Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant

Abstract

A Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11T , was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11 T belonged to the family Brucellaceae, class Alphaproteobacteria, and was most closely related to Ochrobactrum ciceri Ca-34 T (97.9 % sequence similarity) and Ochrobactrum pituitosum CCUG 50899 T (96.4 %). Comparative sequence analyses of the additional phylogenetic marker genes dnaK, groEL and gyrB confirmed the affiliation of strain MJ11 T to the genus Ochrobactrum. The G+C content of the genomic DNA of strain MJ11 T was 59.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with C 18 : 1 v7c (62.6 %) and C 19 : 0 cyclo v8c (14.2 %) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11 T to the genus Ochrobactrum. The DNA-DNA relatedness between strain MJ11 T and Ochrobactrum ciceri DSM 22292 T was 29±7 %, clearlyshowing that the isolate constitutes a new genospecies. Strain MJ11 T could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11 T represents a novel species of the genus

Ochrobactrum teleogrylli sp. nov., a pesticide-degrading bacterium isolated from the insect Teleogryllus occipitalis living in deserted cropland

“…The polyamines were extracted and then analyzed by HPLC according to the methods of Scherer and Kneifel [46]. The polyamine pattern of LCB8 T , with putrescine and spermidine as predominant compounds, 1,3-diaminopropane present as a small-amount compound and spermine as a minor component, was in agreement with polyamine compositions reported for other members of the genus Ochrobactrum [8–10].…”

“…At the time of writing, the genus Ochrobactrum comprised 17 species with validly published names, including the recently described species Ochrobactrum endophyticum [2]. Type strains of species of the genus Ochrobactrum have been recovered from a diverse range of sources, including soil [3], plants and their rhizospheres [4–7], industrial environments [6, 8, 9], animals [10, 11], and humans [1, 12–14].…”

Niches, Population Structure and Genome Reduction in Ochrobactrum intermedium: Clues to Technology-Driven Emergence of Pathogens

“…Fourty-seven percent and 41% of environmental and clinical strains of O. intermedium , respectively displayed this insertion that could be involved in the tendancy of genomic reduction observed in the population. In addition to O. intermedium and its very related phylogenetic neighbour O. ciceri [47], identical or similar insertion was also present in O. daejeonense [48], O. pituitosum [49] and very sporadically in diverse related proteobacteria rrs . Of note, atypical mobile and fast-growing Brucella strains isolated from frogs [50], [51] displayed similar insertion than O. intermedium .…”

Pesquisa de infecção por Brucella spp. em botos-vermelhos (Inia geoffrensis) de vida livre, procedentes da reserva de desenvolvimento sustentável de Mamirauá, Tefé, Amazonas, Brasil

“…O teste laboratorial considerado "padrão ouro" para o diagnóstico da brucelose é o cultivo microbiológico para isolamento de Brucella spp. O gênero Brucella tem sido considerado um gênero bacteriano em expansão, em razão das novas variantes e espécies identificadas em várias espécies de hospedeiros silvestres nas últimas décadas (BARUN et al, 2007;FOSTER et al, 2007;SCHLABRITZ-LOUTSEVITCH et al, 2009;SCHOLZ et al, 2009SCHOLZ et al, , 2010EISENBERG et al, 2013 HUBER et al, 2010), vegetais (TRUJILLO et al, 2005TRIPATHI et al, 2006;ZURDO-PINERO et al, 2007;KAMPFER et al, 2008;IMRAN et al, 2010), plantas de tratamento de esgotos e águas residuárias WOO et al, 2011), humanos (MOLLER et al, 1999;MAHMOOD et al, 2000;STIAKAKI et al, 2002;TEYSSIER et al, 2007;DHARNE et al, 2008;HAGIYA et al, 2013;MATTOS et al, 2013; SITI ROHANI E TZAR, 2013) e animais (KAMPFER et al, 2003(KAMPFER et al, , 2011EL ADAWY et al, 2012). Algumas espécies do gênero vêm sendo estudadas pelo potencial de aplicação como biorremediadores ou como bactérias promotoras de crescimentos para plantas (PANDEY et al, 2013 (MOLLER et al, 1999;MAHMOOD et al, 2000;STIAKAKI et al, 2002;DHARNE et al, 2008;HAGIYA et al, 2013;MATTOS et al, 2013;ROHANI;TZAR, 2013 (ELSAGHIR et al, 2003;HOVART et al, 2011 Atualmente, a família Propionibacteriaceae é composta por 15 gêneros bacterianos: Propionibacterium (CH...…”