Occurrence of Salmonella infection and antimicrobial susceptibility for local Salmonella isolates from different sources in a cross-sectional study

Abstract

Salmonellosis is a considerable public health problem worldwide, with high economic importance in developed countries. The main purpose of this study was to determine the prevalence of Salmonella infection and antibiogram analysis of isolated strains in a cross-sectional study in Egypt 2016-2017. The study investigated twenty-eight Salmonella isolates from different areas in Egypt and different types of samples, such as human stool (9.3%), Egyptian cattle egrets and storks (28.5%) and grilled chicken from electric grills (36.6%). No isolates were detected from grilled chicken from charcoal grills or drinking water. The main Salmonella serotype detected in the isolates was S. typhimurium (86.5%). Molecular characterization of the invA gene by PCR was carried out and then confirmed by sequencing, and the results were submitted to GenBank. Antibiogram analysis of Egyptian isolates carried out on 9 antimicrobial discs reported that the routine regimes of treatment were not yet effective for recent new Salmonella generations in 2016-2017. The new isolates could be treated with levofloxacin, cefaperazone/sulbactam, chloramphenicol, imipenem or meropenem.

Prevalence and molecular characterization of foodborne and human‐derived Salmonella strains for resistance to critically important antibiotics

“…Salmonella was detected in 4.83% (7/145) of human samples, which falls between the relatively low (4%) and high (9.3 and 12.6%) percentages reported in previous studies conducted in the Egyptian provinces of Beni‐Suef (Hassan et al., 2016), Menoufia and Cairo (Abdelmalek et al., 2019), as well as Al‐Qalyubia (Abd El Samie et al., 2018). The most well‐known Typhoid disaster in Al‐Qalyubia occurred in El Qanater El Khayreya City in 2009, and was caused by sewage mixing with drinking water, resulting in up to 164 Typhoid cases.…”

“…There is a lack of publications in the literature devoted to this type of research for Egypt. Nevertheless, in other provinces, two cross‐sectional studies recorded higher total Salmonella occurrence rates of 11.1% (78/700) (Ahmed et al., 2016) and 16% (Abdelmalek et al., 2019).…”

“…Past Egyptian cross‐sectional studies compared the presence of Salmonella in broiler meat and/or farms, and human samples (Abdelmalek et al., 2019; El‐Sharkawy et al., 2017; Hassan et al., 2016). However, little is known or published about the prevalence of this pathogen in different food types (Ahmed & Shimamoto, 2014), particularly raw cattle products and ready‐to‐eat/drink food (RTE/D), compared to human cases in the Al‐Qalyubia governorate, including assessment of isolated Salmonella species for susceptibility to new critical important antibiotics.…”

Sequencing and phylogenetic analysis of the stn gene of Salmonella species isolated from different environmental sources at Lake Qarun protectorate: The role of migratory birds and public health importance

“…In Egypt, foodborne pathogens, such as Salmonella , pose substantial health risks [ 31 ]. According to the World Health Organization [ 32 ], Salmonella species account for the third-highest number of deaths among the 31 etiological agents of diarrhea and/or infectious diseases.…”

Use of Quorum Sensing Gene SdiA as a Molecular Marker for Salmonella Diagnosis

“…Twenty-eight local Egyptian Salmonella isolates from the Cairo, Giza and Menoufia governorates were collected from different sources, such as 75 human stool from clinically affected persons tested by the Widal test, 35 Egyptian stork (major Salmonella carriers) and 30 from multilayer electric grills, grilled chicken. All strains of Salmonella were characterized culturally, biochemically, serologically and molecularly by using a PCR assay for the invA gene as previously studied by (Abdelmalek et al, 2019), as it is the most common PCR assay for Salmonella isolate identification,. Four Local non-Salmonella bacterial isolates were used as negative controls for the sdiA gene as follows: E. coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa.…”