Sampling, Dilution, and Loading Device-coupled HighPerformance Liquid Chromatography Method for Successive On-line Analyses of Major Carbohydrate Products in Immobilized Cellulase Hydrolysate of Paper Cellulose

A new type electrode was formed by modifying a pencil lead with carbon paste and glucose oxidaselinked nano-gold particles. The cyclic voltammetric oxidative potential for glucose measurement with the mediator hydroxymethylferrocene was at 0.33 V. During a 45-day period, the glucose standard addition calibration curve exhibited a wide linear concentration range (0-33.41 mM) accompanied with good linearity (r 2 ³ 0.9985); the sensitivity of the electrode was larger than 5.00 mA mM -1 and the limit of detection (LOD) was below 22.3 mM. This glucose sensor electrode was also usable 228 days later. Mannose, galactose and xylitol produced 5-8% interference and cellobiose, xylose, and arabinose gave 0-1% interference. The glucose produced from the waste Cinnamomum caphora tree branch fiber hydrolysis was successively analyzed by the electrode for 4 days. The analysis accuracy of the electrode method by the spike experiment was up to 98.6% and the precision of measurement for this method was below 5.3%. The glucose content in the immobilized enzyme hydrolysate of the waste tree branches by the electrode method was comparable to that of the HPLC method.

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Automated zone‐sampling dilution by coupling sequential injection analysis to high‐throughput HPLC for the direct determination of gemfibrozil

Tzanavaras

Themelis

Rigas³

2009

J of Separation Science

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A novel automated analytical scheme for the rapid determination of gemfibrozil in drug dissolution samples is reported. The procedure is based on direct coupling of a low pressure continuous flow technique such as sequential injection analysis (SI) to HPLC. SI performs automated dilution of the samples based on zone sampling and fills on-line the loop of the high pressure injection valve. Rapid separation of the analyte from the samples' matrix can be achieved in less than 1.0 min, by using a short RP monolithic column (25x4.6 mm id) at a flow rate of 2 mL/min. The SI and HPLC parts of the setup operate independently: during HPLC separation the next sample is treated by SI. This way a maximum throughput of 60 samples per hour is achieved allowing the complete analysis of a batch of six dissolution samples within 12-18 min based on the replicates. The proposed method was validated in terms of linearity, LOD and LOQ, precision, selectivity and accuracy. Its applicability was tested during quality control of four validation batches of a gemfibrozil-containing formulation. The results were in good agreement with the HPLC method proposed by the US Pharmacopeia.

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Sampling, Dilution, and Loading Device-coupled HighPerformance Liquid Chromatography Method for Successive On-line Analyses of Major Carbohydrate Products in Immobilized Cellulase Hydrolysate of Paper Cellulose

Cited by 12 publications

References 16 publications

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Biosensor with Nano‐gold Particle Modified Pencil Lead Carbon Electrode for Long‐term Glucose Monitoring of Waste Tree Branch Hydrolysis

Automated zone‐sampling dilution by coupling sequential injection analysis to high‐throughput HPLC for the direct determination of gemfibrozil

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