Off‐line capillary electrophoresis/fully automated nanoelectrospray chip quadrupole time‐of‐flight mass spectrometry and tandem mass spectrometry for glycoconjugate analysis

Bîndilă, Laura; Almeida, Reinaldo; Sterling, Alistair; Allen, Mark; Peter‐Katalinić, Jasna; Zamfir, Alina D.

doi:10.1002/jms.707

Cited by 34 publications

(45 citation statements)

References 42 publications

(28 reference statements)

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“…2, two relevant ring cleavage ions at m/z 350.04 and at m/z 306.08 corresponding to 0,4 A 2 and 0,4 A 2 -CO 2 are detected. According to our previous studies 29,30 these cleavages are diagnostic for the NeuAca2-6 linkage.…”

Section: Resultsmentioning

confidence: 73%

Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

et al. 2005

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A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucidation of glycoconjugates originating from human body fluids and tissues such as a glycopeptide mixture from normal human urine and an isolated and purified GT1 ganglioside fraction from normal adult human brain. The optimization procedure required by each glycoconjugate category is described and the advantages of the system in terms of flexibility and adaptability to QTOF MS, stability of the ESI MS signal, carbohydrate ionization and sequencing, sensitivity, speed of analysis and sample consumption are discussed.

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Section: Resultsmentioning

confidence: 73%

Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

et al. 2005

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“…Concerning the analysis of peptides by CE-MS, a high variety of mass analyzers have been used, mainly single quadrupole (Q), triple quadrupole (QqQ), IT, and TOF, as well as other more expensive systems as quadrupole-TOF MS [38][39][40], Hadamard transform TOF MS [41], Fourier transform ion cyclotron resonance MS (FTICR-MS) [17,[42][43][44], etc. However, the employment of these advanced MS analyzers is not so common, obviously due to their high price and more difficult operation routine.…”

Section: Ms Analyzersmentioning

confidence: 99%

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

2008

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In the present work, an exhaustive review of the main developments and applications of CE-MS for peptide analysis is given. This review includes the use of different CE separation modes, MS analyzers, capillary coatings, preconcentration techniques, on-chip applications as well as other different multidimensional strategies for peptide analysis. Key applications are critically discussed and relevant works published from January 2000 to May 2007 are summarized including information concerning the type of sample, CE-MS parameters as well as some figures of merit of the different CE-MS procedures developed for peptide analysis and peptidomics.

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“…Among the postseparation treatments, increasing the sample concentration by solvent evaporation is the most frequent and time-consuming one. These two inherent problems associated to the off-line CE-MS coupling could be to a significant extent overcome by automated chip-based negative ion ESI-QTOF-MS in off-line conjunction with CE [55]. In this study, for testing the compatibility of the off-line CE coupling to the (2)nano ESIchip NanoMate/QTOF-MS for ionization and detection in a given CE buffer system, a multicomponent mixture of O-glycosylated amino acids and peptides from urine of patients suffering from Schindler disease was dissolved in 0.1 M formic acid/ammonia pH 2.8 to a concentration of 5 pmol/mL and infused automatically into MS. A constant and stable spray over 10 min analysis time and high ionic current indicated the compatibility of this buffer system with automatic chip-based ESI and that the analyte molecules could be efficiently ionized.…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

“…Nomenclature for assignment of fragment ions is according to [61,62]. Reproduced from [55], with permission.…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

Chip electrospray mass spectrometry for carbohydrate analysis

Zamfir¹,

Bîndilă²,

Lion³

et al. 2005

Electrophoresis

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Currently two types of chip systems are used in conjunction with MS: out-of-plane devices, where hundreds of nozzles, nanospray emitters are integrated onto a single silicon substrate from which electrospray is established perpendicular to the substrate, and planar microchips, embedding a microchannel at the end of which electrospray is generated in-plane, on the edge of the microchip. In the last two years, carbohydrate research greatly benefited from the introduction and implementation of the chip-based MS. In two laboratories the advantages of the chip electrospray in terms of ionization efficiency, sensitivity, reproducibility, quality of data in combination with high mass accuracy, and resolution of detection were systematically explored for several carbohydrate classes: O-and N-glycopeptides, oligosaccharides, gangliosides and glycoprotein-derived O-and N-glycans, and glycopeptides. The current state-of-the-art in interfacing the chip electrospray devices to high-performance MS for carbohydrate analysis, and the particular requirements for method optimization in both positive and negative ion modes are reviewed here. The recent applications of these miniaturized devices and their general potential for glycomic-based surveys are highlighted.

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Off‐line capillary electrophoresis/fully automated nanoelectrospray chip quadrupole time‐of‐flight mass spectrometry and tandem mass spectrometry for glycoconjugate analysis

Cited by 34 publications

References 42 publications

Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

Chip electrospray mass spectrometry for carbohydrate analysis

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