Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea

Abstract

A Gram-positive, rod-shaped, spore-forming bacterium, strain S-11 T , was isolated from the activated sludge of a Bacillus-dominated wastewater treatment system in South Korea and was characterized using a polyphasic approach in order to determine its taxonomic position. Cells (0.5-0.6¾2.0-2.2 mm) were motile by means of a single subpolar flagellum. They bore ellipsoidal endospores that lay in a central position in swollen sporangia. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S-11T was a member of the genusOceanobacillus. 16S rRNA gene sequence similarity values and DNA-DNA relatedness of strain S-11 T to the type strains of other Oceanobacillus species were less than 96.2 and 66.0 %, respectively. Strain S-11 T showed distinct differences in the G+C content of the genomic DNA (33.6 mol%). The major cellular fatty acids were iso-C 14 : 0 , iso-C 15 : 0 , anteiso-C 15 : 0 and iso-C 16 : 0 . The major isoprenoid quinone was MK-7. There were also some physiological differences in comparison with the type strains of Oceanobacillus species: tests for production of acetoin and acid production from dulcitol, erythritol, myo-inositol and sorbitol were positive. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain S-11 T from the six Oceanobacillus species and subspecies with validly published names. Strain S-11 T therefore represents a novel species, for which the name Oceanobacillus caeni sp. nov. is proposed, with the type strain S-11 T (5KCTC 13061 T 5CCUG 53534 T 5CIP 109363 T ).

Jeotgalibacillus salarius sp. nov., isolated from a marine saltern, and reclassification of Marinibacillus marinus and Marinibacillus campisalis as Jeotgalibacillus marinus comb. nov. and Jeotgalibacillus campisalis comb. nov., respectively

“…The isoprenoid quinone profiles of strain ASL-1 T and members of the two genera were similar in that the predominant menaquinone was MK-7, although strain ASL-1 T , J. alimentarius and M. campisalis contained significant amounts of . The intrageneric differences in fatty acid profiles have been reported for members of some related genera (Yoon et al, 2001a;Hanada et al, 2002;Hua et al, 2007;Nam et al, 2008). Taking into account these data, it is likely to be more reasonable that the genera Jeotgalibacillus and *Summed features represent groups of two or three fatty acids that cannot be separated by GLC with the MIDI system.…”

Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor

“…oncorhynchi Yumoto et al 2005), algae (O. oncorhynchi subsp. incaldanensis Romano et al 2006), an insect (O. chironomi Raats and Halpern 2007), activated sludge (O. caeni Nam et al 2008) and food (O. kapialis Namwong et al 2009). The type strains of O. oncorhynchi subsp.…”

Oceanobacillus locisalsi sp. nov., isolated from a marine solar saltern

“…Subsequently, four Oceanobacillus species with validly published names, Oceanobacillus oncorhynchi (Yumoto et al, 2005), Oceanobacillus chironomi (Raats & Halpern, 2007), Oceanobacillus profundus (Kim et al, 2007) and Oceanobacillus caeni (Nam et al, 2008), have been reported; a further species, Oceanobacillus picturae (Lee et al, 2006), was described by the reclassification of Virgibacillus picturae (Heyrman et al, 2003). In this study, the taxonomic characterization of an Oceanobacillus-like bacterium, strain CHL-21 T , which was isolated from a marine solar saltern, is reported.…”