Occurrence, Pathogenicity, and Mycotoxin Production of Fusarium temperatum in Relation to Other Fusarium Species on Maize in Germany

Pfordt, Annette; Schiwek, Simon; Rathgeb, Anna; Rodemann, Charlotte; Bollmann, Nele; Buchholz, Matthias; Karlovský, Petr; Tiedemann, Andreas von

doi:10.3390/pathogens9110864

Cited by 21 publications

(17 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…F. graminearum was detected in ≥ 80% of all Fusarium head blight (FHB) samples, sometimes even 100% 38 . In addition, the frequency of F. graminearum isolated from ears ranged from 30 to 71% with an average of 57%, and from stalks ranged from 43 to 81%, with the average of 65% 39 . Although the isolation frequency of F. graminearum from CFSR was only 3.65% in our present study, the potential risk of mycotoxins produced by F. graminearum to human health cannot be ignored 40 .…”

Section: Discussionmentioning

confidence: 99%

Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

Wang

Sun

et al. 2021

Sci Rep

View full text Add to dashboard Cite

To elucidate the symptoms and pathogens diversity of corn Fusarium sheath rot (CFSR), diseased samples were collected from 21 county-level regions in 12 prefecture-level districts of Sichuan Province from 2015 to 2018 in the present study. In the field, two symptom types appeared including small black spots with a linear distribution and wet blotches with a tawny or brown color. One hundred thirty-seven Fusarium isolates were identified based on morphological characteristics and phylogenetic analysis (EF1-α), and Koch’s postulates were also assessed. The results identified the isolates as 8 species in the Fusarium genus, including F. verticillioides, F. proliferatum, F. fujikuroi, F. asiaticum, F. equiseti, F. meridionale, F. graminearum and F. oxysporum, with isolation frequencies of 30.00, 22.67, 15.33, 7.33, 6.00, 5.33, 3.33 and 1.33%, respectively. Fusariumverticillioides and F. proliferatum were the dominant and subdominant species, respectively. Two or more Fusarium species such as F. verticillioides and F. proliferatum were simultaneously identified at a mixed infection rate of 14.67% in the present study. The pathogenicity test results showed that F. proliferatum and F. fujikuroi exhibited the highest virulence, with average disease indices of 30.28 ± 2.87 and 28.06 ± 1.96, followed by F. equiseti and F. verticillioides, with disease indices of 21.48 ± 2.14 and 16.21 ± 1.84, respectively. Fusarium asiaticum, F. graminearum and F. meridonale showed lower virulence, with disease indices of 13.80 ± 2.07, 11.57 ± 2.40 and 13.89 ± 2.49, respectively. Finally, F. orysporum presented the lowest virulence in CFSR, with a disease index of 10.14 ± 1.20. To the best of our knowledge, this is the first report of F. fujikuroi, F. meridionale and F. asiaticum as CFSR pathogens in China.

show abstract

Section: Discussionmentioning

confidence: 99%

Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

Wang

Sun

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…graminearum and F . verticillioides when isolated from naturally infected maize ears, with an even higher aggressiveness than the other two species (Pfordt, Schiwek, et al, 2020). Again, this example shows that we always have to be aware of Fusarium species shifts and that different Fusarium species colonize maize kernels at the same time (Reyes Gaige et al, 2020), including respective associated changes of mycotoxin profiles in maize kernels.…”

Section: Maize Ear Rots and Related Mycotoxin Productionmentioning

confidence: 99%

Global warming and increasing maize cultivation demand comprehensive efforts in disease and insect resistance breeding in north‐western Europe

Miedaner

Juroszek²

2021

Plant Pathology

View full text Add to dashboard Cite

Maize productivity is threatened by global climate change. Climate change scenarios suggest that north‐western (NW) Europe will get warmer and drier during the main crop‐growing period. In general, more northerly regions will benefit, whereas more southerly regions will suffer suboptimal rain‐fed farming conditions. In these latter regions in particular, the resulting probable lower realized on‐farm maize grain and biomass yields must be safeguarded. Breeding for resistance against already existing and emerging diseases and insect pests is one component to achieve yield stability across years. Durable multiple‐disease resistance will become especially crucial. Herein, we focus on disease resistance breeding approaches in maize, especially related to northern corn leaf blight and Fusarium ear rots, although virus and bacterial diseases will become more important as well. Continuous adjustments of disease resistance breeding strategies will be required. Insect pest resistance breeding must be improved considerably, as in a warmer world insects will thrive, probably causing detrimental direct (feeding, sucking, etc.) and indirect (vectors of pathogens, feeding wounds creating gateways for many pathogens, passive transport of inoculum across maize plants) effects. Four case studies on insects that are already prevalent in NW Europe or may be expected in the near future are covered in this review. Maize cultivars need to combine both durable multiple‐disease and multiple‐insect resistance, although the implementation of many different effective resistance resources in breeding programmes will be challenging, particularly if trade‐offs among breeding goals appear.

show abstract

“…IMATHIU et al (2009) confirmed successful infection and disease development by all examined F. langsethiae isolates. According to PFORDT et al (2020) all observed isolates of F. temperatum and F. subglutinans affected winter wheat and caused moderate to severe FHB symptoms. OPOKU et al (2011) were assessing the aggressiveness of 20 different F. langsethiae isolates on wheat leaves by measuring the lesion length formerd at the inoculation site.…”

Section: Resultsmentioning

confidence: 99%

The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain

Savic

Nikolić

et al. 2021

Genetika

View full text Add to dashboard Cite

During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.

show abstract

Occurrence, Pathogenicity, and Mycotoxin Production of Fusarium temperatum in Relation to Other Fusarium Species on Maize in Germany

Cited by 21 publications

References 75 publications

Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

Global warming and increasing maize cultivation demand comprehensive efforts in disease and insect resistance breeding in north‐western Europe

The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain

Contact Info

Product

Resources

About