Chromosomics

Claussen, U.

doi:10.1159/000086377

Cited by 37 publications

(34 citation statements)

References 49 publications

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“…Several sperm nuclear alterations have been associated with infertility: sperm DNA damage (Barratt et al, 2010), sperm chromatin immaturity (Oliva, 2006), sperm aneuploidy (Tempest & Griffin, 2004). Role of sperm chromosome topography in male infertility have also been questioned (Finch et al, 2008;Ioannou et al, 2011), and the specific architecture of sperm chromatine seemed to be an integral part of epigenetic mechanism in embryogenesis (Cremer et al, 2004;Claussen, 2005).…”

Section: Introductionmentioning

confidence: 99%

Modification of chromosomal architecture in human spermatozoa with large vacuoles

Perdrix

Travers

Clatot³

et al. 2012

Andrology

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SUMMARYHuman normal spermatozoa present a specific chromatin organization, illustrated particularly by the non-random chromosome positioning. Spermatozoa with large vacuoles, described using motile sperm organelle morphology organization (MSOME), are associated with nuclear alterations, such as abnormal chromatin condensation and aneuploidy. To question a probable association between large nuclear vacuoles and chromatin disorganization, we evaluated chromosomes X, Y and 18 topography in normal spermatozoa (NS) compared with spermatozoa with large vacuoles (SLV). After centrifugation on a gradient density system, 229 NS (spermatozoa presenting a normal nuclear shape and a vacuole area <6.5% of head area) from 10 normal semen samples and 221 SLV (spermatozoa presenting a vacuole area >13% of head area) from 10 semen samples with teratozoospermia were selected using MSOME. A three-colour FISH was carried out using a-satellite centromeric probes for chromosomes X, Y and 18. For each chromosome, longitudinal and spatial positioning of centromeres was analysed. Distribution of each chromosome was non-random in NS and in SLV, whatever the methodology used. Using longitudinal positioning, distribution of chromosome 18 and chromosome Y centromeres did not differ significantly between SLV and NS. On the contrary, chromosome X centromeres were more frequently positioned in the posterior region of sperm nucleus in SLV (p = 0.01). Considering spatial positioning, distributions differed significantly between SN and SLV for chromosome Y (p = 0.02) and chromosome 18 (p < 10 À4) and marginally for chromosome X (p = 0.08). Our study concluded to a modification in chromosomes X, Y and 18 centromere topography between NS and SLV, representing a novel and supplementary evidence to argue chromatin disorganization in SLV.

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Section: Introductionmentioning

confidence: 99%

Modification of chromosomal architecture in human spermatozoa with large vacuoles

Perdrix

Travers

Clatot³

et al. 2012

Andrology

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“…Intranuclear architecture comprises all elements of the cell nucleus, with a particular focus on the topology of chromosomes and their interaction with the nuclear matrix. It has been shown that chromosomes occupy so-called chromosome territories (CT), and topology plays an integral role in maintaining and regulating the function of the genome (Cremer & Cremer 2001, Claussen 2005, Dehghani et al 2005, Foster & Bridger 2005. Chromosome organization in the interphase nucleus is largely regarded to be nonrandom.…”

Section: Introductionmentioning

confidence: 99%

“…During recent years, it has been shown that the topology of the sperm nucleus is highly specific , Zalenskaya & Zalensky 2004: Mudrak et al 2005. This specific architecture has been reported to be an integral part of epigenetic mechanisms to which the sperm contributes in embryogenesis (Cremer et al 2004, Claussen 2005.…”

Section: Introductionmentioning

confidence: 99%

Interindividual differences and alterations in the topology of chromosomes in human sperm nuclei of fertile donors and carriers of reciprocal translocations

2008

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Recently it has been shown that the nucleus of the human spermatozoon appears to possess a specific architecture. The current prevailing view is that spatial organization of the male genome contains information critical for the spermatozoon's function as well as for early embryonic development. The purpose of this study was to determine whether there are alterations in intranuclear localization of centromeres in spermatozoa of chromosomes associated with particular reciprocal chromosome translocations (RCT). We analyzed the longitudinal and spatial localization of centromeres of selected chromosomes in sperm nuclei of four control males with normal karyotypes as well as in six carriers of reciprocal chromosome translocations: t(1;7), t(7;2), t(7;13), t(7;9), t(9;14), and t(4;13). Our study revealed that chromosomes with translocations may have shifted their intranuclear localization and that these translocations may influence the localization of other chromosomes in sperm nuclei. The chromocenter in sperm nuclei of translocation carriers was widened toward the apical side in comparison with chromocenter sites visible in control males. Our study also revealed interindividual differences in the localization of the Y chromosome centromere in the chromocenter area of sperm from fertile individuals.

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“…One of the most intriguing properties of the diploid cell nucleus is its capacity to become transformed into a haploid sperm nucleus (Claussen 2005;Dehghani et al 2005;Foster and Bridger 2005). This transformation is primarily caused by the replacement of histones with protamines, as a result of which sperm chromatin DNA is no longer superhelically twisted.…”

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confidence: 99%

“…Thus, the connection between the conformation of somatic chromatin and spermatozoon-related conformation is manifested in the capacity for bidirectional transformation of both structural types of chromatin. The purpose of such radical conformational change and the resultant sperm chromatin structure are not entirely clear (Cremer et al 2004;Zalenskaya and Zalensky 2004;Ainsworth 2005;Claussen 2005;Kimmins and Sassone-Corsi 2005;Mudrak et al 2005;Shaman and Ward 2006).…”

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confidence: 99%

The use of silver nitrate for the identification of spermatozoon structure in selected mammals

Andraszek

Smalec

2011

Can. J. Anim. Sci.

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Andraszek, K. and Smalec, E. 2011. The use of silver nitrate for the identification of spermatozoon structure in selected mammals. Can. J. Anim. Sci. 91: 239Á246. The spermatozoon is one of the most diversified cell types, and the chromatin of the haploid spermatozoon genome is essentially different from that of the somatic cell as regards its chemical composition, structure and function. Although the structure of spermatozoon chromatin has crucial importance for fertilization and embryo development, standard staining techniques are still predominantly used for identifying semen quality and the assessment of spermatozoa is most often limited to detecting irregularities in their morphological structure. The aim of the present research was to evaluate the usefulness of silver nitrate staining for assessing spermatozoon morphology and identifying spermatozoon structure. Spermatozoa isolated from testes and semen were examined. Silver nitrate staining made it possible to identify many significant details of the morphological structure of the spermatozoon and could be successfully employed in sperm morphology assessments.Key words: Spermatozoa, testicle, spermatogenesis Andraszek, K. et Smalec, E. 2011. Utilisation du nitrate d'argent pour e´tudier la structure des spermatozoı¨des chez certains mammife`res. Can. J. Anim. Sci. 91: 239Á246. Le spermatozoı¨de est l'une des cellules les plus diversifie´es qui soient et la chromatine du spermatozoı¨de haploı¨de diffe`re essentiellement de celle de la cellule somatique pour ce qui est de sa composition chimique, de sa structure et de sa fonction. Bien que la structure de la chromatine dans le spermatozoı¨de joue un roˆle capital dans la fe´condation et le de´veloppement de l'embryon, les techniques de coloration usuelles servent encore principalement a`identifier la qualite´du sperme et l'e´valuation des spermatozoı¨des se limite souvent a`la de´tection des anomalies morphologiques. Les chercheurs voulaient e´tablir l'utilite´de la coloration au nitrate d'argent pour e´tudier la morphologie et pre´ciser la structure des spermatozoı¨des. Ils ont examine´des spermatozoı¨des isole´s des testicules et du sperme. La coloration au nitrate d'argent permet d'identifier de nombreux de´tails morphologiques importants dans la structure des spermatozoı¨des et on pourrait s'en servir pour e´valuer la morphologie du sperme.

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Chromosomics

Cited by 37 publications

References 49 publications

Modification of chromosomal architecture in human spermatozoa with large vacuoles

Modification of chromosomal architecture in human spermatozoa with large vacuoles

Interindividual differences and alterations in the topology of chromosomes in human sperm nuclei of fertile donors and carriers of reciprocal translocations

The use of silver nitrate for the identification of spermatozoon structure in selected mammals

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