Leiomyomas of Oviduct and Its Ventral Ligament of Hens: Immunohistochemical Evaluation and the Plasma Concentration Levels of 17 β-Oestradiol and Progesterone

Abstract

SummaryLeiomyoma's of the oviduct and its ventral ligament (VL) are common tumours of the domestic fowl which develop after the end of the first laying season. In the present study, macroscopic, microscopic, and immunohistochemical features of the leiomyomas of the reproductive tract and their prevalence were investigated in commercial laying hens. In 60-90 weeks old commercial laying hens, toward the end of the first laying period, the incidence of genital tract leiomyomas were determined as 10.43%. Of these, 95.81% were developed from oviduct VL and 4.19% were oviduct leiomyomas. These tumours were firm, round to oval and microscopically well-circumscribed, consisting of monomorphic spindle cells. In immunohistochemical examination with α-smooth muscle actin, desmin and vimentin, all tumours were found positive with these markers. To investigate the aetiological importance for tumourogenesis, the plasma concentrations of 17 β-oestradiol and progesterone levels were determined. Concentrations of 17 β-oestradiol and progesterone were higher in hens with tumours than that of non-tumour control animals. The results suggested that there was an association between the levels of 17 β-oestradiol and progesterone and the leiomyomas of genital tract in hens. Keywords: Leiomyomas of hens' oviduct, Incidence, Immunohistochemical evaluation, 17 β-oestradiol and Progesteron levels Yumurtacı Tavuklarda Ovidukt Ventral Ligamenti Leiomyomları:İmmunohistokimyasal İnceleme ve Plazma 17 β-Östradiol ve Progesteron Düzeyleri ÖzetOvidukt ve ovidukt ventral ligamenti (VL)nin leiomiyomları, ilk yumurtlama periyodunu tamamlayan yumurtacı tavuklarda oldukça sık karşılaşılan tümörlerdir. Çalışmada, ticari olarak yetiştirilen yumurtacı tavukların reprodüktif sistemlerinde gelişen leiomiyomların sıklığı ve bu tümörlerin makroskobik, mikroskobik ve immunohistokimyasal özellikleri değerlendirildi. İlk yumurtalama periyodunu tamamlamış, 60-90 haftalık yaşta, ticari yumurtacı tavuklarda, reprodüktif sistem leiomiyomlarının insidensi %10.43 olarak saptandı. Saptanan leiomiyomların %95.81'inin ovidukttun ventral ligamentinde, %4.19'un oviduktta yerleştiği belirlendi. Makroskobik olarak sert kıvamlı ve yuvarlak-oval şekilli olan bu tümörlerin mikroskobik incelemesinde iyi sınırlandırılmış mekik şeklindeki hücrelerden oluştuğu belirlendi. İmmunohistokimyasal incelemede, tüm tümörlerin α-smooth muscle actin, desmin ve vimentin primer antikorları ile pozitif reaksiyon verdikleri belirlendi. 17 β-östradiol ve progesteronun tümörogenezisteki etiyolojik öneminin değerlendirilmesi amacıyla plazma konsantrasyonları belirlendi. Tümör tespit edilen yumurtacı tavukların plazma 17 β-östradiol ve progesteron düzeyleri kontrol gruplarındaki yumurtacı tavuklardan anlamlı derecede yüksekti. Elde edilen bu sonuçlar ile, yumurtacı tavukların reprodüktif sistemlerinde sıklıkla ortaya çıkan leiomiyomların gelişiminin, plazma 17 β-östradiol ve progesteron düzeyleri ile ilişkili olduğu görüldü.

Myxoid leiomyosarcoma of the oviduct and uterus in a Cockatiel (Nymphicus hollandicus)

“…Neoplasms originating from the oviduct are less common compared to ovarian tumours (Echols & Speer, 2022 ). Adenoma, adenomatous hyperplasia (Echols & Speer, 2022 ), adenocarcinoma (Carleton & Garner, 2002 ; Schmidt et al., 2015 ), hemangiosarcoma (Rosen, 2012 ), lymphoid leukosis (Greenacre, 2015 ), carcinomatosis (Rosen, 2012 ), granulosa cell tumour (Robat et al., 2017 ), dysgerminoma (Strunk et al., 2011 ), leiomyoma (Anjum et al., 1988 ; Tunca et al., 2014 ) and rarely LMS (Antinoff et al., 1997 ) have been reported in the reproductive tract of female birds. Conventional LMS has also been reported in the skin and subcutis (Nemeth et al., 2016 ; Timurkaan et al., 2016 ), large intestine (Cardoso & Levy, 2014 ), jejunum (Steinberg, 1988 ) and anterior limb (Bonel et al., 2019 ) of pet birds.…”

Survey of the Clinical, Cytological, and Histopathological Features Associated with Neoplasms in Captive Avian Species in Melbourne, Australia

“…2,65 These tumors are usually slow growing and are often incidental findings at necropsy in commercial chickens. 85 Spontaneous leiomyomas of the oviduct and its ventral ligament are common tumors of chickens that typically develop after the end of the first laying season, 85,86 with a reported incidence of 10.4% in 60-to 90-week-old laying hens. 85 It is noteworthy that despite chickens being the most numerous species in our study population, we identified only a single case of leiomyoma in a chicken, and the tumor affected the intestines rather than the oviduct.…”

“…85 Spontaneous leiomyomas of the oviduct and its ventral ligament are common tumors of chickens that typically develop after the end of the first laying season, 85,86 with a reported incidence of 10.4% in 60- to 90-week-old laying hens. 85 It is noteworthy that despite chickens being the most numerous species in our study population, we identified only a single case of leiomyoma in a chicken, and the tumor affected the intestines rather than the oviduct. In pheasants (family Phasianidae), cecal leiomyomas have been reported to occur in conjunction with chronic Heterakis spp.…”