Occurrence of β-lactam-resistant Staphylococcus aureus in milk from primiparous dairy cows in the northeastern region of Brazil

Silva, Amanda T F; Silva, José G da; Aragão, Breno Bezerra; Peixoto, Rodolfo de Moraes; Mota, Rinaldo Aparecido

doi:10.1007/s11250-020-02259-w

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…The data for the USA and Germany are similar, as they are at the level of 41.2% and 47.1%, respectively [7]. On the other hand, information showing this frequency in Brazil is 74.07% [8]. Staphylococcus aureus borderline resistant to oxacillin, an antibiotic of the beta-lactam group, is described.…”

Section: Discussion Of the Problemmentioning

confidence: 77%

“…Another example is the study from Pakistan where the gen mecA occurred with a frequency of 30% [11]. For comparison, in Germany and Italy its incidence is much lower (6.9% and 5.9%, respectively) [8]. A higher rate for European countries is reported in a study on the prevalence of the mecA gene in Greece, which was 10% there [12].…”

Section: Discussion Of the Problemmentioning

confidence: 99%

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Properties of coagulase-positive staphylococcal cells that make it difficult to diagnose and treat mastitis in cows

Grzeczka

Niewitecki

2021

Medical Journal of Cell Biology

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Coagulase-positive staphylococci are a group of bacteria that, among other things, cause inflammation of the udder in cows (mastitis). The identification of the species of staphylococcus causing the inflammation is crucial for the success of the treatment process due to the individual characteristics of the strains and even the characteristics of the bacterial population in the herd. However, through evolution, staphylococci have developed a number of processes that make species identification difficult. Knowledge of the actual cause of inflammation enables the selection of appropriate therapy, but despite advanced diagnostic techniques, erroneous results still occur. In this review, we present the current knowledge of mainly resistance mechanisms and how they affect the drug resistance of microorganisms. We also highlight the difficulties in the diagnosis and treatment of bovine mastitis.

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Section: Discussion Of the Problemmentioning

confidence: 77%

Section: Discussion Of the Problemmentioning

confidence: 99%

Properties of coagulase-positive staphylococcal cells that make it difficult to diagnose and treat mastitis in cows

Grzeczka

Niewitecki

2021

Medical Journal of Cell Biology

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“…Another issue that makes the bacteria in milk important for healthcare is the potential to carry antimicrobial resistance genes, because antibiotic consumption in animal husbandry can be unconscious in most developing countries [ 47 , 48 ]. Antibiotic resistance is not only a major crisis for non-healthy animals (with mastitis), but also a potential problem for healthy animals.…”

Section: Discussionmentioning

confidence: 99%

The Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milk

et al. 2023

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Staphylococci is an opportunistic bacterial population that is permanent in the normal flora of milk and poses a serious threat to animal and human health with some virulence factors and antibiotic-resistance genes. This study was aimed at identifying staphylococcal species isolated from raw milk and to determine hemolysis, biofilm, coagulase activities, and beta-lactam resistance. The raw milk samples were collected from the Düzce (Türkiye) region, and the study data represent a first for this region. The characterization of the bacteria was performed with MALDI-TOF MS and 16S rRNA sequence analysis. The presence of coa, icaB, blaZ, and mecA was investigated with PCR. A nitrocefin chromogenic assay was used for beta-lactamase screening. In this context, 84 staphylococci were isolated from 10 different species, and the dominant species was determined as S. aureus (32.14%). Although 32.14% of all staphylococci were positive for beta hemolysis, the icaB gene was found in 57.14%, coa in 46.42%, mecA in 15.47%, and blaZ in 8.33%. As a result, Staphylococcus spp. strains that were isolated from raw milk in this study contained some virulence factors at a high level, but also contained a relatively low level of beta-lactam resistance genes. However, considering the animal–environment–human interaction, it is considered that the current situation must be monitored constantly in terms of resistance concerns. It must not be forgotten that the development of resistance is in constant change among bacteria.

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“…Silva et al [ 33 ] reported oxacillin-resistant S. aureus isolates from the milk of primiparous dairy cows in the northeastern region of Brazil, and most of the isolates carried the blaZ gene, suggesting its importance as an inducer of beta-lactam resistance. Nevertheless, none of our isolates from primiparous dairy cows carried the blaZ gene or efflux pump genes.…”

Section: Discussionmentioning

confidence: 99%

Genetic and Phenotypic Characterization of Subclinical Mastitis-Causing Multidrug-Resistant Staphylococcus aureus

Silva,

Gonçalves,

Dantas

et al. 2023

Antibiotics

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The core objective of this study was to genetically and phenotypically characterize subclinical mastitis-causing multidrug-resistant Staphylococcus aureus (MDRSA). In addition, risk factors associated with subclinical mastitis caused by MDRSA were investigated. Bacterial cultures were performed on 2120 mammary quarters, 40 swabs of milk utensils, 5 bulk tank milk samples, and 11 nostril and 11 hand swabs from milkers from five dairy farms. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was conducted for S. aureus identification. Antimicrobial resistance was screened phenotypically using the disk diffusion test in all S. aureus isolates. A biofilm formation assay; detection of genes associated with beta-lactam resistance, efflux pump, and biofilm formation; and pulsed-field gel electrophoresis (PFGE) were performed in all MDRSA isolates. Multi-locus sequence typing (MLST) was carried out in cefoxitin-resistant MDRSA isolates. A total of 188 S. aureus isolates from milk as well as two from milking utensils and one from bulk tank milk were identified. Most of the isolates (92.7%; 177 of 191) showed beta-lactam resistance, and 7% (14 of 191) were MDRSA. Interestingly, 36% (5 of 14) of MDRSA isolates were cefoxitin-resistant, but none carried mecA or mecC genes. Based on PFGE results, it was observed that S. aureus strains were more likely to be unique to a specific herd. Two clonal complexes were identified, CC97 (ST126; commonly livestock-associated) and CC1 (ST7440; usually community-associated). To the best of our knowledge, this is the first report of ST7440 isolated from bovine mastitis in Brazil. The risk factor results underscored the importance of considering parity, stage of lactation, SCC, milk production, and herd size when studying the risk of subclinical mastitis and antimicrobial resistance in S. aureus. Thus, to implement effective strategies to prevent subclinical mastitis in dairy herds and to minimize MDRSA spread, it is important to understand MDRSA strains’ distribution and their antimicrobial resistance profile.

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Occurrence of β-lactam-resistant Staphylococcus aureus in milk from primiparous dairy cows in the northeastern region of Brazil

Cited by 9 publications

References 25 publications

Properties of coagulase-positive staphylococcal cells that make it difficult to diagnose and treat mastitis in cows

Properties of coagulase-positive staphylococcal cells that make it difficult to diagnose and treat mastitis in cows

The Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milk

Genetic and Phenotypic Characterization of Subclinical Mastitis-Causing Multidrug-Resistant Staphylococcus aureus

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