Ocimum sanctum Linn. stimulate the expression of choline acetyltransferase on the human cerebral microvascular endothelial cells

Kusindarta, Dwi Liliek; Wihadmadyatami, Hevi; Haryanto, Aris

doi:10.14202/vetworld.2016.1348-1354

Cited by 15 publications

(19 citation statements)

References 37 publications

(51 reference statements)

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“…Kusindarta et al indicated that an ethanolic extract derived from leaves of Ocimum sanctum may stimulate and restores the expression of choline acetyltransferase in ageing human cerebral microvascular endothelial cells and could provide nerve protection and increased production of Ach may enhance the memory and cognitive ability [75]. Scientific studies reveal that the hydroalcoholic extract of Ocimum sanctum exhibits strong antioxidant activity against DPPH and hydroxyl radicals which may be due to the high amount of polyphenols and flavonoids.…”

Section: Ocimum Sanctummentioning

confidence: 99%

Herbal Medicines as Neuroprotective Agent: A Mechanistic Approach

Roy

Awasthi

2017

Int J Pharm Pharm Sci

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Neurodegeneration refers to a condition of neuronal death occurring as a result of progressive disease of long-term and is becoming a major health problem in the 21st century. Neurons degenerated are not replaced resulting in a cognitive loss, many neurodegenerative disorders, such as schizophrenia, depression, Alzheimer's disease (AD), dementia, cerebrovascular impairment, seizure disorders, head injury, parkinsonism. The common pathology of neurodegeneration includes deposition of misfolded proteins such as amyloid-β (Aβ) in Alzheimer's disease, α-synuclein in Parkinson's disease (PD), transactive response DNA-binding protein 43 (TDP-43) in dementia. Neuroprotection refers to the strategies and possible mechanisms that are able to protect the central nervous system (CNS) against neuronal injury and neurodegenerative disorders. The past decade has witnessed an intense interest in herbal plants having long-term health-promoting or medicinal qualities. Comprehensive research and discovery have demonstrated that natural products, medicinal herbs, plant extracts, and their metabolites, have great potential as the neuroprotective agent. Although the precise mechanisms of action of herbal drugs have yet to be determined, some of them have been shown to prevent formation of betaamyloid plaques, promote nerve growth, some inhibit acetylcholinesterase (AChE) enzyme and malondialdehyde (MDA) formation in brain while other exhibits antioxidant activity by increasing the level of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx). Thus the herbal plants can be a valuable source of the drug against neurodegenerative disorders which will require high-throughput screening. This review will highlight the role of herbal plants and their phytoconstituents against neurodegenerative diseases and other related disorders, focusing on their mechanism of action and therapeutic potential.

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Section: Ocimum Sanctummentioning

confidence: 99%

Herbal Medicines as Neuroprotective Agent: A Mechanistic Approach

Roy

Awasthi

2017

Int J Pharm Pharm Sci

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“…O. sanctum leaves prepared from Center for Research and Development of Medicinal Plants and Traditional Medicines, Ministry of Health in Tawangmangu, Central Java, Indonesia. Crude extracts and OSE prepared as previously described [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…The administration of O. sanctum helps to regulated neurotransmitter levels which play an essential role in neuronal function [ 18 ]. On the in vitro studies, OSE was able to maintain the stability of ChAT expression at human cerebral microvascular endothelial cells (HCMECs) mimic young age and restore ChAT expression at mimic old age on in vitro model [ 21 ]. Another evidence stated that OSE exhibits neuroprotective effects against H 2 O 2 induced neuronal cell damage in SH-SY5Y neuronal cells [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease

Hening

Wijayanti

et al. 2018

Vet World

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Aim:This study aimed to analyze the neuroprotective effect of Ocimum sanctum Linn. ethanolic extract (OSE) on human embryonic kidney-293 (HEK-293) cells as the in vitro model of neurodegenerative diseases.Materials and Methods:In this research, HEK-293 cells divided into five groups consisting of normal and healthy cells (NT), cells treated with Camptothecin 500 µM as the negative control, cells treated with trimethyltin 10 µM (TMT), cells treated with OSE 75 µg/ml, and cells pre-treated with OSE 75 µg/ml then induced by TMT 10 µM (OSE+TMT). MTT assay and phase contrast microscopy were applied to observe the cell viability quantitatively and morphological after Ocimum sanctum Linn extract treatment. Finally, the reverse transcription polymerase chain reaction was employed to study the expression of choline acetyltransferase (ChAT).Results:The MTT assay and phase contrast microscopy showed that OSE pre-treatment significantly increased the viability of TMT-induced apoptotic cells and maintained cell viability of the normal HEK-293 cells. Expression of ChAT markedly reduced on TMT treatment group, but OSE administration stabilized ChAT expression in TMT-induced HEK-293 cells.Conclusion:This present study proved that OSE administration has neuroprotective effect by increased HEK-293 cells viability and maintain ChAT expression.

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“…To solve the problem, some manipulation on the intrinsic extracellular matrix by using traditional herb such as Ocimum sanctum extract was already done. In the in vivo and in vitro model using human brain microvascular endothelial cells (HBMECs) which mimics bloodbrain barrier, the treatment of the extract may promote the cell proliferation on the hippocampus area and HBMECs in the condition upregulation of choline acetyltransferase (ChAT) enzyme [11,12]. In addition, there is also a chance to use nanometer-sized scaffolds in the presence of other substrates such as vascular endothelial growth factor or hyaluronic acid with laminin.…”

Section: Tissue Regenerationmentioning

confidence: 99%

The Role of Extracellular Matrix in Tissue Regeneration

Kusindarta¹,

Wihadmadyatami²

2018

Tissue Regeneration

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Extracellular matrix (ECM) is an extensive molecule network composed of three major components: protein, glycosaminoglycan, and glycoconjugate. ECM components, as well as cell adhesion receptors, interact with each other forming a complex network into which cells reside in all tissues and organs. Cell surface receptors transduce signals into cells from ECM, which regulate diverse cellular functions, such as survival, growth, proliferation, migration, differentiation, and some vital role in maintaining cells homeostasis. This chapter emphasizes the complex of ECM structure to provide a better understanding of its dynamic structural and functional characterization and multipotency. In this chapter the implications of ECM in tissue remodeling are mainly discuss on the neuronal regeneration and wound healing mechanism in the presence of human umbilical mesenchymal conditioned medium (HU-MSCM).

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Ocimum sanctum Linn. stimulate the expression of choline acetyltransferase on the human cerebral microvascular endothelial cells

Cited by 15 publications

References 37 publications

Herbal Medicines as Neuroprotective Agent: A Mechanistic Approach

Herbal Medicines as Neuroprotective Agent: A Mechanistic Approach

The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease

The Role of Extracellular Matrix in Tissue Regeneration

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