleles. The dormant line accumulated much higher ABA in 10-day developing seeds than the non-dormant line. In the QTL-containing region there is no predicted gene that has been assigned to ABA biosynthetic or metabolic pathways. Thus, it is concluded that the qSD12 underlying gene promotes ABA accumulation in early developing seeds to induce primary seed dormancy.

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Section: The Weedy Rice Bac Librarymentioning

confidence: 91%

The qSD12 underlying gene promotes abscisic acid accumulation in early developing seeds to induce primary dormancy in rice

Liu

Feng

et al. 2009

Plant Mol Biol

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“…ABA (free form) was determined in crude aqueous extracts of plant material by solid phase radioimmunoassay (RIA) using the monoclonal antibody DBPA-1 [21, 22], which proved to be highly specific for S(+)-ABA [23]. Plant samples were weighed, ground in liquid nitrogen and extracted with 0.5 mL of distilled water overnight at +5°C in the dark.…”

Section: Methodsmentioning

confidence: 99%

Seedling Establishment of Tall Fescue Exposed to Long-Term Starvation Stress

et al. 2016

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In germinating seeds under unfavorable environmental conditions, the mobilization of stores in the cotyledons is delayed, which may result in a different modulation of carbohydrates balance and a decrease in seedling vigor. Tall fescue (Festuca arundinacea Schreb.) caryopses grown at 4°C in the dark for an extended period in complete absence of nutrients, showed an unexpected ability to survive. Seedlings grown at 4°C for 210 days were morphologically identical to seedlings grown at 23°C for 21 days. After 400 days, seedlings grown at 4°C were able to differentiate plastids to chloroplast in just few days once transferred to the light and 23°C. Tall fescue exposed to prolonged period at 4°C showed marked anatomical changes: cell wall thickening, undifferentiated plastids, more root hairs and less xylem lignification. Physiological modifications were also observed, in particular related to sugar content, GA and ABA levels and amylolytic enzymes pattern. The phytohormones profiles exhibited at 4 and 23°C were comparable when normalized to the respective physiological states. Both the onset and the completion of germination were linked to GA and ABA levels, as well as to the ratio between these two hormones. All plants showed a sharp decline in carbohydrate content, with a consequent onset of gradual sugar starvation. This explained the slowed then full arrest in growth under both treatment regimes. The analysis of amylolytic activity showed that Ca2+ played a central role in the stabilization of several isoforms. Overall, convergence of starvation and hormone signals meet in crosstalk to regulate germination, growth and development in tall fescue.

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“…Application of fluridone, an ABA biosynthesis inhibitor, stimulates germination of dormant seeds of N. plumbaginifolia, Arabidopsis, and red rice (Oryza sativa f. spontanea), emphasizing the critical role of ABA in seed dormancy (Grappin et al, 2000;Cadman et al, 2006;Gianinetti and Vernieri, 2007). To evaluate the significance of ABA biosynthesis in SUB1A-mediated seed dormancy, we incubated seeds of LG and the two Ubi:SUB1A transgenics in a series of fluridone solutions ( Figure 3E; see Supplemental Figure 4 online).…”

Section: Sub1a Increases Aba Responsivenessmentioning

confidence: 99%

The Submergence Tolerance Regulator SUB1A Mediates Crosstalk between Submergence and Drought Tolerance in Rice

2010

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Submergence and drought are major constraints to rice (Oryza sativa) production in rain-fed farmlands, both of which can occur sequentially during a single crop cycle. SUB1A, an ERF transcription factor found in limited rice accessions, dampens ethylene production and gibberellic acid responsiveness during submergence, economizing carbohydrate reserves and significantly prolonging endurance. Here, we evaluated the functional role of SUB1A in acclimation to dehydration. Comparative analysis of genotypes with and without SUB1A revealed that SUB1A enhanced recovery from drought at the vegetative stage through reduction of leaf water loss and lipid peroxidation and increased expression of genes associated with acclimation to dehydration. Overexpression of SUB1A augmented ABA responsiveness, thereby activating stressinducible gene expression. Paradoxically, vegetative tissue undergoes dehydration upon desubmergence even though the soil contains sufficient water, indicating that leaf desiccation occurs in the natural progression of a flooding event.Desubmergence caused the upregulation of gene transcripts associated with acclimation to dehydration, with higher induction in SUB1A genotypes. SUB1A also restrained accumulation of reactive oxygen species (ROS) in aerial tissue during drought and desubmergence. Consistently, SUB1A increased the abundance of transcripts encoding ROS scavenging enzymes, resulting in enhanced tolerance to oxidative stress. Therefore, in addition to providing robust submergence tolerance, SUB1A improves survival of rapid dehydration following desubmergence and water deficit during drought.

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On the role of abscisic acid in seed dormancy of red rice

Cited by 44 publications

References 53 publications

The qSD12 underlying gene promotes abscisic acid accumulation in early developing seeds to induce primary dormancy in rice

The qSD12 underlying gene promotes abscisic acid accumulation in early developing seeds to induce primary dormancy in rice

Seedling Establishment of Tall Fescue Exposed to Long-Term Starvation Stress

The Submergence Tolerance Regulator SUB1A Mediates Crosstalk between Submergence and Drought Tolerance in Rice

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