Response enhancement of olfactory sensory neurons-based biosensors for odorant detection

Abstract

This paper presents a novel strategy for the response enhancement of olfactory sensory neurons (OSNs)-based biosensors by monitoring the enhancive responses of OSNs to odorants. An OSNs-based biosensor was developed on the basis of the light addressable potentiometric sensor (LAPS), in which rat OSNs were cultured on the surface of LAPS chip and served as sensing elements. LY294002, the specific inhibitor of phosphatidylinositol 3-kinase (PI3K), was used to enhance the responses of OSNs to odorants. The responses of OSNs to odorants with and without the treatment of LY294002 were recorded by LAPS. The results show that the enhancive effect of LY294002 was recorded efficiently by LAPS and the responses of this OSNs-LAPS hybrid biosensor were enhanced by LY294002 by about 1.5-fold. We conclude that this method can enhance the responses of OSNs-LAPS hybrid biosensors, which may provide a novel strategy for the bioelectrical signal monitor of OSNs in biosensors. It is also suggested that this strategy may be applicable to other kinds of OSNs-based biosensors for cellular activity detection, such as microelectrode array (MEA) and field effect transistor (FET).

PI-3 kinase pathway can mediate the effect of TGF-β1 in inducing the expression of SHARP-2 in LLC-PK1 cells

“…to investigate the possibilities. Our data showed that one of the PI-3 kinase inhibitors, LY294002 (Wu et al, 2009), blocked the effect of TGF-β1 in LLC-PK1 cells. It is unlikely that this was a systemic inhibitory effect of LY294002, or a non-specific effect, because it took effect even at 10 µmol/L, a very low concentration that generally has no systemic effect on mRNA expression.…”

Biosensors for Odor Detection: A Review

“…They also include some synthetic substances similar to biological substances, such as aptamers, peptides, and molecularly imprinted polymers. The biological materials used in odor biosensors are mainly extracted from the creatures' olfactory systems, for example, olfactory epithelium [32], olfactory sensory neuron (OSN) [33], olfactory receptor (OR) protein [34][35][36], and odorant binding protein (OBP) [37,38]. These odor biosensors have higher sensitivity and selectivity towards their ligands than conventional odor sensors, and also they are not sensitive to temperature and humidity changes.…”

“…Biosensors 2023, 13, x FOR PEER REVIEW 2 of 23 creatures' olfactory systems, for example, olfactory epithelium [32], olfactory sensory neuron (OSN) [33], olfactory receptor (OR) protein [34][35][36], and odorant binding protein (OBP) [37,38]. These odor biosensors have higher sensitivity and selectivity towards their ligands than conventional odor sensors, and also they are not sensitive to temperature and humidity changes.…”

A biologically inspired model for pattern recognition

“…They are useful in understanding the signal processing mechanism of olfactory systems. Therefore many mathematical models of olfactory systems have been applied to pattern recognition, often with remarkable results, which help to understand the olfactory information processing (Liljenström and Wu, 1995;Kozma et al, 2003;Gonzalez et al, 2007;Li G. et al, 2007;Li X. et al, 2006;Ma and Krings, 2009;Wu et al, 2009).…”