On-line high-performance liquid chromatography

Hostettmann, Kurt; Domon, Bruno; Schaufelberger, Daniel; Hostettmann, M.

doi:10.1016/s0021-9673(00)96248-4

Cited by 79 publications

(22 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For the remaining constituents, LC/UV with post-column addition of shift reagents was utilised in order to determine substitution pattern, including the presence of oxygenated groups and the position of the sugar, or other component, linked to the aglycone [11]. Although the derivatisation technique was originally developed for use in conjunction with conventional UV analysis of flavonoids employing methanol as solvent [12], it has also been shown to be appropriate for acidic solvents, such as the formic acid:acetonitrile:methanol mixture utilised in the LC analysis of Maytenus extracts. All phenolic groups, except for those in the peri position to a keto function, are affected by the presence of strong base (sodium hydroxide), while Al 3+ forms a complex with ortho-dihydroxy groups or keto groups having a hydroxyl in the peri position.…”

Section: Resultsmentioning

confidence: 99%

“…Eleven glycosylated flavonoids were determined in M. ilicifolia, the two major components being quercetin-di-rhamno-hexoside (3) and kaempferol-di-rhamno-pentoside (8), neither of which were detected in M. aquifolium. M. ilicifolia also showed the presence of a diglycosylated quercetin derivative (6) and rutin (13), together with four diglycosylated kaempferol derivatives (11,12,16 and 18), two monoglycosylated quercetin derivatives (14 and 15) and quercitrin (19). The presence in M. ilicifolia of rutin, quercitrin and the monoglycosylated flavonoids 14 and 15 has not been previously reported [7].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Identification of flavonols in leaves of Maytenus ilicifolia and M. aquifolium (Celastraceae) by LC/UV/MS analysis

Tiberti

Yariwake

Ndjoko

et al. 2007

Journal of Chromatography B

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of flavonols in leaves of Maytenus ilicifolia and M. aquifolium (Celastraceae) by LC/UV/MS analysis

Tiberti

Yariwake

Ndjoko

et al. 2007

Journal of Chromatography B

Self Cite

View full text Add to dashboard Cite

“…Useful information was also obtained with the post-column addition of UV shift reagents according to a protocol already tested for flavonoids and xanthones. 19,20 The shifted and original UV spectra were overlaid and compared for each peak, and the shifts were interpreted according to data reported in the literature. 21 These analyses provided important complementary information on the structural features and oxygenation pattern for the on-line identification.…”

Section: Lc/uv/ms Analysismentioning

confidence: 99%

On-line LC/UV/MS analysis of flavonols in the three apple varieties most widely cultivated in Brazil

Tiberti¹,

Yariwake²,

Ndjoko³

et al. 2007

J. Braz. Chem. Soc.

Self Cite

View full text Add to dashboard Cite

Este trabalho apresenta a primeira investigação sobre a estrutura química dos flavonóis presentes em cascas das três variedades de maçãs mais cultivadas no Brasil: Gala, Golden e Fuji. As análises foram feitas por CL/UV/EM (cromatografia líquida de alta eficiência com detector UV com arranjo de diodos acoplada à espectrometria de massas), usando adição pós-coluna de reagentes de deslocamento de UV e também espectrometria de massas de múltiplos estágios (EM n ) com ionização "electrospray" no modo negativo. A identificação "on-line" com base nos dados de CL/UV/EM demonstrou a presença de rutina, hiperosídeo, isoquercitrina, quercitrina e outros três derivados de quercetina-3-O-pentosídeo. As análises por CL/UV/EM também demonstraram que as três variedades de maçã têm perfis cromatográficos semelhantes.This work describes the first detailed investigation into the chemical structures of flavonols present in the three apple varieties most commonly cultivated in Brazil: Gala, Golden and Fuji. The analyses were carried out by LC/UV/MS (high-performance liquid chromatography coupled to diode array UV detection and mass spectrometry), using post-column addition of UV shift reagents, as well as multiple stage mass spectrometry (MS n ) with electrospray ionization in the negative ion mode. Rutin, hyperoside, isoquercitrin, quercitrin, quercetin and three other quercetin-3-pentoside derivatives were identified through the characterization of on-line-based LC/UV/MS data. LC/UV/MS analysis also revealed that the three apple cultivars have similar chromatographic profiles. Keywords: apple, flavonols, LC/UV/MS, UV shift reagents IntroductionApple (Malus domestica Borkh., Rosaceae) cultivation stands out as an important and rapidly growing market segment in Brazil. While a few traditional apple varieties have been locally grown, such as Rainha, Soberana and Brazil varieties, other varieties such as Gala, Golden and Fuji have proved superior in terms of growth and productivity, particularly in Brazil's southern and central regions. 1,2 Previous papers have reported on the chemical composition of apple varieties from the USA and from Europe, identifying the main components as esters of caffeic and coumaric acids, procyanidines and flavonols (quercetin glycosylated derivatives). [3][4][5][6][7][8][9][10] In recent years, an increasing number of publications have reported the chemistry of flavonols, especially in view of their biological (antibacterial, antiviral, anti-inflammatory, anti-allergic, anti-trombotic, vasodilatory, anti-mutagenic and neoplastic) properties and their ability to protect against or inhibit the development of cancer when consumed on a regular basis. 11 Notwithstanding the data available from apple samples cultivated in the Northern hemisphere, it must be pointed out that the production of flavonols may vary in response to different environmental conditions. 11 Therefore, the possibility of differences in the chemical composition of flavonols from samples cultivated in the Southern hemisphere cannot be e...

show abstract

“…All three peaks on the mass spectrum showed the same m/z value for the parent ion peak (Figure 2) confirming the same molecular weight of the analyte (indicator dye) when performed in the negative ion mode [M-H + = 433]. [9][10][11][12][13][14][15][16][17][18] A comparison of the HPLC retention times and mass spectral data for the indicator is presented in Table 1.…”

Section: Spectral Analysismentioning

confidence: 77%

Extraction and Evaluation of a New Acid-Base Indicator From Black Gram Husk (Vigna Mungo)

Kumar

Chandraju

Ahmad

et al. 2012

Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-

View full text Add to dashboard Cite

There are several indicators used in different titration procedures for the determination of acids and bases in solutions. Out of these, a widely preferred method is the rapid volumetric analysis because of its simplicity, reliability, and cost-effectiveness. In the present work, the indicator evaluated is extracted from a food waste category, namely black gram (Vigna mungo) husk of India. The new indicator has been characterized as a mixture of three isomeric forms of quercitin-3-O-pentoside, which is a flavonol, using LC/MS/UV data. The indicator color change is pH dependent, pH < 7 (pale pink), pH > 7 (yellow), and pH 7 (colorless), which imparts a sharp color change at the endpoint. The novelty of the method is that the permutations and combinations of different indicators used for the analyses of strong-weak acids/bases can be avoided by adopting this procedure. The efficiency of the indicator is cross-checked by comparing the titration results with those of standard indicators and conductometry. The importance of this approach is that the indicator functions around the neutral pH thus minimizing the possible errors.

show abstract

On-line high-performance liquid chromatography

Cited by 79 publications

References 9 publications

Identification of flavonols in leaves of Maytenus ilicifolia and M. aquifolium (Celastraceae) by LC/UV/MS analysis

Identification of flavonols in leaves of Maytenus ilicifolia and M. aquifolium (Celastraceae) by LC/UV/MS analysis

On-line LC/UV/MS analysis of flavonols in the three apple varieties most widely cultivated in Brazil

Extraction and Evaluation of a New Acid-Base Indicator From Black Gram Husk (Vigna Mungo)

Contact Info

Product

Resources

About