Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

Zamfir, Alina D.; Lion, Niels; Vukelić, Željka; Bîndilă, Laura; Rossier, Joël S.; Peter‐Katalinić, Jasna

doi:10.1039/b413282c

Cited by 40 publications

(54 citation statements)

References 35 publications

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“…The ESI plume is clearly visible demonstrating the instant initiation of the electrospray. Under the same ESI-QTOF-MS conditions, the reported [27] in-run reproducibility in terms of sensitivity, spray stability, number of detected components/fragments, ion intensity, and charge state was almost 100%, while the day-to-day reproducibility was 95-100%.…”

Section: Coupling Of the Thin Chip Microsprayer System To High-performentioning

confidence: 75%

“…The voltage on the microelectrode was applied by using a conductive wire with one terminal connected to the chip electrode and the other fixed on the ESI high-voltage plate of the QTOF-MS instrument. Under the formed high electric field, the fluid was electrokinetically driven through the chip microchannel from its inlet-hole toward the outlet-edge where the electrospray process occurred [27]. In the case of QTOF-MS, the ESI source is configured in Z geometry by the orthogonal alignment of the sampling capillary and MS counterelectrode.…”

Section: Coupling Of the Thin Chip Microsprayer System To High-performentioning

confidence: 99%

“…The thin polymer chip was coupled to a QTOF-MS (Micromass, UK) with Z-spray ion source geometry [27]. For sample loading, a microvial reservoir was pasted over the inlet-hole of the microchannel and the whole chip/ reservoir assembly was mounted onto the mass spectrometer.…”

Section: Coupling Of the Thin Chip Microsprayer System To High-performentioning

confidence: 99%

“…The thin chip microsprayer/QTOF-MS coupling described above was optimized in the negative ion mode for determination of O-GalNAc-Ser/Thr expression in the normal human urine [27]. A mixture of O-GalNAc glycosylated amino acids and peptides was dissolved in pure methanol to a concentration of 5 pmol/mL and an aliquot of 10 mL was dispensed into the reservoir pasted over the inlet of the chip microchannel.…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

“…For screening and sequencing of carbohydrates, recently, a disposable polymer microchip with integrated microchannels and electrodes was coupled to both a quadrupole TOF (QTOF) and an FTICR mass spectrometer [27,28].…”

Section: Coupling Of the Thin Chip Microsprayer System To High-performentioning

confidence: 99%

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Chip electrospray mass spectrometry for carbohydrate analysis

Zamfir¹,

Bîndilă²,

Lion³

et al. 2005

Electrophoresis

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Currently two types of chip systems are used in conjunction with MS: out-of-plane devices, where hundreds of nozzles, nanospray emitters are integrated onto a single silicon substrate from which electrospray is established perpendicular to the substrate, and planar microchips, embedding a microchannel at the end of which electrospray is generated in-plane, on the edge of the microchip. In the last two years, carbohydrate research greatly benefited from the introduction and implementation of the chip-based MS. In two laboratories the advantages of the chip electrospray in terms of ionization efficiency, sensitivity, reproducibility, quality of data in combination with high mass accuracy, and resolution of detection were systematically explored for several carbohydrate classes: O-and N-glycopeptides, oligosaccharides, gangliosides and glycoprotein-derived O-and N-glycans, and glycopeptides. The current state-of-the-art in interfacing the chip electrospray devices to high-performance MS for carbohydrate analysis, and the particular requirements for method optimization in both positive and negative ion modes are reviewed here. The recent applications of these miniaturized devices and their general potential for glycomic-based surveys are highlighted.

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Section: Coupling Of the Thin Chip Microsprayer System To High-performentioning

confidence: 75%

Section: Coupling Of the Thin Chip Microsprayer System To High-performentioning

confidence: 99%

Section: Coupling Of the Thin Chip Microsprayer System To High-performentioning

confidence: 99%

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

Section: Coupling Of the Thin Chip Microsprayer System To High-performentioning

confidence: 99%

See 3 more Smart Citations

Chip electrospray mass spectrometry for carbohydrate analysis

Zamfir¹,

Bîndilă²,

Lion³

et al. 2005

Electrophoresis

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Sheathless reverse‐polarity capillary electrophoresis‐electrospray‐mass spectrometry for analysis of underivatized glycoconjugates

2005

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We report on the development of a novel methodology to extend the limits of capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) general applicability. A sheathless on-line CE-ESI-MS setup was optimized on standard monosaccharide mixture to operate in reverse polarity and negative ion mode for MS detection without pressure to assist the sample migration, coating of the capillaries, and/or sample derivatization. This approach was further applied for screening of a complex glycopeptide mixture obtained from the urine of a patient diagnosed with N-acetylhexosaminidase deficiency, known as Schindler's disease. The potential of this methodology in terms of high sensitivity, separation efficiency, resolution, and reproducibility is demonstrated. In combination with the high quality of MS data, a new, significantly improved insight into the sample heterogeneity is possible.

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Recent advances in the MS analysis of glycoproteins: Capillary and microfluidic workflows

et al. 2010

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Recent developments in bioanalytical instrumentation, mass spectrometry (MS) detection and computational data analysis approaches have provided researchers with capabilities for interrogating the complex cellular glycoproteome, to help gain a better insight into the cellular and physiological processes that are associated with a disease, and to facilitate the efforts centered on identifying disease-specific biomarkers. This review describes the progress achieved in the characterization of protein glycosylation by using advanced capillary and microfluidic-MS technologies. The major steps involved in large-scale glycoproteomic analysis approaches are discussed, with special emphasis given to workflows that have evolved around complex MS detection functions. In addition, quantitative analysis strategies are assessed, and the bioinformatics aspects of glycoproteomic data processing are summarized. The developments in commercial and custom fabricated microfluidic front-end platforms to ESI- and MALDI-MS instrumentation, for addressing major challenges in carbohydrate analysis such as sensitivity, throughput and ability to perform structural characterization, are further evaluated and illustrated with relevant examples.

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Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

Cited by 40 publications

References 35 publications

Chip electrospray mass spectrometry for carbohydrate analysis

Chip electrospray mass spectrometry for carbohydrate analysis

Sheathless reverse‐polarity capillary electrophoresis‐electrospray‐mass spectrometry for analysis of underivatized glycoconjugates

Recent advances in the MS analysis of glycoproteins: Capillary and microfluidic workflows

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