Occurrence of Staphylococcus aureus in cattle, sheep, goat, and pig rearing in the Czech Republic

Klimešová, Marcela; Manga, Ivan; Nejeschlebová, Ludmila; Horáček, J.; Ponížil, Antonín; Vondrušková, Eva

doi:10.2754/avb201786010003

Cited by 16 publications

(15 citation statements)

References 28 publications

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“…We found high levels of resistance to gentamycin and ciprofloxacin in milk and human isolates (Table -9), which agreed with the findings of Hoque et al, Zeinhom et al,Ameen et al,Reta et al and Klimešová et al [30,35,61,62]. These authors also detected high resistance to gentamycin in animal [63] in Egypt found increased resistance in S. aureus isolates from clinical mastitis to penicillin and gentamicin.…”

Section: Resultssupporting

confidence: 91%

Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt

et al. 2021

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Background and Aim: Milk is a chief source of many nutrients. However, we must also bear in mind that it is a potential source for many cases of food poisoning. This study was conducted to investigate the prevalence of cow mastitis and evaluate the presence of enterotoxins and antibiotic resistance patterns in Staphylococcus aureus isolated from milk and contact humans in El-Behira Province, Egypt. Materials and Methods: A total of 680 milk samples from 170 cows and 86 human samples consisting of 43 hand swabs and 43 nasal swabs were analyzed. The milk samples were subjected to the California mastitis test. Results: The general occurrence was 23.1% (157/680) where 48 quarters had clinical mastitis and 109 had subclinical mastitis. Subsequently, S. aureus was isolated in Baird-Parker agar where typical and atypical colonies were selected and submitted to coagulase and complementary tests. Out of 48 samples of mastitic milk studied, 16 (33.3%) showed contamination by S. aureus whereas 109 samples of subclinical mastitis showed contamination in only 18 (16.5%). On the opposite hand, of the 86 human samples, 33 revealed S. aureus contamination, corresponding to 38.37% of the samples. Furthermore, multiplex polymerase chain reaction targeting nuc and the staphylococcal enterotoxin-encoding genes sea, seb, sec, sed, and see were performed after culture, revealing that 88.2% (30/34) of milk samples and 93.9% (31/33) of human samples were variably positive to those genes. Conclusion: The use of nuc gene based PCR is an accurate and rapid method for S aureus isolates detection. A high prevalence of multiple drug-resistant isolates of S. aureus recovered from both human and milk represents further evidence for possible veterinary hazards as well as public hazards, especially to those that consume milk from this region.

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Section: Resultssupporting

confidence: 91%

Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt

et al. 2021

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“…The resistance (40.63%) of the isolates to tetracycline in this study may reflect the excessive use of the drug in treatment in the study area. The susceptibility by S. aureus isolates to chloramphenicol, gentamicin and kanamycin observed in this study is comparable with the findings of Klimesova et al (2017) who reported resistance to chloramphenicol to be 15.63 %. The high proportion of susceptibility by the isolates to nitrofurantoin (84.38%), ciprofloxacin (78.13%) and chloramphenicol (78.13%) is suggestive of the potential efficacy of these drugs in treating MRSA infections and may reflect the fact that they may be less commonly abused in the environment.…”

Section: Resultssupporting

confidence: 90%

Occurrence of mastitis and methicillin resistant <i>Staphylococcus aureus</i> in goats in Zaria, Kaduna State, Nigeria

Udoh¹,

Kwaga²,

Umoh³

et al. 2019

Nig. Vet. J.

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“…This behavior can be performed on self-grooming or on another animal (allogrooming) and, due to this behavior, it is possible that the animals in this work have crossed contamination, justifying the high resistance index of these samples. [38][39][40] The origin of to antimicrobials is not completely defined and may have different genetic origins, for example, the genes encoding blaZ resistance 31,32 which, like mecA, 33 are responsible for resistance to penicillins. Therefore, in this work, the presence of the mecA gene in oxacillin-resistant isolates was investigated and no positive sample was detected.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance profile of gram‐positive isolates belonging to the microbiota of non‐human primates

Zaniolo

Santos

Bondezan

et al. 2022

J of Medical Primatology

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Background The aim of this work was to identify gram‐positive bacteria and their respective resistance profiles of free‐living capuchin monkeys. Methods For this, 15 Sapajus nigritus were captured in a municipal urban park in the northern region of the state of Paraná, Brazil, and under pharmacological restraint, samples were collected with sterile swabs from the oral, rectal, ocular, nasal, and auricular regions. After isolation of the 22 gram‐positive bacteria, each isolate was subjected to the catalase and coagulase tests for presumptive identification. Subsequently, phenotypic tests for bacterial resistance were performed using the agar diffusion disc method. The samples resistant to oxacillin were submitted to the PCR technique to search for the mecA gene. Results Of the 22 gram‐positive cocci of these two (9.09%) are Streptococcus spp. and twenty (90.91%) Staphylococcus spp. Among Staphylococcus spp. three (13.64%) were coagulate‐negative (CoNS) and seventeen (86.36%) coagulate‐positive (CoPS). Of the antimicrobials tested, enrofloxacin had the best performance, with only one (04.54%) isolate resistant to it, on the other hand, the antimicrobials with the lowest performance were cefotaxime and penicillin with 19 (82.36%) and 18 (81.81%) resistant isolates, respectively. Only five isolates had MAR less than 0.2, being one ocular, one oral, and three nasal, they had multiple resistance index varied between 0.07 and 0.92, with an average of 0.45 and a mode of 0.3. Among the samples with the highest resistance index, a positive coagulase Staphylococcus stood out, being intermediate to gentamicin and resistant to other antibiotics and an intermediate streptococcus to gentamicin, enrofloxacin, and resistant to other antibiotics. No sample was positive to mecA gene. Conclusions Future studies should be conducted to identify the Staphylococcus species, the high rate of antimicrobial resistance of the monkeys in this study suggests that Grooming's behavior may be contributing to the sharing of the resistant microorganism among the members of this group of primates.

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Occurrence of Staphylococcus aureus in cattle, sheep, goat, and pig rearing in the Czech Republic

Cited by 16 publications

References 28 publications

Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt

Molecular detection of Staphylococcus aureus enterotoxin genes isolated from mastitic milk and humans in El-Behira, Egypt

Occurrence of mastitis and methicillin resistant <i>Staphylococcus aureus</i> in goats in Zaria, Kaduna State, Nigeria

Antimicrobial resistance profile of gram‐positive isolates belonging to the microbiota of non‐human primates

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