Olfactory cell-based biosensor: A first step towards a neurochip of bioelectronic nose

Liu, Qingjun; Hua, Cunqing; Xu, Ying; Li, Yan; Rong, Li; Wang, Ping

doi:10.1016/j.bios.2006.01.016

Cited by 138 publications

(95 citation statements)

References 20 publications

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“…LAPS test setup is similar to the system we previously reported (Xu et al, 2005;Liu et al, 2006;Zhang et al, 2008). Fig.1 shows the schematic diagram of the LAPS test setup.…”

Section: Test Setup and Measurementmentioning

confidence: 99%

“…LAPS has many advantages such as convenience, simplicity, low cost, and addressable measurement. There are several reports on the research of using LAPS as a cell-semiconductor hybrid system to monitor the extracellular potentials of cells cultured on the surface of LAPS chip (Ismail et al, 2003;Stein et al, 2004;Xu et al, 2005;Liu et al, 2006;Zhang et al, 2008). Fig.1 shows the basic principle of LAPS detecting extracellular potential of OSNs cultured on it.…”

Section: Introductionmentioning

confidence: 99%

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Response enhancement of olfactory sensory neurons-based biosensors for odorant detection

Chen

Qing

et al. 2009

J. Zhejiang Univ. Sci. B

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This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed on the basis of the light addressable potentiometric sensor (LAPS), in which rat OSNs were cultured on the surface of LAPS chip and served as sensing elements. LY294002, the specific inhibitor of phosphatidylinositol 3-kinase (PI3K), was used to enhance the responses of OSNs to odorants. The responses of OSNs to odorants with and without the treatment of LY294002 were recorded by LAPS. The results show that the enhancive effect of LY294002 was recorded efficiently by LAPS and the responses of this OSNs-LAPS hybrid biosensor were enhanced by LY294002 by about 1.5-fold. We conclude that this method can enhance the responses of OSNs-LAPS hybrid biosensors, which may provide a novel strategy for the bioelectrical signal monitor of OSNs in biosensors. It is also suggested that this strategy may be applicable to other kinds of OSNs-based biosensors for cellular activity detection, such as microelectrode array (MEA) and field effect transistor (FET).

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“…LAPS test setup is similar to the system we previously reported (Xu et al, 2005;Liu et al, 2006;Zhang et al, 2008). Fig.1 shows the schematic diagram of the LAPS test setup.…”

Section: Test Setup and Measurementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Response enhancement of olfactory sensory neurons-based biosensors for odorant detection

Chen

Qing

et al. 2009

J. Zhejiang Univ. Sci. B

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“…LAPSs are detectors that utilize the photoconductivity of semiconductors to measure voltages on surfaces. (54) When an appropriate amount of light irradiates LAPSs, electron-hole pairs emerge owing to the intrinsic absorption of the semiconductor. When the semiconductor surface in contact with an insulation layer is in a state of depletion, under the action of the strong electric field in the depletion region, the photoinduced electrons and the electron holes, which had entered the depletion region through diffusion, are separated and then gather on both sides of the depletion region.…”

Section: Biomimetic Olfactory Sensors Based On Light Addressable Potementioning

confidence: 99%

Recent Advances in Olfactory Receptor (OR) Biosensors and Cell Signaling Cascade Amplification Systems

2018

Sensors and Materials

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There are a variety of methods to detect odorant molecules, including gas chromatography (GC) and its related coupled techniques, electronic noses, and olfactory receptor (OR)-based biosensors. Nevertheless, studies in which cell and cell signaling amplifier systems were directly utilizied to detect odors are very few. In this review, we aim to provide some references and suggestions for researchers in related fields by summarizing OR sensors and olfactory cell signaling cascade amplifier systems. In this paper, we summarize the detection methods for odorants, introduce the research and progress on OR sensors, and present a proposal of utilizing cell or tissue signaling cascade amplifier systems to amplify electrochemical signals and the use of the G protein signaling cascade amplifier system to prepare electrochemical biosensors. In recent years, the detection technology of electrochemical sensors has matured. Meanwhile, olfactory sensation within organisms basically transfer nerve signals or metabolic endocrine signals in the form of electrochemical signals. Therefore, ORs combined with cell signaling cascade amplifier systems, as well as electrochemical sensors of signal cascade amplifier systems, have incredible prospects for development and application. The proposal of OR electrochemical biosensors and their cell signaling cascade amplifier systems, provides a new idea and a quantitative method for the detection and assessment of odorants and their physiologic processes in the nervous system.

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“…The multivariate data can be processed by chemometric software to visualise clusters that describe the differences between samples, or can be presented to a pattern recognition engine (typically a neural network) to classify the samples of coffee automatically, based on previously seen exemplar patterns. (Liu et al, 2006)). (b) When OR proteins are extracted they can be immobilised on to transducers such as quartz crystal microbalances that produce a change in frequency when ORs are exposed to a pulse of an odour (used by (Sankaran, Panigrahi and Mallik, 2011)).…”

Section: Figurementioning

confidence: 99%

Towards bionic noses

Persaud

2017

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Olfactory cell-based biosensor: A first step towards a neurochip of bioelectronic nose

Cited by 138 publications

References 20 publications

Response enhancement of olfactory sensory neurons-based biosensors for odorant detection

Response enhancement of olfactory sensory neurons-based biosensors for odorant detection

Recent Advances in Olfactory Receptor (OR) Biosensors and Cell Signaling Cascade Amplification Systems

Towards bionic noses

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