Reduced starch granule number per chloroplast in the dpe2/phs1 mutant is dependent on initiation of starch degradation

Malinova, Irina; Fettke, Joerg

doi:10.1371/journal.pone.0187985

Cited by 21 publications

(22 citation statements)

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“…However, s ex1-8 did not reveal a reduced starch granule number per chloroplast ( Mahlow et al, 2014 ). Similarly, dpe2/phs1/sex1-8 revealed 5-7 starch granules even when plants were grown at 12 h light and 12 h dark conditions ( Malinova and Fettke, 2017 ). Thus, it was concluded that ongoing starch degradation is essential for the observed reduced number of starch granules in dpe2/phs1 ( Malinova and Fettke, 2017 ).…”

Section: Additional Proteins Influencing the Starch Granule Numbermentioning

confidence: 99%

“…In agreement with the observed binding of longer soluble maltodextrins by PTST2, it could be speculated that soluble maltodextrins longer than DP10 are involved in the starch granule initiation, as in dpe2/phs1 no limitation for maltodextrins shorter than DP10 were observed ( Malinova et al, 2014 ). Similarly, when in the background of dpe2/phs1 the starch degradation was blocked by the additional lack of GWD, this glucan balance was again influenced and resulted in an increased number of starch granules with 5-7 granules per chloroplast similar to wild type ( Malinova and Fettke, 2017 ). However, the additional lack of GWD in ss4 has no effect on the starch granule number ( Crumpton-Taylor et al, 2013 ).…”

Section: Metabolic Implications Of Variability In Starch Granule Numbmentioning

confidence: 99%

“…Plants were grown under 12 h ligth/12 h dark unless otherwise specified: transparent bar – continuous light, transparent/grey bar – 14 h light, 10 h dark. 1 Malinova et al, 2014 ; 2 Malinova et al, 2017 ; 3 Roldán et al, 2007 ; 4 D’Hulst and Mérida, 2012 ; 5 Seung et al, 2017 ; 6 Mahlow et al, 2014 ; 7 Malinova and Fettke, 2017 ; 8 Seung et al, 2016 .…”

Section: Metabolic Implications Of Variability In Starch Granule Numbmentioning

confidence: 99%

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Parameters of Starch Granule Genesis in Chloroplasts of Arabidopsis thaliana

et al. 2018

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Starch is the primary storage carbohydrate in most photosynthetic organisms and allows the accumulation of carbon and energy in form of an insoluble and semi-crystalline particle. In the last decades large progress, especially in the model plant Arabidopsis thaliana, was made in understanding the structure and metabolism of starch and its conjunction. The process underlying the initiation of starch granules remains obscure, although this is a fundamental process and seems to be strongly regulated, as in Arabidopsis leaves the starch granule number per chloroplast is fixed with 5-7. Several single, double, and triple mutants were reported in the last years that showed massively alterations in the starch granule number per chloroplast and allowed further insights in this important process. This mini review provides an overview of the current knowledge of processes involved in the initiation and formation of starch granules. We discuss the central role of starch synthase 4 and further proteins for starch genesis and affecting metabolic factors.

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Section: Additional Proteins Influencing the Starch Granule Numbermentioning

confidence: 99%

Section: Metabolic Implications Of Variability In Starch Granule Numbmentioning

confidence: 99%

Section: Metabolic Implications Of Variability In Starch Granule Numbmentioning

confidence: 99%

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Parameters of Starch Granule Genesis in Chloroplasts of Arabidopsis thaliana

et al. 2018

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“…The granules extracted from wild-type Arabidopsis leaves were 338 lenticular in shape with a smooth surface. Several mutations affecting Arabidopsis starch-339 metabolizing enzymes were reported to affect starch granule morphology (Szydlowski et al, 340 2011;Malinova & Fettke, 2017). This is also the case of the ss4 mutants that accumulate 341 large and spherical granules with a smooth surface.…”

Section: Selection Of Pii1 Lines 252mentioning

confidence: 99%

PII1: a protein involved in starch initiation that determines granule number and size in Arabidopsis chloroplast

Vandromme¹,

Spriet²,

Dauvillée³

et al. 2018

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24The initiation of starch granule formation is still poorly understood. However, soluble starch 25 synthase 4 (SS4) appears to be a major component of this process since it is required to 26 synthetize the correct number of starch granules in the chloroplasts of Arabidopsis thaliana 27 plants. A yeast-2-hybrid screen allowed the identification of several putative SS4 interacting 28 partners. We identified the product of At4g32190 locus as a chloroplast-targeted PROTEIN 29 INVOLVED IN STARCH INITIATION (named PII1). Arabidopsis mutants devoid of PII1 30display an alteration of starch initiation process and accumulate, on average, one starch 31 granule per plastid instead of the 5 to 7 granules found in plastids of wild-type plants. These 32 granules are larger than in wild type and they remain flat and lenticular. pii1 mutants display 33 wild-type growth rates and accumulate standard starch amounts. Moreover, starch 34 characteristics, such as amylopectin chain length distribution, remain unchanged. Our results 35 reveal the involvement of PII1 in starch priming process in Arabidopsis leaves through 36 interaction with SS4. 37 38 39 Keywords: starch, starch priming, starch granule size, starch initiation, SS4, PII1, Arabidopsis 40 41 42 43 phenocopy of that of the ss4 mutant, because plant growth and starch granule morphology are 110 unaltered in pii1 mutant compared to wild type. We propose that PII1 is required for starch 111 granule initiation by controlling the catalytic activity of SS4. 112 113 Material and methods 114 115 Plant material and growth conditions 116The ULTImate Y2H TM was carried out by Hybrigenics-services (Paris, France) using 117 SS4 as bait (amino acids 43 to 1040) against a library prepared from one-week-old seedlings. 118Among 125 millions interaction tested, 369, corresponding to 80 different proteins, were 119 positives. Using the ChloroP algorithm prediction (Emanuelsson et al., 1999), we were able to 120 select proteins with predicted chloroplast targeting peptides. We ended-up with six candidates 121 among which PII1 (At4g32190) was selected (Table S1). Hybrigenics-services provides 122interaction results associated to a predicted biological score (PBS). This score indicates the 123 interaction reliability and is divided in 6 different classes (A to F): A: very high confidence in 124 the interaction. B: high confidence in the interaction. C: good confidence of interaction. D: 125 moderate confidence of interaction. E: interaction involving highly connected prey domains. 126This class is subjected to non-specific interactions. F: experimentally proven artifacts. 127 Arabidopsis thaliana lines were obtained from NASC (Nottingham Arabidopsis Stock 128Centre; http://Arabidopsis.info; (Alonso et al., 2003)) or from the collection generated at 129 URGV (INRA of Versailles; (Samson et al., 2002)). Wassilewskija (Ws) and Columbia (Col-130 0) lines were used as wild type references. T-DNA insertion lines used are: pii1-1 131 (SALK_122445); pii1-2 (FLAG_137A02); ss4-1 (GABI_290D11); ss4-2 (FLA...

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“…The granules extracted from WT Arabidopsis leaves were lenticular in shape with a smooth surface. Several mutations affecting Arabidopsis starch‐metabolizing enzymes were reported to affect starch granule morphology (Szydlowski et al ., ; Malinova & Fettke, ). This is also the case of the ss4 mutants that accumulate large and spherical granules with a smooth surface.…”

Section: Resultsmentioning

confidence: 99%

PII1: a protein involved in starch initiation that determines granule number and size in Arabidopsis chloroplast

et al. 2018

View full text Add to dashboard Cite

The initiation of starch granule formation is still poorly understood. However, the soluble starch synthase 4 (SS4) appears to be a major component of this process since it is required to synthesize the correct number of starch granules in the chloroplasts of Arabidopsis thaliana plants. A yeast two-hybrid screen allowed the identification of several putative SS4 interacting partners. We identified the product of At4g32190 locus as a chloroplast-targeted PROTEIN INVOLVED IN STARCH INITIATION (named PII1). Arabidopsis mutants devoid of PII1 display an alteration of the starch initiation process and accumulate, on average, one starch granule per plastid instead of the five to seven granules found in plastids of wild-type plants. These granules are larger than in wild-type, and they remain flat and lenticular. pii1 mutants display wild-type growth rates and accumulate standard starch amounts. Moreover, starch characteristics, such as amylopectin chain length distribution, remain unchanged. Our results reveal the involvement of PII1 in the starch priming process in Arabidopsis leaves through interaction with SS4.

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Reduced starch granule number per chloroplast in the dpe2/phs1 mutant is dependent on initiation of starch degradation

Cited by 21 publications

References 18 publications

Parameters of Starch Granule Genesis in Chloroplasts of Arabidopsis thaliana

Parameters of Starch Granule Genesis in Chloroplasts of Arabidopsis thaliana

PII1: a protein involved in starch initiation that determines granule number and size in Arabidopsis chloroplast

PII1: a protein involved in starch initiation that determines granule number and size in Arabidopsis chloroplast

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