Jeotgalibacillus salarius sp. nov., isolated from a marine saltern, and reclassification of Marinibacillus marinus and Marinibacillus campisalis as Jeotgalibacillus marinus comb. nov. and Jeotgalibacillus campisalis comb. nov., respectively

Abstract

A Gram-variable, motile and rod-shaped bacterial strain, ASL-1T, was isolated from a marine saltern located on the coast of the Yellow Sea, Korea. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain ASL-1T clustered with Jeotgalibacillus alimentarius YKJ-13T and that this cluster joined the clade comprising the type strains of two Marinibacillus species. Strain ASL-1T exhibited 16S rRNA gene sequence similarity values of 97.3 % to J. alimentarius YKJ-13T and 96.5 % to the type strains of Marinibacillus marinus and Marinibacillus campisalis. The chemotaxonomic properties of strain ASL-1T were similar to those of one or two of the genera Jeotgalibacillus and Marinibacillus. The peptidoglycan type was A1α linked directly through l-lysine as the diamino acid. Strain ASL-1T contained MK-7 as the predominant menaquinone with the presence of a significant amount of MK-8. The predominant fatty acid was anteiso-C15 : 0. The DNA G+C content was 42.9 mol%. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain ASL-1T could be differentiated from J. alimentarius and the two Marinibacillus species. On the basis of the data presented, strain ASL-1T represents a novel species within the genus Jeotgalibacillus, for which the name Jeotgalibacillus salarius sp. nov. is proposed. The type strain is ASL-1T (=KCTC 13257T=CCUG 56751T). It is also proposed that Marinibacillus marinus and Marinibacillus campisalis be reclassified as Jeotgalibacillus marinus comb. nov. (type strain 581T=DSM 1297T=ATCC 29841T=CCUG 28884T=CIP 103308T=LMG 6930T) and Jeotgalibacillus campisalis comb. nov. (type strain SF-57T=KCCM 41644T=JCM 11810T), respectively.

Jeotgalibacillus soli sp. nov., isolated from non-saline forest soil, and emended description of the genus Jeotgalibacillus

“…All strains are positive for esterase (C4) activity and hydrolysis of aesculin and casein, but negative for indole production, methyl red and Voges-Proskauer test and hydrolysis of DNA, hypoxanthine, Tween 20 and xanthine, and negative for activity of leucine arylamidase, valine arylamidase, trypsin, a-chymotrypsin, a-galactosidase, b-glucuronidase, N-acetyl-b-glucosaminidase, a-mannosidase and a-fucosidase. All strains produce acid from D-fructose, D-glucose, D-mannitol, D-ribose, sucrose and trehalose, but not from adonitol, L-arabinose, dulcitol, myo-inositol, lactose, D-mannose, L-rhamnose, D-salicin, D-sorbitol or D-xylose a Data for the type strains of J. alimentarius and J. salarius were from Yoon et al (2001Yoon et al ( , 2010. Other data were from this study accepted criteria (Wayne et al 1987;Stackebrandt and Goebel 1994).…”

“…The genus was defined as catalase-and oxidase-positive, urease-negative, nitrate-reducing, rod-shaped bacterium, forming round endospores lying in swollen sporangia, having cell-wall peptidoglycan type A1a linked directly through L-Lys, with MK-7 and MK-8 as the predominant menaquinones and major amounts of the fatty acid iso-C15:0. A novel Jeotgalibacillus species, Jeotgalibacillus salarius (Yoon et al 2010) has been recently described, along with the transfer of Marinibacillus marinus (Rüger and Richter 1979;Rüger 1983) and Marinibacillus campisalis (Yoon et al 2004) to the genus as Jeotgalibacillus marinus and Jeotgalibacillus campisalis, respectively. The four recognized Jeotgalibacillus species were isolated either from the seefood jeotgal (J. alimentarius) or marine saltern (J. campisalis, J. salarius) or sea sediments (J. marinus).…”

Draft genome of Jeotgalibacillus campisalis SF-57T, a moderate halophilic bacterium isolated from marine saltern

“…The cell wall peptidoglycan contains L-lysine at position three of the peptide subunit and predominant menaquinones of MK-7 and MK-8. The genus Jeotgalibacillus is represented by six species; Jeotgalibacillus marinus (Yoon et al, 2010), Jeotgalibacillus campisalis (Yoon et al, 2010), Jeotgalibacillus soli (Cunha et al, 2012), Jeotgalibacillus alimentarius (Yoon et al, 2001), and Jeotgalibacillus malaysiensis (Yaakop et al, in press). Jeotgalibacillus spp.…”

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

“…In this study, the use of 16S rDNA sequences as a main tool to identify halophilic bacteria demonstrate that this method is helpful since 96 isolates (77.5%) were identified at the species level with ≥ 99% similarity with their relative reference strains. Moreover, it turned out that four of the strains recovered in this study belonged to Bacillus oceanisediminis , Jeotgalibacillus salarius (Yoon et al, 2010), Vibrio atypicus and Psychrobacter piscatorii (Yumoto et al, 2010) which were described in 2010 as new species, and B. oceanisediminis is one of the dominating species (Table 2).…”