On the Inside

Minorsky, Peter V.

doi:10.1104/pp.17.00492

Cited by 2 publications

(18 citation statements)

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“…Furthermore, a triple mutant lacking DPE2, PHS1, and SS4 also revealed one starch granule per chloroplast. Thus, it was concluded that the synthesis of this single starch granule was independent of the pathways absent in the ss4 and dpe2-1/phs1a mutants [ 8 ]. However, the number of starch granules per chloroplast in dpe2-1/phs1a was dependent on the length of the light phase.…”

Section: Introductionmentioning

confidence: 99%

“…However, the number of starch granules per chloroplast in dpe2-1/phs1a was dependent on the length of the light phase. Thus, under short-day conditions, the number of starch granules per chloroplast was mainly one, whereas under long-day conditions, the starch granule number increased to up to four granules per chloroplast [ 8 ]. However, when dpe2-1/phs1a was grown under continuous light, the number of starch granules per chloroplast was 5–7, which is the same as in the wild type [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, in this regard, dpe2-1/phs1a and dpe2-1/phs1a/ss4 were different. Unlike dpe2-1/phs1a , in the triple mutant, the starch granule number remained reduced (zero to four granules per chloroplast) even under continuous light [ 8 ]. Furthermore, starch granule morphology was also affected.…”

Section: Introductionmentioning

confidence: 99%

“…Dpe2-1/phs1a contained larger and more spherical starch granules compared to the wild type and the corresponding single parental mutant lines. Moreover, dpe2-1/phs1a/ss4 revealed large and nearly perfectly spherical starch granules [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…A biochemical explanation for the observed reduction in the number of starch granules per chloroplast in the mutants remains obscure. No referable metabolic alteration was observed [ 7 ], [ 8 ]. However, it was concluded that the regulation of the number of starch granules per chloroplast is associated with the dark phase, particularly with starch degradation [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Reduced starch granule number per chloroplast in the dpe2/phs1 mutant is dependent on initiation of starch degradation

Malinova

Fettke

2017

PLoS ONE

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An Arabidopsis double knock-out mutant lacking cytosolic disproportionating enzyme 2 (DPE2) and the plastidial phosphorylase (PHS1) revealed a dwarf-growth phenotype, reduced starch content, an uneven distribution of starch within the plant rosette, and a reduced number of starch granules per chloroplast under standard growth conditions. In contrast, the wild type contained 5–7 starch granules per chloroplast. Mature and old leaves of the double mutant were essentially starch free and showed plastidial disintegration. Several analyses revealed that the number of starch granules per chloroplast was affected by the dark phase. So far, it was unclear if it was the dark phase per se or starch degradation in the dark that was connected to the observed decrease in the number of starch granules per chloroplast. Therefore, in the background of the double mutant dpe2/phs1, a triple mutant was generated lacking the initial starch degrading enzyme glucan, water dikinase (GWD). The triple mutant showed improved plant growth, a starch-excess phenotype, and a homogeneous starch distribution. Furthermore, the number of starch granules per chloroplast was increased and was similar to wild type. However, starch granule morphology was only slightly affected by the lack of GWD as in the triple mutant and, like in dpe2/phs1, more spherical starch granules were observed. The characterized triple mutant was discussed in the context of the generation of starch granules and the formation of starch granule morphology.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Reduced starch granule number per chloroplast in the dpe2/phs1 mutant is dependent on initiation of starch degradation

Malinova

Fettke

2017

PLoS ONE

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Proteomic analysis of axillary buds of sugarcane at different cutting stages: evidence for alterations in axillary bud gene expression

et al. 2019

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Productivity of sugarcane (Saccharum spp.) crops varies at each cutting stage, reaching critical rates close to the fifth cut (fourth ratoon). Knowledge of proteins involved in the regrowth of sugarcane within the cutting process is important for the development of cultivars with greater longevity. The present study presents new information that the proteome of axillary buds is changed in successive cuts in sugarcane culture. Proteins were identified by UPLC-ESI-Q-TOF (ultra-high-performance liquid chromatography coupled with electrospray ionisation–quadrupole–time-of-flight) mass spectrometry and the Mascot tool. A reduction in the number of proteins was evident in the axillary buds of the fifth cut, as well as a reduction in the number of proteins exclusively detected in the axillary buds with the first cut, an indicator of reduction in the expression of genes that may be essential for the stability of culture development. The reduction in agricultural productivity, sprouting and tillering at advanced stages of the sugarcane crop is accompanied by alterations in axillary-bud gene expression, where <50% of the proteins (47.65%) were detected in both the first (plant cane) and in the fifth (fourth ratoon) cutting stage, whereas >50% (52.35%) were expressed in either the axillary buds of the plant cane or the axillary buds of the fourth ratoon. All MS data are available via jPOST and ProteomeXchange with identifiers JPST000331 and PXD007957, respectively.

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On the Inside

Cited by 2 publications

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Reduced starch granule number per chloroplast in the dpe2/phs1 mutant is dependent on initiation of starch degradation

Reduced starch granule number per chloroplast in the dpe2/phs1 mutant is dependent on initiation of starch degradation

Proteomic analysis of axillary buds of sugarcane at different cutting stages: evidence for alterations in axillary bud gene expression

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