“…Compared to FI, SI is characterized by manifold simplicity, versatility, convenience and speed of calibration, and low consumption of reagents and sample and has been shown to be the most versatile technique, also suitable for automated on-line dilution under nonequilibrium conditions. So far, only a limited number of SI systems for on-line dilution have been reported [12][13][14][15][16][17][18][19][20]. Among them, the single standard calibration and dilution method employing a dilution conduit for creating a concentration gradient of the injected analyte zone seems to be the most attractive and efficient [12].…”