Oestradiol Assays: Applications and Guidelines for the Provision of a Clinical Biochemistry Service

Ratcliffe, W. A.; Carter, G D; Dowsett, Mitchell; Hillier, Stephen G.; Middle, Jonathan; Reed, Michael J.

doi:10.1177/000456328802500502

Cited by 54 publications

(24 citation statements)

References 38 publications

(1 reference statement)

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“…Blood sample centrifuged and serum separation was used for the analysis of biochemical parameters given below-1. Serum Estradiol, (7) Testosterone, (8) Insulin, (9) SHBG, (10) DHEA (11) In the categorised groups, mean BMI and other basic parameters were in normal range in categorised groups. Although, mean of sex hormone profile found to be different in both the groups, but were not statistically significant.…”

Section: Exclusion Criteriamentioning

confidence: 94%

A Study of Association of Sex Hormones With Insulin Resistance and Obesity for Diabetes Mellitus Risk in Adult Women

Suganthy¹,

Mohanty²,

Jeyakumar³

et al. 2017

jemds

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BACKGROUNDSex hormones have a great impact on energy metabolism, body composition, vascular function and inflammatory responses and insulin resistance. Ovarian hormones influence insulin sensitivity across the lifespan of women. DM prevalence also affects women's health across the life stages causing the second highest mortality in South Asians. DM has polyfactorial aetiological differences between males and females. There is need of search of sex/gender specific risk factors of DM in middle aged women. Therefore, this study is undertaken to analyse the association of sex hormones with insulin resistance, obesity and lipid profile thereby to assess the DM risk.

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Section: Exclusion Criteriamentioning

confidence: 94%

A Study of Association of Sex Hormones With Insulin Resistance and Obesity for Diabetes Mellitus Risk in Adult Women

Suganthy¹,

Mohanty²,

Jeyakumar³

et al. 2017

jemds

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“…-Determination of hormones (estradiol, progesterone and follicle stimulating hormone) by immunologic methods (Elisa technique) using commercial kits purchased from DRG diagnostic Germany [12][13][14].…”

Section: Methodsmentioning

confidence: 99%

Reproductive outcome, hormone levels and liver enzymes in agricultural female workers

Ibrahim

Amer

Tahlawy

et al. 2011

Journal of Advanced Research

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The study aimed to explore the effects of exposure to pesticides on the reproductive health and liver function of females participating in agricultural work. Blood samples were obtained from the thirty-five females (out of one hundred females of reproductive age) who were willing to donate blood. Fifty females matched for age and socio economic status were recruited as a control group to compare levels of hormones (estradiol, progesterone and follicle stimulating hormone), liver enzymes alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Results showed that 53% of the females examined in this village were illiterate. Agricultural work represented 42% of the occupations encountered by females in the study. Females who helped in farming operations showed a higher incidence of adverse pregnancy outcome such as abortions and stillbirths compared to those who did not share in agricultural work. There was a significant elevation in estradiol and progesterone levels among female agricultural workers and controls. There was also elevation in serum liver enzymes (AST and ALT) in agricultural workers compared to the controls. However, there was a significant decrease in FSH level in females sharing in agricultural activities compared to the controls. It could be concluded that participation of females in agricultural activities with potential exposure to pesticides endangers their reproductive health and liver functions. Rural areas in Egypt require more attention to increase the percentage of literacy among females and raise their health awareness.

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“…The level of E2 has been shown to correlate with the diameter of the leading follicle in spontaneous and hMG-stimulated cycles [6]. However, there is no general agreement on the E2 increment in serum which could be expressed from each developing follicle; thus figures of 0.73-1.42 nmol/1 have been men tioned [7,8], When more than one dominant follicle develops, a poor correlation between the ultrasonographic ovarian morphology and the plasma E2 level has been described [9], This corresponds to our study, where the follicular activity expressed as E2 concentration per folli cle exceeding 14 mm seemed to be lower in the superovulated cycles. But still the levels of E2/number of follicles depended on the method used.…”

Section: Estradiol Evaluated With Six Different Methodsmentioning

confidence: 99%

Results of Serial Measurement of Estradiol in Serum with Six Different Methods during Ovarian Stimulation

Mikkelsen¹,

Borggaard

Lebech³

1996

Gynecol Obstet Invest

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The aim of the study was to assess the values of serum estradiol (E₂) in women undergoing ovarian stimulation with five direct commercial assays (IMX, Amerlite, Spectria, Vidas, CA) and to compare them with the results of an E₂ direct method used in our laboratory. The study included 12 infertile women. Thirteen cycles were monitored daily during ovarian stimulation with transvaginal ultrasound and serum E₂. Large differences were found between the E₂ concentrations measured on the same sample with the different methods. In general the IMX and Amerlite assays gave the highest and CA the lowest results. The results of our own RIA and Spectria assay agreed more closely. The median ratio of the highest E₂ value (IMX assay) and the lowest E₂ value (CA value) was 1.75 (1.30–3.15). Values of E₂ measured in serum depend on the method used. It is not possible to apply criteria from other studies directly to one’s own patients. Close collaboration between the laboratories and the clinical staff is essential.

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Oestradiol Assays: Applications and Guidelines for the Provision of a Clinical Biochemistry Service

Cited by 54 publications

References 38 publications

A Study of Association of Sex Hormones With Insulin Resistance and Obesity for Diabetes Mellitus Risk in Adult Women

A Study of Association of Sex Hormones With Insulin Resistance and Obesity for Diabetes Mellitus Risk in Adult Women

Reproductive outcome, hormone levels and liver enzymes in agricultural female workers

Results of Serial Measurement of Estradiol in Serum with Six Different Methods during Ovarian Stimulation

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