The present work explores the potential use of the conjugated cationic polyfluorene {[9,9-bis(6'-N,N,N-trimethylammonium)hexyl]fluorene-phenylene} bromide (HTMA-PFP) as a fluorescent membrane marker. To this end, the interaction of the polyelectrolyte with anionic model membranes has been investigated using different biophysical approaches. High affinity interaction was confirmed through alterations in the fluorescence spectrum of HTMA-PFP and by Förster resonance energy transfer (FRET) analysis. Quenching data indicate that once HTMA-PFP interacts with the membrane, it penetrates in the hydrophobic core embedded in the lipid bilayer where it presents high fluorescence quantum yield and photostability. Leakage experiments and dynamic light scattering (DLS) measurements show that the integrity of the lipid vesicles is maintained after polymer incorporation since no vesicle fusion or decomposition into small fragments is detectable. This conclusion is supported by fluorescence microscopy images, which confirm that polyelectrolyte interacts with the vesicle, labeling the lipid membrane without altering its morphology. Further experiments performed as a function of temperature indicate that the polymer is accommodated in the membrane without inducing significant loss of lipid cooperativity and without altering the packing of lipids within the bilayer. Finally, results show that polyelectrolyte fluorescence is sensitive to the large structural changes taking place in the lipid bilayer at the lipid phase transition. All these results confirm the ability of HTMA-PFP to visualize membrane structures and to monitor membrane processes.
The interaction between the conjugated polyelectrolyte poly{[9,9-bis(6'-N,N,N-trimethylammonium)hexyl]fluorene-phenylene} bromide (HTMA-PFP) and human serum albumin (HSA) has been investigated from changes observed in both the spectroscopic properties of HTMA-PFP and the intrinsic fluorescence of HSA. Absorption and fluorescence spectra of HTMA-PFP suggest that HTMA-PFP and HSA form polymer-protein complexes due to electrostatic interactions between the cationic side chains of HTMA-PFP and the negatively charged surface of the protein. Interaction between both macromolecules induces an increase in the fluorescence signal of HTMA-PFP, which suggests that hydrophobic forces also contribute to the polymer-protein complex stabilization. In addition, this interaction causes a decrease in the HSA fluorescence, partially due to static quenching and energy transfer between both macromolecules. Effects of HTMA-PFP on the thermal stability and protein conformation were explored from CD experiments. Results indicate that as polymer is added it binds to HSA and initiates unfolding. This unfolding process induces HTMA-PFP chains to become more extended, disrupting backbone interactions and increasing polymer fluorescence intensity.
The design and development of fluorescent conjugated polyelectrolytes (CPEs) emitting in the red region of the visible spectrum is at present of great interest for bioimaging studies. However, despite the wide variety of CPEs available, stable bright red-emitters remain scarce due to their low solubility and instability in aqueous media, consequently limiting their applications. In this work, we have synthesized and characterized a new red-emitting cationic conjugated polyelectrolyte copoly-{[9,9-bis(6'-N,N,N-trimethylammonium)hexyl]-2,7-(fluorene)-alt-1,4-(naphtho[2,3c]-1,2,5-thiadiazole)} bromide (HTMA-PFNT), based on the incorporation of naphtha[2,3c][1,2,5] thiadiazole on fluorene backbone to increase the bathochromic emission, extending the conjugation length in the polymer backbone. Water stabilization was achieved by binding the polyelectrolyte to two different biological systems which are currently used as nanocarriers: human serum albumin (HSA) and lipid vesicles. Using both systems, stable nanostructures of different composition were obtained and their properties were characterized. The properties of the protein-based nanoparticles are consistent with polyelectrolyte aggregates covered with HSA molecules, while the liposome system is composed of lipid vesicles coated with polyelectrolyte chains partially inserted in the bilayer. Both protein and vesicle structural integrity were not affected after their interaction with HTMA-PFNT, as well as the carrier properties, allowing for the binding and transport of ligands. In addition, the nanoparticles displayed the ability of labeling the cell membrane of living cells. All these results extend the potential applications of these novel multifunctional nanoparticles as therapeutic carriers and bioimaging probes.
This work describes the development of a novel fluorescent biosensor based on the inhibition of alkaline phosphatase (ALP). The biosensor is composed of the enzyme ALP and the conjugated cationic polyfluorene HTMA-PFP. The working principle of the biosensor is based on the fluorescence quenching of this polyelectrolyte by p-nitrophenol (PNP), a product of the hydrolysis reaction of p-nitrophenyl phosphate (PNPP) catalyzed by ALP. Because HTMA-PFP forms unstable aggregates in buffer, with low fluorescence efficiency, previous stabilization of the polyelectrolyte was required before the development of the biosensor. HTMA-PFP was stabilized through its interaction with lipid vesicles to obtain stable blue-emitting nanoparticles (NPs). Fluorescent NPs were characterized, and the ability to be quenched by PNP was evaluated. These nanoparticles were coupled to ALP and entrapped in a sol-gel matrix to produce a biosensor that can serve as a screening platform to identify ALP inhibitors. The components of the biosensor were examined before and after sol-gel entrapment, and the biosensor was optimized to allow the determination of phosphate ion in aqueous medium.
This paper explores the interaction mechanism between the conjugated polyelectrolyte {[9,9-bis(6’-N,N,N-trimethylammonium)hexyl]fluorene-phenylene}bromide (HTMA-PFP) and model lipid membranes. The study was carried out using different biophysical techniques, mainly fluorescence spectroscopy and microscopy. Results show that despite the preferential interaction of HTMA-PFP with anionic lipids, HTMA-PFP shows affinity for zwitterionic lipids; although the interaction mechanism is different as well as HTMA-PFP’s final membrane location. Whilst the polyelectrolyte is embedded within the lipid bilayer in the anionic membrane, it remains close to the surface, forming aggregates that are sensitive to the physical state of the lipid bilayer in the zwitterionic system. The different interaction mechanism is reflected in the polyelectrolyte fluorescence spectrum, since the maximum shifts to longer wavelengths in the zwitterionic system. The intrinsic fluorescence of HTMA-PFP was used to visualize the interaction between polymer and vesicles via fluorescence microscopy, thanks to its high quantum yield and photostability. This technique allows the selectivity of the polyelectrolyte and higher affinity for anionic membranes to be observed. The results confirmed the appropriateness of using HTMA-PFP as a membrane fluorescent marker and suggest that, given its different behaviour towards anionic and zwitterionic membranes, HTMA-PFP could be used for selective recognition and imaging of bacteria over mammalian cells.
In the present work, we have synthesized a novel green-emitter conjugated polyelectrolyte Copoly-{[9,9-bis(6′-N,N,N-trimethylammonium)hexyl]-2,7-(fluorene)-alt-4,7-(2-(phenyl) benzo[d] [1,2,3] triazole)} bromide (HTMA-PFBT) by microwave-assisted Suzuki coupling reaction. Its fluorescent properties have been studied in aqueous media and in presence of model membranes of different composition, in order to explore its ability to be used as a green fluorescent membrane probe. The polyelectrolyte was bound with high affinity to the membrane surface, where it exhibited high fluorescence efficiency and stability. HTMA-PFBT showed lower affinity to zwitterionic membranes as compared to anionic ones, as well as a more external location, near the membrane-aqueous interface. Fluorescence microscopy studies confirmed the interaction of HTMA-PFBT with the model membranes, labelling the lipid bilayer without perturbing its morphology and showing a clear preference towards anionic systems. In addition, the polyelectrolyte was able to label the membrane of bacteria and living mammalian cells, separately. Finally, we explored if the polyelectrolyte can function also as a sensitive probe able of detecting lipid-phase transitions. All these results suggest the potential use of HTMA-PFBT as a green membrane marker for bioimaging and selective recognition of bacteria cell over mammalian ones and as a tool to monitor changes in physical state of lipid membranes.
Solubilisation and stabilization of conjugated polymers, CPs, in aqueous media remains a challenge for many researches trying to extend the biological and environmental applications of this kind of polymers. A number of different alternatives have been considered to address this problem, which are mostly based on the enhancement of the macromolecule polarity, by appending hydrophilic side chains on the polymer backbone. In this work we have investigated a new strategy in which water solubilization is reached by external addition of classical cyclodextrins (α-, β- and γ-CDs) to a solution of non-polar CPs. This strategy allows working with such polymers eliminating the need to synthesize new water-soluble species. The polymer selected for the study was poly-[9,9-bis(6'-bromohexyl-2,7-fluoren-dyil)-co-alt-(benzene-1,4-diy)], PFPBr(2), a polyfluorene previously synthesized in our laboratory. Results show that PFPBr(2) forms fluorescent complexes in aqueous media with β-CD and γ-CD, and much less efficiently with α-CD, probably due to the small size of its cavity. The new PFPBr(2)/CD complexes are stable in time and in a large range of pH, however, at high concentration and temperature, they tend to aggregate and precipitate. In order to increase stabilization and minimize polymer aggregation, complexes were encapsulated inside the pores of silica glasses fabricated using the sol-gel process, obtaining transparent and fluorescent hybrid matrices which were stable in time and temperature. In addition, immobilization of the complexes allows an easy manipulation of the material, thus offering promising applications in the development of biological and chemical sensors.
The nitrate and nitrite content of leaf vegetables (Swiss chard, sea beet, spinach and cabbage), "inflorescence" vegetables (cauliflower) and fruit vegetables (eggplant and vegetable marrow) grown with organic fertilizers have been determined by a modified cadmium–Griess method. Samples were purchased from organic food stores as well as collected directly from an organic farm in Madrid (Spain). Nitrate levels were much higher in the leaf vegetables (especially Swiss chard species; average over the different samples and species of 2778.6 ± 1474.7 mg kg(-1)) than in inflorescence or fruit products (mean values between 50.2 ± 52.6 and 183.9 ± 233.6 mg kg(-1)). Following Swiss chard species, spinach (1349.8 ± 1045.5 mg kg(-1)) showed the highest nitrate content, and nitrite was found above the limit of detection in some samples only (spinach, 4.6 ± 1.0 mg kg(-1); sea beet, 4.2 ± 0.7 mg kg(-1) and Swiss chard, 1.2 ± 0.4 mg kg(-1)). Some vegetables (spinach, cabbage and eggplant) had lower nitrate content in the samples harvested in summer, showing the influence of climatic conditions on the nitrate levels in a plant. The samples taken directly from the organic farm, with the exception of eggplant, had higher or slightly higher average nitrate values than samples purchased in the organic food stores, ranging from 117 to 1077%.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.