On the nature of visible chromosomal gaps and breaks

Savage, John R. K.

doi:10.1159/000077465

Cited by 43 publications

(24 citation statements)

References 73 publications

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“…They are also wellknown cytogenetic phenomena that have not been sufficiently explained (for review see Savage, 2004). Moreover, there is no scientific field dealing with the third dimension of their formation -a further field for chromosomics.…”

Section: Chromosomics and Chromosomal Breaksmentioning

confidence: 99%

Chromosomics

Claussen¹

2005

Cytogenet Genome Res

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The term “chromosomics” is introduced to draw attention to the three-dimensional morphological changes in chromosomes that are essential elements in gene regulation. Chromosomics deals with the plasticity of chromosomes in relation to the three-dimensional positions of genes, which affect cell function in a developmental and tissue-specific manner during the cell cycle. It also deals with species-specific differences in the architecture of chromosomes, which has been overlooked in the past. Chromosomics includes research into chromatin-modification-mediated changes in the architecture of chromosomes, which may influence the functions and life-spans of cells, tissues, organs and individuals. It also addresses the occurrence and prevalence of chromosomal gaps and breaks.

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Section: Chromosomics and Chromosomal Breaksmentioning

confidence: 99%

Chromosomics

Claussen¹

2005

Cytogenet Genome Res

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“…Irrespective of a long time of research it is not entirely clear how DSBs give rise to CAs [Savage, 1996[Savage, , 1998[Savage, , 2004Natarajan, 2002;Obe et al, 2002;Bryant, 2007].…”

Section: Mechanisms Of Chromosome Aberration Formationmentioning

confidence: 99%

DNA Double Strand Breaks and Chromosomal Aberrations

Obe

Durante

2010

Cytogenet Genome Res

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DNA double strand breaks (DSBs) are ultimate lesions for the formation of chromosomal aberrations (CAs). The formation of CAs is dependent on many factors; some of these are discussed in this review. FISH methodologies have uncovered CA types which cannot be seen with the classical staining methods, and thereby widened our understanding of the origin of CAs. The mobility of DSBs in interphase nuclei is limited. This makes it especially difficult to understand the origin of complex CAs involving many chromosomes. Even using high-resolution mBAND FISH to analyze CAs, the ratio of inter-/intrachromosomal CAs is higher than 1. From this it was postulated that only a subset of DSBs, namely, complex or clustered DSBs give rise mainly to interchromosomal CAs. The finding that endonucleases induce CAs does not fit the idea of complex DSBs being responsible for CA. Probably it is the proximity and not the complexity of DSBs which leads to CA.

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“…It is generally assumed that the secondary constriction is a docking site for nucleolar proteins involved in nucleolus formation and transcription of rDNA and possibly the result of late NOR condensation with respect to the rest of the chromosomes (Jimenez et al 1988;Raska et al 2004). However, terminal and interstitial constrictions are also characteristic of other chromosomal features, e.g., centromeres (Houben and Schubert 2003), fragile sites (Savage 2004), or cold segments (Schweizer 1973;Berg and Greilhuber 1993), which prevents identification of such regions solely based on morphology.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy

et al. 2005

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An atypical peg-like terminal constriction ("peg") on metaphase chromosomes of the plant genus Oziroë could be identified as a nucleolus organizing region (NOR) by detecting 45S rDNA with correlative light microscopy (LM) and scanning electron microscopy (SEM) in situ hybridization (ISH). Using high-resolution 3D analytical SEM, the architecture and DNA distribution of the peg-like NOR were characterized as typical for chromosomes, albeit with significantly smaller chromomeres. ISH procedure was improved for SEM concerning signal localization, labeling efficiency, and structural preservation, allowing 3D SEM analysis of the peg-like NOR structure and rDNA distribution for the first time. It could be shown that implementation of FluoroNanogold markers is an attractive tool that allows efficient immunodection in both LM and SEM. A model is proposed for the peg structure and its mode of condensation.

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On the nature of visible chromosomal gaps and breaks

Cited by 43 publications

References 73 publications

Chromosomics

Chromosomics

DNA Double Strand Breaks and Chromosomal Aberrations

Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy

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