On-line submicellar enhanced fluorometric determination of Se(IV) with 2,3-diaminonaphthalene

Speciation of selenium in vitamin tablets using spectrofluorometry following cloud point extraction

“…It was observed that fluorescence intensity increases as the DAN to selenium molar ratio increases from 20 to 50, becomes steady when the ratio is varied from 50 to 500 and decreases slightly afterwards. As reported in literature (Pedro, Andrade, Magni, Tudino, & Bonivardi, 2004) when the ratio of DAN to selenium was increased above 50, blank readings were also increased. Optimum DAN to selenium molar ratio was found to be 50.…”

Biological Availability and Preliminary Selenium Speciation in Selenium-Enriched Mycelium ofLentinula edodes (Berk.)

“…We have chosen the RP HPLC method for quantifying selenium described in our previous paper, the adaptation of the widely used fluorimetric analysis of selenium after derivatization with 2,3-diaminonaphtalene. The big advantage of this method is good selectivity and efficiency as well as low detection limit, comparable with that in AAS method (Hawkes and Kutnik, 1996;Pedro et al, 2004;Turlo et al, 2009). …”

Development of a dual-analyte fluorescent sensor for the determination of bioactive nitrite and selenite in water samples

“…However, despite these assays offer great sensitivity and versatility, they show several drawbacks such as the poor solubility of DAN in water and its toxicity. Moreover, there is a great difficulty in employing the method to detect traces of nitrite and selenite in certain aqueous samples because of high blank values, as well as the fluorescence quenching, and interference by inherent biological substances and colorimetric chemicals, which often complicate the accurate detection of the analytes [32][33][34]. In addition, for the selenium determination, the product formed in aqueous medium must be extracted with organic solvents, such as cyclohexane, in order to improve the sensitivity of the method, since 4,5-benzopiazselenol shows a very low fluorescent quantum yield in water [23,33,35].…”