Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens

Abstract

Three Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from clinical specimens between 1992 and 2000. On the basis of 16S rRNA gene sequence similarities, these strains (CCUG 30717T, CCUG 43892 and CCUG 38531T) were shown to belong to the Alphaproteobacteria, most closely related to Ochrobactrum grignonense (99.0 and 98.2 % similarity to the type strain). Chemotaxonomic data (major ubiquinone Q-10; major polyamines spermidine, sym-homospermidine and putrescine; major polar lipids phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids C18 : 1 ω7c and C19 : 0 cyclo ω8c) supported the affiliation of the isolates to the genus Ochrobactrum. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from described Ochrobactrum species. Isolates CCUG 30717T and CCUG 43892 were closely related on the basis of DNA–DNA reassociation experiments and therefore represent one novel species, for which the name Ochrobactrum pseudogrignonense sp. nov. is proposed, with the type strain CCUG 30717T (=CIP 109451T). Isolate CCUG 38531T was different from these strains and also from other Ochrobactrum species. For this strain, the name Ochrobactrum haematophilum sp. nov. is proposed, with the type strain CCUG 38531T (=CIP 109452T).

Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant

“…S2) showed that the isolate contained the characteristic unknown aminolipid AL2, which was detected in polar lipid extracts of other members of the genus Ochrobactrum but was not produced by representatives of the closely related genus Pseudochrobactrum (Kämpfer et al , 2006, 2007b). The overall polar lipid pattern of strain MJ11 T corresponded to that described for the genus Ochrobactrum (Kämpfer et al , 2003, 2007a, 2008; Teyssier et al , 2007), and comprised large amounts of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC) and phosphatidylmonomethylethanolamine (PME) as well as moderate amounts of diphosphatidylglycerol (DPG) and unknown aminolipid AL1. Minor unknown aminolipids AL3 and AL4, phospholipids PL2 and PL4 and polar lipid L2 were also detected and have previously been found in other members of the genus Ochrobactrum (Kämpfer et al , 2007a; Huber et al , 2010).…”

“…At the time of writing, the genus Ochrobactrum comprised 16 species with validly published names, including the recently described species Ochrobactrum pecoris (Kämpfer et al , 2011). Type strains of species of the genus Ochrobactrum have been recovered from a diverse range of sources, including soil (Lebuhn et al , 2000), plants and their rhizospheres (Trujillo et al , 2005; Tripathi et al , 2006; Zurdo-Piñeiro et al , 2007; Kämpfer et al , 2008; Imran et al , 2010), industrial environments (Kämpfer et al , 2008; Huber et al , 2010), animals (Kämpfer et al , 2003, 2011) and humans (Holmes et al , 1988; Velasco et al , 1998; Teyssier et al , 2007; Kämpfer et al , 2007a). In the present paper, we describe a Gram-reaction-negative, nitrate-reducing bacterial strain, designated MJ11 T , which was isolated from sludge of a leachate treatment plant.…”

Identification and antimicrobial susceptibilities of Ochrobactrum spp.

“…O. anthropi is the clinically most important species of the genus, but sporadic cases of infections caused by O. intermedium, O. pseudintermedium, O. haematophilum, and O. pseudogrignonense have been described (Apisarnthanarak et al, 2005;Kaempfer et al, 2007b;Moeller et al, 1999;Teyssier et al, 2007;Vaidya et al, 2006). In this study, all O. anthropi, O. intermedium, O. tritici, O. grignonense, O. lupini, O. haematophilum, and O. oryzae isolates were highly resistant to all b-lactams which is consistent with the reported expression of an AmpC b-lactamase (Higgins et al, 2001).…”

Draft Genome Sequence of Ochrobactrum pseudogrignonense Strain CDB2, a Highly Efficient Arsenate-Resistant Soil Bacterium from Arsenic-Contaminated Cattle Dip Sites

“…With the full 16S sequence obtained, a new search showed a 100% match with a 1,387-bp 16S sequence from Ochrobactrum pseudogrignonense strain CCUG 30717 T (accession no. AM422371) (3). The nucleotide sequences of three other genes, groEL, gyrB , and recA (accession no.…”