On‐line hyphenation of supercritical fluid extraction and two‐dimensional high performance liquid chromatography‐atmospheric pressure chemical ionization tandem mass spectrometer for the analysis of Ganoderma lucidum

Abstract

A novel on-line system combining supercritical fluid extraction (SFE) and two-dimensional high performance liquid chromatography (2D-HPLC) was developed. A trap column and two three-port valves were employed to couple SFE and 2D-HPLC system, which was composed of a CN column and a monolithic silica column, connected by a 10-port dual-position valve. The analytes extracted by supercritical CO2 were completely transferred to the 2D-HPLC system. After separation in two orthogonal modes, the eluents were delivered to APCI-tandem-MS for identification of the samples. In this way, sample preparation, separation, detection, and identification were integrated into an on-line system permitting analysis of the fruiting bodies of Ganoderma lucidum, and at least 73 components in the extract were resolved with calculated peak capacity of up to 1643.

Analysis of triterpenoids in Ganoderma lucidum using liquid chromatography coupled with electrospray ionization mass spectrometry

“…Some methods were established for separation and detection of triterpenoids in G. lucidum [25,26]. In this study, an integrated approach consisting of LC/ESI-MS and tandem mass spectrometry (MS n ) has been used for the identification of triterpenoids in G. lucidum extracts.…”

Application of two-dimensional chromatography in the analysis of Chinese herbal medicines

“…The separation performance can be comprehensively improved in terms of analysis time (130 min) and detected components (a total of 120). Other similar applications based on the coupling of CN and silica monolithic ODS columns also achieved satisfactory results in CHM analysis [68,69]. Moreover, as introduced in the previous section, Guan's team constructed a stop-flow on-line LC-LC system using a vacuum-evaporation loop-type valve interface [60].…”

Monolithic Silica for Fast HPLC: Current Success and Promising Future

“…An example is the application for modern drug discovery which involves efficient parallel synthesis of large numbers of individual compounds, followed by extensive profiling for chemical as well as biological properties. Monolithic silica columns were also used successfully as the second-dimension column in 2D-LC separations [120][121][122][123][124][125][126][127][128][129][130].…”