Oestrogen and progesterone receptors and their relationship to histological grades of epithelial tumours of the magnum region of the oviduct of the domestic fowl

Anjum, A. D.; Payne, L. N.; Appleby, E. C.

doi:10.1016/0021-9975(89)90105-9

Cited by 14 publications

(12 citation statements)

References 15 publications

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“…Therefore, such hormones were thought to play important roles in the development of genital organ tumours [5,20,21] . The dorsal oviductal ligament suspends the oviduct and continues ventrally as the fanlike oviduct VL the free edge of which is reinforced by smooth muscle.…”

Section: Discussionmentioning

confidence: 99%

Leiomyomas of Oviduct and Its Ventral Ligament of Hens: Immunohistochemical Evaluation and the Plasma Concentration Levels of 17 β-Oestradiol and Progesterone

Tunca¹,

Özkul²,

Salmanoğlu³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryLeiomyoma's of the oviduct and its ventral ligament (VL) are common tumours of the domestic fowl which develop after the end of the first laying season. In the present study, macroscopic, microscopic, and immunohistochemical features of the leiomyomas of the reproductive tract and their prevalence were investigated in commercial laying hens. In 60-90 weeks old commercial laying hens, toward the end of the first laying period, the incidence of genital tract leiomyomas were determined as 10.43%. Of these, 95.81% were developed from oviduct VL and 4.19% were oviduct leiomyomas. These tumours were firm, round to oval and microscopically well-circumscribed, consisting of monomorphic spindle cells. In immunohistochemical examination with α-smooth muscle actin, desmin and vimentin, all tumours were found positive with these markers. To investigate the aetiological importance for tumourogenesis, the plasma concentrations of 17 β-oestradiol and progesterone levels were determined. Concentrations of 17 β-oestradiol and progesterone were higher in hens with tumours than that of non-tumour control animals. The results suggested that there was an association between the levels of 17 β-oestradiol and progesterone and the leiomyomas of genital tract in hens. Keywords: Leiomyomas of hens' oviduct, Incidence, Immunohistochemical evaluation, 17 β-oestradiol and Progesteron levels Yumurtacı Tavuklarda Ovidukt Ventral Ligamenti Leiomyomları:İmmunohistokimyasal İnceleme ve Plazma 17 β-Östradiol ve Progesteron Düzeyleri ÖzetOvidukt ve ovidukt ventral ligamenti (VL)nin leiomiyomları, ilk yumurtlama periyodunu tamamlayan yumurtacı tavuklarda oldukça sık karşılaşılan tümörlerdir. Çalışmada, ticari olarak yetiştirilen yumurtacı tavukların reprodüktif sistemlerinde gelişen leiomiyomların sıklığı ve bu tümörlerin makroskobik, mikroskobik ve immunohistokimyasal özellikleri değerlendirildi. İlk yumurtalama periyodunu tamamlamış, 60-90 haftalık yaşta, ticari yumurtacı tavuklarda, reprodüktif sistem leiomiyomlarının insidensi %10.43 olarak saptandı. Saptanan leiomiyomların %95.81'inin ovidukttun ventral ligamentinde, %4.19'un oviduktta yerleştiği belirlendi. Makroskobik olarak sert kıvamlı ve yuvarlak-oval şekilli olan bu tümörlerin mikroskobik incelemesinde iyi sınırlandırılmış mekik şeklindeki hücrelerden oluştuğu belirlendi. İmmunohistokimyasal incelemede, tüm tümörlerin α-smooth muscle actin, desmin ve vimentin primer antikorları ile pozitif reaksiyon verdikleri belirlendi. 17 β-östradiol ve progesteronun tümörogenezisteki etiyolojik öneminin değerlendirilmesi amacıyla plazma konsantrasyonları belirlendi. Tümör tespit edilen yumurtacı tavukların plazma 17 β-östradiol ve progesteron düzeyleri kontrol gruplarındaki yumurtacı tavuklardan anlamlı derecede yüksekti. Elde edilen bu sonuçlar ile, yumurtacı tavukların reprodüktif sistemlerinde sıklıkla ortaya çıkan leiomiyomların gelişiminin, plazma 17 β-östradiol ve progesteron düzeyleri ile ilişkili olduğu görüldü.

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Section: Discussionmentioning

confidence: 99%

Leiomyomas of Oviduct and Its Ventral Ligament of Hens: Immunohistochemical Evaluation and the Plasma Concentration Levels of 17 β-Oestradiol and Progesterone

Tunca¹,

Özkul²,

Salmanoğlu³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…A remarkable example of the use of an animal model as a research tool is the utilization of the domestic hen (Gallus gallus domesticus) to study the molecular mechanisms involved in the development of ovarian carcinoma in humans [1][2][3][4][5][6]. Similar to what occurs in ovarian carcinoma, leiomyoma-like masses also occur on the oviduct of commercial strains of chickens [7,8]. These spontaneous leiomyomas in hens share several histologic features with human uterine leiomyomas [9].…”

Section: Introductionmentioning

confidence: 99%

Validation of the Aging Hen (Gallus gallus domesticus) as an Animal Model for Uterine Leiomyomas1

Machado

Bahr

Hales

et al. 2012

Biology of Reproduction

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Uterine leiomyomas, or fibroids, are the most frequent gynecological tumors in premenopausal women with as many as 65% of women becoming clinically symptomatic. Uterine fibroids are benign myometrial tumors that produce large quantities of extracellular matrix proteins. Despite its high morbidity, the molecular basis underlying the development of uterine leiomyomas is not well understood. Domestic hens of Gallus gallus domesticus develop oviductal leiomyomas similar to those found in humans. We investigated the natural history of chicken leiomyomas, in vivo expression of protein biomarkers, and in vitro expression of ovarian steroid receptors. Based on the analysis of 263 hens, tumor prevalence, tumor number per hen, and tumor size increased as the hens aged. Immunohistochemistry for alpha-smooth muscle actin (SMA) and desmin confirmed the smooth muscle phenotype of the chicken leiomyomas. Intense collagen expression was detected in these oviductal leiomyomas by Mason's trichrome, and the tumors also showed increased expression of TGFB3 and collagen type I mRNAs. Consistent with human leiomyomas, chicken fibroids displayed increased BCL2 and estrogen (E) and progesterone (P) receptor expression. Chicken leiomyomas were dissociated for in vitro culture. Cells from explants were positive for SMA, desmin, and E and P receptors until the fourth passage. These cells also displayed a response similar to human cells when challenged with halofuginone, an antifibrotic agent. Our findings indicate that the chicken is an excellent complementary model for studies involving the pathophysiology of human uterine leiomyomas.

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“…Medical treatment involves gonadotropin-releasing hormone (GnRH) agonists, GnRH-antagonists, and oral contraceptive pills; whereas surgical approaches are myomectomy, hysterectomy, endometrial ablation, or uterine artery occlusion (1). Smooth muscle tumors are also among the most common neoplasm in the avian species, with prevalence varying up to 60% (6,7). Particularly in the quail, Foster et al (8) reported that frequency of neoplasm ranged from 38.7 to 80%.…”

Section: Introductionmentioning

confidence: 99%

Genistein Suppresses Spontaneous Oviduct Tumorigenesis in Quail

Şahin

Akdemir

Tuzcu

et al. 2009

Nutrition and Cancer

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Spontaneous leiomyomas of the oviduct are common tumors of the Japanese quail (Coturnix coturnix japonica) and laying hens. This makes it a good animal model for screening potential agents for testing in the prevention and treatment of human myoma uteri. Genistein has been shown to inhibit the growth of various cancer cells. We investigated the effects of genistein supplementation on the development of fibroid tumors in the oviduct, serum oxidative stress markers [malondialdehyde (MDA), 8-isoprostane, 4-hydroxyalkenal (HAE), 8-hydroxy-2' -deoxyguanosine (8-OHdG) levels], soy isoflavone levels, and tissue biomarkers [Connexin 43 (Cx43), Bcl-2, and Bax and heat shock protein 70 (Hsp70) expression] in Japanese quail. One hundred and fifty quail (12 mo old) were assigned to 3 experimental groups as 5 replicates of pens containing 10 birds in each. Birds were fed either a basal diet or the basal diet supplemented with 400 mg or 800 mg of genistein/kg of diet. The animals were sacrificed after 315 days, and the tumors were identified. Genistein supplementation significantly decreased the incidence of fibroid tumors as compared to control birds (P = 0.04). The tumors in genistein-fed birds were smaller than those found in control birds (P = 0.02). Serum MDA, 8-isoprostane, and HAE levels were lower in treatment groups than in control group (MDA: 2.01 vs. 0.82; 8-isoprostane: 135 vs. 101; HAE: 1.45 vs. 0.73; P

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Oestrogen and progesterone receptors and their relationship to histological grades of epithelial tumours of the magnum region of the oviduct of the domestic fowl

Cited by 14 publications

References 15 publications

Leiomyomas of Oviduct and Its Ventral Ligament of Hens: Immunohistochemical Evaluation and the Plasma Concentration Levels of 17 β-Oestradiol and Progesterone

Leiomyomas of Oviduct and Its Ventral Ligament of Hens: Immunohistochemical Evaluation and the Plasma Concentration Levels of 17 β-Oestradiol and Progesterone

Validation of the Aging Hen (Gallus gallus domesticus) as an Animal Model for Uterine Leiomyomas1

Genistein Suppresses Spontaneous Oviduct Tumorigenesis in Quail

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