Proteomic analysis of axillary buds of sugarcane at different cutting stages: evidence for alterations in axillary bud gene expression

Maranho, Rone Charles; Benez, Mariana Mancini; Maranho, Gustavo Barizon; Neiverth, Adeline; Santos, Marise Fonseca dos; Carvalho, Ana Lúcia O.; Gonela, Adriana; Mangolin, Claudete Aparecida; Machado, Maria de Fátima Pires da Silva

doi:10.1071/cp19115

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…Quantification by spectral counting is a labelfree quantification method, which is efficient and low cost (Liu et al 2004;Old et al 2005). Proteomic studies have been used to study developmental changes in sugarcane (Maranho et al 2019), and salt stress was shown to induce changes in the proteomic profile of micro-propagated sugarcane shoots (Passamani et al 2017). However, there are no published proteomic studies on sugarcane plants growing in saline soil.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of young sugarcane plants with contrasting salt tolerance

Chiconato

Costa

Balbuena

et al. 2021

Functional Plant Biol.

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Soil salinity affects sugarcane (Saccharum officinale L.) production in arid and semiarid climates, severely reducing productivity. This study aimed to identify differentially regulated proteins in two cultivars that differ markedly in tolerance of saline soil. Plants were grown for 30 days and then subjected to treatments of 0 and 160 mM NaCl for 15 days. The tolerant cultivar showed a 3-fold upregulation of lipid metabolising enzymes, GDSL-motif lipases, which are associated with defence to abiotic stress, and which were not upregulated in the sensitive cultivar. Lipoxygenase was 2-fold upregulated in the tolerant cultivar but not in the sensitive cultivar, as were Type III chlorophyll a/b binding proteins. Other differences were that in the sensitive cultivar, the key enzyme of C 4 photosynthesis, phosphoenolpyruvate carboxylase was downregulated, along with other chloroplast enzymes. Na + concentrations had not reached toxic concentrations in either cultivar by this time of exposure to salt, so these changes would be in response to the osmotic effect of the soil salinity, and likely be in common with plants undergoing drought stress.

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of young sugarcane plants with contrasting salt tolerance

Chiconato

Costa

Balbuena

et al. 2021

Functional Plant Biol.

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Biotechnologies to Improve Sugarcane Productivity in a Climate Change Scenario

Grandis,

Fortirer,

Navarro

et al. 2023

Bioenerg. Res.

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Proteome in regrowth cycles of sugarcane: Absence of proteins to tolerate adverse growth conditions may be related to reduced agricultural productivity

Maranho

Benez

et al. 2023

Acta Sci. Agron.

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One of the main objectives of sugarcane plantations is to increase their longevity without decreasing agricultural productivity. In the present study, we analyzed the proteome of the axillary buds of ‘RB966928’ to investigate possible changes in the number of proteins at different cutting stages. Using tryptic digestion followed by ultra-performance liquid chromatography coupled with high-resolution time-of-flight mass spectrometry, 122 proteins were identified from the proteome of the axillary buds of ‘RB966928’. Of the 122, respectively 97 and 95 proteins were detected at the first and fifth cutting stages, of which 27 and 25 proteins were unique to the respective stage. Proteins that prevent the misfolding of polypeptides generated under stress were exclusively detected at the first cutting stage. Meanwhile, proteins associated with stress responses and disease resistance were exclusively detected at the fifth cutting stage. The present proteomic analysis in the regrowth cycles and axillary bud development of ‘RB966928’ significantly advanced our understanding of the biological processes linked to the reduction of agricultural productivity of sugarcane with the advancement of cutting age. Absence of proteins to tolerate adverse growth conditions at the fifth cutting stage may be related to reduced agricultural productivity, in addition to environmental stress, soil compaction, nutrient availability, cultural practices, and pests or pathogen attacks at different phenological stages of crops.

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Proteomic analysis of axillary buds of sugarcane at different cutting stages: evidence for alterations in axillary bud gene expression

Cited by 3 publications

References 45 publications

Proteomic analysis of young sugarcane plants with contrasting salt tolerance

Proteomic analysis of young sugarcane plants with contrasting salt tolerance

Biotechnologies to Improve Sugarcane Productivity in a Climate Change Scenario

Proteome in regrowth cycles of sugarcane: Absence of proteins to tolerate adverse growth conditions may be related to reduced agricultural productivity

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