Off-line comprehensive two-dimensional high-performance liquid chromatography system with size exclusion column and reverse phase column for separation of complex traditional Chinese medicine Qingkailing injection

Ma, Sai; Chen, Lingxin; Luo, Guangsheng; Ren, Kangning; Wu, Juanfang; Wang, Yiming

doi:10.1016/j.chroma.2006.06.021

Cited by 36 publications

(16 citation statements)

References 15 publications

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“…To date, only a few reports are available [13 -20] on the application of 2-D separation systems coupled with MS in natural products analysis. Ma et al [13] developed an offline 2D-LC system with a size exclusion column and an RP column for the separation of a traditional Chinese formula Qingkailing. The result showed that the total peak capacity of this system could reach 1134 and the qualitative analysis of seven chemical components of the Qingkailing was accomplished by using this system.…”

Section: Introductionmentioning

confidence: 99%

Two‐dimensional liquid chromatography coupled with mass spectrometry for the analysis of Lobelia chinensis Lour. using an ESI/APCI multimode ion source

Zhou

Wang

et al. 2008

J of Separation Science

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A comprehensive approach for the separation and identification of components in a traditional Chinese medicine Lobelia chinensis Lour. was developed using 2D-HPLC coupled with an online photodiode array (PDA) detector and a mass spectrometer. The extract of L. chinensis Lour. was separated on a CN column in the first-dimensional HPLC, and then each of the collected fractions was further separated on an ODS column followed by an online PDA detector. After separation in the two different chromatographic modes, the eluents were delivered to a quadrupole mass spectrometer equipped with a multimode ion source of an ESI and an atmospheric pressure chemical ionization (ESI/APCI). At least 536 components in L. chinensis Lour. extract were detected and 6 of them were identified as apigenin 7-O-rutinoside, luteolin, lobetyolinin, lobetyolin, diosmin, and linarin, respectively, according to their UV spectrum and mass spectrum. The results demonstrated the powerful resolution, high peak capacity, as well as the identification capability of the 2D-HPLC combined with PDA and ESI/APCI-MS for the analyses of complex samples.

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Section: Introductionmentioning

confidence: 99%

Two‐dimensional liquid chromatography coupled with mass spectrometry for the analysis of Lobelia chinensis Lour. using an ESI/APCI multimode ion source

Zhou

Wang

et al. 2008

J of Separation Science

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“…Up to now, more and more applications of UPLC in analysis of TCMs have been reported [62][63][64]. Another promising development in HPLC research is the introduction of comprehensive two-dimensional LC (2D HPLC), resulting in much higher capacities, resolution and separation efficiency than one-dimensional LC [65][66][67][68][69][70]. Ma combined size exclusion chromatography and reverse phase liquid chromatography to analyze a complex prescription, Qingkailing injection.…”

Section: Hplc and Hyphenated Techniquesmentioning

confidence: 99%

“…Three-dimensional chromatogram of Qingkailing injection after twodimensional chromatography, which was drawn by MATLAB 6.5 software with low baseline (Peak assignments are same as described in Ref. [70]. Reprint with kind permission from Elsevier).…”

Section: Hplc and Hyphenated Techniquesmentioning

confidence: 99%

Recent analytical approaches in quality control of traditional Chinese medicines—A review

Jiang

David

et al. 2010

Analytica Chimica Acta

422

268

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“…Besides proteomic applications comprehensive 2-D chromatographic system combining SEC and RPLC was suggested also for the separation of the complex traditional Chinese medicine Qingkailing [127]. In this case, SEC column (300 mm long, 8 mm id) was followed by Hypersil ODS C18 RP column (100 mm long, 4.6 mm id) with the effluent injected from the first column automatically for the second dimension.…”

Section: Sec-rplcmentioning

confidence: 99%

Multidimensional liquid phase separations for mass spectrometry

Tomáš

Klepárnı́k

Foret

2008

J of Separation Science

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Large part of the current research in biology, medicine, and biotechnology depends on the analysis of DNA (genomics), proteins (proteomics), or metabolites (metabolomics). The advances in biotechnology also command development of adequate analytical instrumentation capable to analyze minute amounts of samples. The analysis of the content of single cells may serve as an example of ultimate analytical applications. Most of the separation techniques have been developed in the last three decades and alternative approaches are being investigated. At present, the main protocols for analyses of complex mixtures include 2-DE (IEF) followed by electrophoresis in SDS polyacrylamide gel (SDS-PAGE) and chromatographic techniques. Information-rich techniques such as MS and NMR are essential for the identification and structure analysis of the analyzed compounds. High resolution separation of the individual sample components is often a prerequisite for success. High resolution proteomic analysis in the majority of laboratories still relies on the time consuming and laborious offline methods. This review highlights some of the important aspects of 2-D separations including microfluidics.

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Off-line comprehensive two-dimensional high-performance liquid chromatography system with size exclusion column and reverse phase column for separation of complex traditional Chinese medicine Qingkailing injection

Cited by 36 publications

References 15 publications

Two‐dimensional liquid chromatography coupled with mass spectrometry for the analysis of Lobelia chinensis Lour. using an ESI/APCI multimode ion source

Two‐dimensional liquid chromatography coupled with mass spectrometry for the analysis of Lobelia chinensis Lour. using an ESI/APCI multimode ion source

Recent analytical approaches in quality control of traditional Chinese medicines—A review

Multidimensional liquid phase separations for mass spectrometry

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