Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

Wang, Wei; Wang, Bo; Sun, Xin; Qi, Xiaobo; Zhao, Conghao; Chang, Xiaoli; Khaskheli, Muhammad Ibrahim; Gong, Guoshu

doi:10.1038/s41598-021-82463-2

Cited by 15 publications

(9 citation statements)

References 44 publications

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“…Corn and pepper are important and representative food and vegetable crops. The most economically important diseases affecting these crops are blights disease, anthracnose disease, and damping-off disease [ 34 ]. Particularly, pathogenic Fusarium fungal species infect corn plants and reduce yields worldwide.…”

Section: Discussionmentioning

confidence: 99%

Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi

Kim

Song²,

Kim³

et al. 2022

JoF

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The use of synthetic fungicides has caused major problems such as soil and water pollution and negatively affects non-target species. Microbial biocontrol agents are needed for crop disease management to reduce agrochemical use. Bacillus and related genera produce secondary metabolites with agricultural applications, such as the pathogen-control agent Bacillus velezensis. We isolated B. velezensis TSA32-1 from soil and identified its characteristics by sequencing its 16S rRNA. B. velezensis TSA32-1 showed enzyme activity and antimicrobial effects against phytopathogenic fungi by inhibiting the growth of Fusarium graminearum, F. fujikuroi, Alternatia alternate, and Diaporthe actinidiae. Additionally, B. velezensis TSA32-1 protected diseases in corn and pepper seeds caused by F. graminearum and Pythium ultimum. The complete genome of B. velezensis TSA32-1 was 4.05 Mb with a G+C content of 46.3 mol % and possessed the bacillaene biosynthesis cluster, a polyketide that inhibits protein biosynthesis. We also detected a surfactin synthesis cluster, known as non-ribosomal peptide synthetases, which biosynthesizes the antibacterial substance lipopeptide. Surfactin, and fengycin family compounds, secondary metabolites known as key factors in biological control, also detected B. velezensis TSA32-1 which shows potential as a biocontrol agent for controlling plant pathogens in agriculture.

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Section: Discussionmentioning

confidence: 99%

Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi

Kim

Song²,

Kim³

et al. 2022

JoF

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“…The variability within F. proliferatum isolates might be due to the species causing various types of diseases and differences in the hosts infected or substrates occupied (Leslie, 1995) as well as their ability to adapt and evolve in various environmental conditions (Medina et al, 2017). Fusarium proliferatum has also been reported to cause diseases on various crops including cereal grains (Mielniczuk & Skwarylo-Bednarz, 2020), corn (Scarpino et al, 2015;Wang et al, 2021), asparagus (Stepien et al, 2016Djalali Farahani-Kofoet et al, 2020), dragon-fruits (Masratul Hawa et al, 2013), pineapple (Nurul Faziha et al, 2016), garlic (Mondali et al, 2021, mango (Omar et al, 2018) and even infected humans (Herbrecht et al, 2004;Sun et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

MOLECULAR CHARACTERIZATION AND MATING STUDY OF Fusarium proliferatum FROM VARIOUS HOSTS IN MALAYSIA

Azuddin

Mohd

Nor

et al. 2021

MABJ

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Fusarium proliferatum is a well-known plant pathogenic fungus-infected many crops. The present study was carried out to molecularly identified and characterized morphologically identified F. proliferatum from various hosts and substrates. The species identity of the isolates was verified as F. proliferatum based on TEF-1α sequences and phylogenetic analysis indicated high intraspecific variations. RFLP-IGS analysis also indicated high intraspecific variations of which the isolates were clustered into three RFLP Groups (I, II, and III) comprising 67 IGS haplotypes. Seventy isolates were crossed-fertile and proven to be members of mating population D (MP-D) of Gibberella fujikuroi while four isolates were infertile. A high level of intraspecific variations is vital for F. proliferatum adaptation and survival in the host and environment. Correct species identification of F. proliferatum is important as the fungus is a well-known plant pathogen and mycotoxin producer. Correct species identity is also essential to strategize suitable disease control methods as well as to predict their host range and mycotoxin production.

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“…Fusarium pathogens often appear in high-humidity and high-heat areas and Fusarium head blight (FHB) has reached historically high epidemic acreages in the middle and lower reaches of the Yangtze River ( Wang et al, 2011 ; Yang et al, 2021 ). It is also one of the major diseases in wheat and maize planting areas in the Northeast Plain, North China Plain, and Sichuan Basin ( Feng et al, 2011 ; Wei et al, 2013 ; Wang et al, 2021 ). Thus, Fusarium poses a great threat to China’s food production ( Gong et al, 2009 ; Qiu and Shi, 2014 ; Qiu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

An Efficient Strategy Combining Immunoassays and Molecular Identification for the Investigation of Fusarium Infections in Ear Rot of Maize in Guizhou Province, China

Shang

et al. 2022

Front. Microbiol.

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Fusarium is one of the most important phytopathogenic and mycotoxigenic fungi that caused huge losses worldwide due to the decline of crop yield and quality. To systematically investigate the infections of Fusarium species in ear rot of maize in the Guizhou Province of China and analyze its population structure, 175 samples of rotted maize ears from 76 counties were tested by combining immunoassays and molecular identification. Immunoassay based on single-chain variable fragment (scFv) and alkaline phosphatase (AP) fusion protein was first employed to analyze these samples. Fusarium pathogens were isolated and purified from Fusarium-infected samples. Molecular identification was performed using the partial internal transcribed spacer (ITS) and translation elongation factor 1α (TEF-1α) sequences. Specific primers were used to detect toxigenic chemotypes, and verification was performed by liquid chromatography tandem mass spectrometry (LC–MS/MS). One-hundred and sixty three samples were characterized to be positive, and the infection rate was 93.14%. Sixteen species of Fusarium belonging to six species complexes were detected and Fusarium meridionale belonging to the Fusarium graminearum species complex (FGSC) was the dominant species. Polymerase chain reaction (PCR) identification illustrated that 69 isolates (56.10%) were potential mycotoxin-producing Fusarium pathogens. The key synthetic genes of NIV, NIV + ZEN, DON + ZEN, and FBs were detected in 3, 35, 7, and 24 isolates, respectively. A total of 86.11% of F. meridionale isolates carried both NIV- and ZEN-specific segments, while Fusarium verticillioides isolates mainly represented FBs chemotype. All the isolates carrying DON-producing fragments were FGSC. These results showed that there are different degrees of Fusarium infections in Guizhou Province and their species and toxigenic genotypes display regional distribution patterns. Therefore, scFv-AP fusion-based immunoassays could be conducted to efficiently investigate Fusarium infections and more attention and measures should be taken for mycotoxin contamination in this region.

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Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

Cited by 15 publications

References 44 publications

Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi

Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi

MOLECULAR CHARACTERIZATION AND MATING STUDY OF Fusarium proliferatum FROM VARIOUS HOSTS IN MALAYSIA

An Efficient Strategy Combining Immunoassays and Molecular Identification for the Investigation of Fusarium Infections in Ear Rot of Maize in Guizhou Province, China

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