MOLECULAR CHARACTERIZATION AND MATING STUDY OF Fusarium proliferatum FROM VARIOUS HOSTS IN MALAYSIA

Abstract

Fusarium proliferatum is a well-known plant pathogenic fungus-infected many crops. The present study was carried out to molecularly identified and characterized morphologically identified F. proliferatum from various hosts and substrates. The species identity of the isolates was verified as F. proliferatum based on TEF-1α sequences and phylogenetic analysis indicated high intraspecific variations. RFLP-IGS analysis also indicated high intraspecific variations of which the isolates were clustered into three RFLP Groups (I, II, and III) comprising 67 IGS haplotypes. Seventy isolates were crossed-fertile and proven to be members of mating population D (MP-D) of Gibberella fujikuroi while four isolates were infertile. A high level of intraspecific variations is vital for F. proliferatum adaptation and survival in the host and environment. Correct species identification of F. proliferatum is important as the fungus is a well-known plant pathogen and mycotoxin producer. Correct species identity is also essential to strategize suitable disease control methods as well as to predict their host range and mycotoxin production.

The Host Range of Fusarium proliferatum in Western Canada

“…Molecular identification and phylogenetic analysis based on the translation elongation factor 1-alpha (TEF-1α) sequences, along with mating studies on multiple isolates from diverse hosts and locations, have suggested that there is no relation between F. proliferatum isolates and their hosts or geographic origins [18,37,55,56]. Nevertheless, an evaluation of vegetative compatibility among isolates of F. proliferatum indicated that isolates recovered from maize, onion, sugarcane, and rice could be classified into different vegetative compatibility groups (VCGs), indicating a correlation between VCGs and host preferences [12].…”