Application of two-dimensional chromatography in the analysis of Chinese herbal medicines

Use of ion mobility mass spectrometry to enhance cumulative analytical specificity and separation to profile 6‐C/8‐C‐glycosylflavone critical isomer pairs and known–unknowns in medicinal plants

“…and Cao et al . Our observations in the analysis of complex Passiflora plant extracts have shown the utility of the approach can be advantageous in the analysis of medicinal plant species. As a result of IMS acquisition and calibration protocols, in conjunction with processing software, the development of compound specific CCS libraries has increased.…”

“…Cao et al . have explored 2D separation (2D‐GC/2D‐LC), where the major advantages and shortcomings for analysis/quality control of Chinese medicines have been reviewed …”

Introductory Chapter: Mathematical Methods in Liquid Chromatography - The State-of-the-Art Developments and Challenges

“…This is because the peak capacity of the comprehensive system approaches the product of the peak capacities of the first-and seconddimension separations when combining highly orthogonal separation mechanisms [30]. This chromatographic technique (particularly coupled with mass spectrometry detection) has been increasingly applied for the analysis of food samples of both relatively low and highly challenging complexities [31][32][33], complex Chinese herbal medicines (e.g., see Figure 5) [35,36], synthetic polymers and biopolymers [37]. Data processing of comprehensive two-dimensional chromatography is an area of great expansion with reference to data acquisition and handling, peak detection (i.e., two-concept algorithm, inverted watershed algorithm and multiway chemometric methodologies (such as parallel factor analysis model, target finder algorithms and multivariate curve resolution with alternating least squares)), deconvolution of overlapping peaks and data analysis software [38].…”

A high throughput mass spectrometry screening analysis based on two-dimensional carbon microfiber fractionation system

“…In particular, polarity plays a great role in this perspective, making their simultaneous detection and quantification very demanding. Another critical issue that should be addressed is the great difference in the substance content (from mg g -1 to ng g -1 ), that can lead to mask trace compounds or to saturate the limited capacity of the separation columns: most of them remain then unknown or unrevealed [4].…”