Ocimum gratissimum Essential Oil and Its Isolated Compounds (Eugenol and Myrcene) Reduce Neuropathic Pain in Mice

Paula-Freire, Lyvia Izaura Gomes; Molska, Graziella Rigueira; Andersen, Mônica Levy; Carlini, E. A.

doi:10.1055/s-0035-1558165

Cited by 20 publications

(19 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Myrcene has been identified as an analgesic in past studies including those of Traditional Medical System (TMS) approaches. As a component of Occimum oils, it has demonstrated anti-neuropathic pain effects in mice, working in combination with eugenol (a TRPA1 ligand) [65]. Analgesic oils from other settings contain Myrcene [66][67][68]69].…”

Section: Discussionmentioning

confidence: 99%

Myrcene and terpene regulation of TRPV1

et al. 2019

View full text Add to dashboard Cite

Nociceptive Transient Receptor Potential channels such as TRPV1 are targets for treating pain. Both antagonism and agonism of TRP channels can promote analgesia, through inactivation and chronic desensitization. Since plant-derived mixtures of cannabinoids and the Cannabis component myrcene have been suggested as pain therapeutics, we screened terpenes found in Cannabis for activity at TRPV1. We used inducible expression of TRPV1 to examine TRPV1-dependency of terpene-induced calcium flux responses. Terpenes contribute differentially to calcium fluxes via TRPV1 induced by Cannabis-mimetic cannabinoid/terpenoid mixtures. Myrcene dominates the TRPV1-mediated calcium responses seen with terpenoid mixtures. Myrcene-induced calcium influx is inhibited by the TRPV1 inhibitor capsazepine and Myrcene elicits TRPV1 currents in the wholecell patch-clamp configuration. TRPV1 currents are highly sensitive to internal calcium. When Myrcene currents are evoked, they are distinct from capsaicin responses on the basis of I max and their lack of shift to a pore-dilated state. Myrcene pre-application and residency at TRPV1 appears to negatively impact subsequent responses to TRPV1 ligands such as Cannabidiol, indicating allosteric modulation and possible competition by Myrcene. Molecular docking studies suggest a non-covalent interaction site for Myrcene in TRPV1 and identifies key residues that form partially overlapping Myrcene and Cannabidiol binding sites. We identify several non-Cannabis plantderived sources of Myrcene and other compounds targeting nociceptive TRPs using a data mining approach focused on analgesics suggested by non-Western Traditional Medical Systems. These data establish TRPV1 as a target of Myrcene and suggest the therapeutic potential of analgesic formulations containing Myrcene.

show abstract

Section: Discussionmentioning

confidence: 99%

Myrcene and terpene regulation of TRPV1

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Ocimum gratissimum L. is widely found in several geographical regions in South America and Africa [ 198 , 199 ] and still used as a medicinal plant with analgesic activity [ 198 ]. It contains several proanthocyanidins, which have been shown to exhibit significant antioxidant activity, and tannins, saponins, steroids, alkaloids, terpenoids, flavonoids, phenols, and cardiac glycosides [ 200 ].…”

Section: Species Of the Lamiaceae Family With Potential Analgesic/mentioning

confidence: 99%

Medicinal Plants of the Family Lamiaceae in Pain Therapy: A Review

Uritu

Mihai

Stanciu

et al. 2018

Pain Research and Management

218

132

View full text Add to dashboard Cite

Recently, numerous side effects of synthetic drugs have lead to using medicinal plants as a reliable source of new therapy. Pain is a global public health problem with a high impact on life quality and a huge economic implication, becoming one of the most important enemies in modern medicine. The medicinal use of plants as analgesic or antinociceptive drugs in traditional therapy is estimated to be about 80% of the world population. The Lamiaceae family, one of the most important herbal families, incorporates a wide variety of plants with biological and medical applications. In this study, the analgesic activity, possible active compounds of Lamiaceae genus, and also the possible mechanism of actions of these plants are presented. The data highlighted in this review paper provide valuable scientific information for the specific implications of Lamiaceae plants in pain modulation that might be used for isolation of potentially active compounds from some of these medicinal plants in future and formulation of commercial therapeutic agents.

show abstract

“…In addition, the administration of eugenol (1, 5 and 10 mg/kg) markedly decreased IL-1β levels whereas no significant effect was caused by treatment with myrcene. Similar antihypernociceptive activity was obtained with O. gratissimum essential oil (20 and 40 mg/kg) in the neurophatic pain models, providing evidence for the biological action that supports its popular use [ 79 ].…”

Section: Resultsmentioning

confidence: 56%

“…Eugenol is also the major constituent of the essential oil of Ocimum gratissimum L. (Lamiaceae), a plant popularly used in the treatment of painful diseases. In a model of neuropathic pain induced by chronic constriction of the sciatic nerve, the oral exposure to eugenol and the monoterpene myrcene (5 or 10 mg/kg, for 14 days after surgery) produced antihypernociceptive effects on male C57BL/6J mice tested in mechanical (von Frey) and thermal (hot plate) tests [ 79 ]. In addition, the administration of eugenol (1, 5 and 10 mg/kg) markedly decreased IL-1β levels whereas no significant effect was caused by treatment with myrcene.…”

Section: Resultsmentioning

confidence: 99%

Analgesic-Like Activity of Essential Oil Constituents: An Update

Sá

Lima

Nóbrega

et al. 2017

IJMS

View full text Add to dashboard Cite

The constituents of essential oils are widely found in foods and aromatic plants giving characteristic odor and flavor. However, pharmacological studies evidence its therapeutic potential for the treatment of several diseases and promising use as compounds with analgesic-like action. Considering that pain affects a significant part of the world population and the need for the development of new analgesics, this review reports on the current studies of essential oils’ chemical constituents with analgesic-like activity, including a description of their mechanisms of action and chemical aspects.

show abstract

Ocimum gratissimum Essential Oil and Its Isolated Compounds (Eugenol and Myrcene) Reduce Neuropathic Pain in Mice

Cited by 20 publications

References 40 publications

Myrcene and terpene regulation of TRPV1

Myrcene and terpene regulation of TRPV1

Medicinal Plants of the Family Lamiaceae in Pain Therapy: A Review

Analgesic-Like Activity of Essential Oil Constituents: An Update

Contact Info

Product

Resources

About