Ointment-Based Combination of Dittrichia viscosa L. and Marrubium vulgare L. Accelerate Burn Wound Healing

Abstract

Burns constitute a major challenge in medical science, and plants can be part of the solution. Dittrichia viscosa L. (Asteraceae) and Marrubium vulgare L. (Lamiaceae) are widely used in Moroccan traditional medicine to treat several diseases and possess high potency to cure wounds. This study aimed to investigate in vivo the analgesic, anti-inflammatory, and burn-healing effects of both plants and their mixture. The hydro-ethanolic extract of both plants was analyzed using high-performance liquid chromatography with diode-array detection (HPLC-DAD). Burns were conducted on dorsal part of rats, and the wound healing process was evaluated during 21 days. Gallic acid, caffeic acid, ferulic acid, and quercetin were identified in M. vulgare extract. The analysis recorded the presence of caffeic acid, rosmarinic acid, rutin, and quercetin in D. viscosa. The group treated with the mixture showed the lowest abdominal contractions (30.4 ± 7.52) with the highest percentage of inhibition (69.12 ± 7.04%). The inhibition of paw inflammation for M. vulgare was 47.65%, followed by D. viscosa (33.86%) and the mixture (30.41%). The mixture showed the highest wound contraction at day 7 (33.16 ± 14.33%) and day 14 (87.54 ± 3.98%). D. viscosa showed the highest wound contraction on the 21st day (99.28 ± 0.44%). In conclusion, both plants and their combination showed promising results for burn healing.

Extracts of Brocchia cinerea (Delile) Vis Exhibit In Vivo Wound Healing, Anti-Inflammatory and Analgesic Activities, and Other In Vitro Therapeutic Effects

“…To our knowledge, this is the first study to report on the healing activity of extracts of B. cinerea, which have not been reported among the 12 species belonging to the Asteraceae family—species that are part of medicinal plants widely used in the treatment of wounds in the Mediterranean region [ 54 ]. An evaluation of the healing activity of a plant species belonging to the asteraceae family and using the same burn model with an extract of Ditrichia viscosa shows a remarkable wound contraction (99.28%) after 21 days of treatment [ 27 ]. The healing efficacies of some species of asteraceae can be attributed to their antibacterial effects, which provide protection of the microenvironment and damaged wound tissue against pathogenic bacterial strains [ 55 ].…”

“…Wavelengths used for detection ranged from 200 to 800 nm. The elution gradient was: at 0 min (5% X, 95% Y); at 6.25 min (30% X, 70% Y); at 12.5 min (35% X, 65% Y); at 16.25 min (70% X, 30% Y); at 17.5 min (100% X, 0% Y); at 18.75 min (5% X, 95% Y) [ 27 ].…”

Phenolic Composition, Wound Healing, Antinociceptive, and Anticancer Effects of Caralluma europaea Extracts

“…Ointments were kept at 4 °C in airtight containers. The positive and negative controls were Madecassol ® 1% and Vaseline ® [ 55 ]. Madecassol ® 1% is a hydrocotyl ointment (1%), derived from the dry extract of Centella asiatica , used in the local treatment of skin wounds [ 56 ].…”

“…The burned areas of all animals groups were photographed using a ruler as a scale. At the end of the study, images of each day were evaluated using ImageJ software to determine the wound closure percentage [ 55 ].…”

Histopathological Evaluation of the Healing Process of Standardized Skin Burns in Rabbits: Assessment of a Natural Product with Honey and Essential Oils

“…The majority of these cases occur in low- and middle-income countries in African and South-East Asia regions [ 3 ]. Additionally, skin burns are one of the complicated affections [ 4 ] which recorded in different animals [ 5 , 6 , 7 , 8 ]. However, to our knowledge, there is no documented data to measure the number of skin affections in animals, neither locally nor globally.…”